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| Registros recuperados : 111 | |
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Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
03/02/2012 |
|
Data da última atualização: |
06/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SANTANA, J. R. F. de; PAIVA, R.; SOUZA, A. V. de; OLIVEIRA, L. M. de. |
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Afiliação: |
JOSÉ RANIERE FERREIRA DE SANTANA; RENATO PAIVA; ANA VALERIA DE SOUZA, CPATSA; LENALDO MUNIZ DE OLIVEIRA. |
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Título: |
Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 35, n. 5, p. 916-923, set./out. 2011. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as “araticum”. Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoa |
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Palavras-Chave: |
Abscisão foliar; Cultivo in vitro; Tampão de algodão. |
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Thesagro: |
Annona glabra; Araticum; Fruta Tropical. |
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Thesaurus NAL: |
Fruits; Leaf abscission. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53397/1/Ana-Valeria-2011.pdf
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Marc: |
LEADER 02308naa a2200253 a 4500
001 1914333
005 2023-06-06
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTANA, J. R. F. de
245 $aEffect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.$h[electronic resource]
260 $c2011
520 $aThe present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as “araticum”. Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoa
650 $aFruits
650 $aLeaf abscission
650 $aAnnona glabra
650 $aAraticum
650 $aFruta Tropical
653 $aAbscisão foliar
653 $aCultivo in vitro
653 $aTampão de algodão
700 1 $aPAIVA, R.
700 1 $aSOUZA, A. V. de
700 1 $aOLIVEIRA, L. M. de
773 $tCiência e Agrotecnologia$gv. 35, n. 5, p. 916-923, set./out. 2011.
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