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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
25/03/2002 |
Data da última atualização: |
25/03/2002 |
Autoria: |
KELEMU, S.; BADEL, J. L.; MORENO, C. X.; MILES, J. W.; CHAKRABORTY, S.; FERNANDES, C. D.; CHARCHAR, M. J. D' A. |
Título: |
Biodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. I. genetic and pathogenic diversity in Colletotrichum gloeosporioides isolates from Stylosanthes guianensis. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Tropical Grasslands, Queensland, v. 31, p. 387-392, 1997. |
Idioma: |
Inglês |
Conteúdo: |
Anthacnose, caused by Colletotrichum gloeosporioides, is the most important and widespread disease of Stylosanthes, a diverse tropical and subtropical forage legume naturally disributed in central and South America. This paper compares the genetic and pathogenic diversity of 45 isolates, which originated from S. guianensis genotypes. Published information on the pathogenic diversity of these isolates on a set of 12 S. guianensis was used. The amount of genetic diversity was measured at molecular level by polymerase chain reaction amplifications of DNA with 9 arbitrary primers of 10 bases each using the Random Amplified Polymorphic DNA (RAPD) ,markers. The amplifications revealed scorable polymorphism among the isolates, and a total of 80 band positions were scored. Using Ward s method (N igual 6) of statistical analysis, the isolates were separated into 6 clusters. Generally, isolates were clustered togheder by their geographic origin and/or their original host genotype. Isolates from Carimagua, Colombia, a savanna ecosystem and a long-time Stylosanthes breeding and selection site, exhibited a relatively wider range of genetic diversity than those from a newly opened trial site in the Amazon basin of Colombia. No strict correlation existed between genetic diversity, as measured by RAPD, and differential virulence, as defined by the pathotype. Isolates of the same pathotype/RAPD grouping often originated from the same host genotype and/or geographical locality. This information will be useful in determining future sampling strategies for the pathogen population. MenosAnthacnose, caused by Colletotrichum gloeosporioides, is the most important and widespread disease of Stylosanthes, a diverse tropical and subtropical forage legume naturally disributed in central and South America. This paper compares the genetic and pathogenic diversity of 45 isolates, which originated from S. guianensis genotypes. Published information on the pathogenic diversity of these isolates on a set of 12 S. guianensis was used. The amount of genetic diversity was measured at molecular level by polymerase chain reaction amplifications of DNA with 9 arbitrary primers of 10 bases each using the Random Amplified Polymorphic DNA (RAPD) ,markers. The amplifications revealed scorable polymorphism among the isolates, and a total of 80 band positions were scored. Using Ward s method (N igual 6) of statistical analysis, the isolates were separated into 6 clusters. Generally, isolates were clustered togheder by their geographic origin and/or their original host genotype. Isolates from Carimagua, Colombia, a savanna ecosystem and a long-time Stylosanthes breeding and selection site, exhibited a relatively wider range of genetic diversity than those from a newly opened trial site in the Amazon basin of Colombia. No strict correlation existed between genetic diversity, as measured by RAPD, and differential virulence, as defined by the pathotype. Isolates of the same pathotype/RAPD grouping often originated from the same host genotype and/or geographical locality. This informati... Mostrar Tudo |
Palavras-Chave: |
Biodversity. |
Thesagro: |
Biodiversidade; Colletotrichum Gloeosporioides; Patogenicidade; Stylosanthes Guianensis. |
Thesaurus Nal: |
pathogenicity. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02447naa a2200265 a 4500 001 1564554 005 2002-03-25 008 1997 bl --- 0-- u #d 100 1 $aKELEMU, S. 245 $aBiodiversity, epidemiology and virulence of Colletotrichum gloeosporioides. I. genetic and pathogenic diversity in Colletotrichum gloeosporioides isolates from Stylosanthes guianensis. 260 $c1997 520 $aAnthacnose, caused by Colletotrichum gloeosporioides, is the most important and widespread disease of Stylosanthes, a diverse tropical and subtropical forage legume naturally disributed in central and South America. This paper compares the genetic and pathogenic diversity of 45 isolates, which originated from S. guianensis genotypes. Published information on the pathogenic diversity of these isolates on a set of 12 S. guianensis was used. The amount of genetic diversity was measured at molecular level by polymerase chain reaction amplifications of DNA with 9 arbitrary primers of 10 bases each using the Random Amplified Polymorphic DNA (RAPD) ,markers. The amplifications revealed scorable polymorphism among the isolates, and a total of 80 band positions were scored. Using Ward s method (N igual 6) of statistical analysis, the isolates were separated into 6 clusters. Generally, isolates were clustered togheder by their geographic origin and/or their original host genotype. Isolates from Carimagua, Colombia, a savanna ecosystem and a long-time Stylosanthes breeding and selection site, exhibited a relatively wider range of genetic diversity than those from a newly opened trial site in the Amazon basin of Colombia. No strict correlation existed between genetic diversity, as measured by RAPD, and differential virulence, as defined by the pathotype. Isolates of the same pathotype/RAPD grouping often originated from the same host genotype and/or geographical locality. This information will be useful in determining future sampling strategies for the pathogen population. 650 $apathogenicity 650 $aBiodiversidade 650 $aColletotrichum Gloeosporioides 650 $aPatogenicidade 650 $aStylosanthes Guianensis 653 $aBiodversity 700 1 $aBADEL, J. L. 700 1 $aMORENO, C. X. 700 1 $aMILES, J. W. 700 1 $aCHAKRABORTY, S. 700 1 $aFERNANDES, C. D. 700 1 $aCHARCHAR, M. J. D' A. 773 $tTropical Grasslands, Queensland$gv. 31, p. 387-392, 1997.
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Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
31/03/2009 |
Data da última atualização: |
31/03/2009 |
Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
Autoria: |
LÉDO, A. da S.; RANGEL, M. S. A.; MACHADO, C. de A.; OLIVEIRA, L. F. M. |
Afiliação: |
Ana da Silva Lédo, Embrapa Tabuleiros Costeiros; Maria Salete Alves Rangel, Secretaria Executiva de Agricultura e Irrigação, Pesca e Abastecimento de Alagoas/Embrapa Tabuleiros Costeiros; Karla Cristina Santos Freire, (estagiário) Embrapa Tabuleiros Cost. |
Título: |
Propagação sexuada in vitro de moringa (Moringa oleifera Lam.). |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Aracaju: Embrapa Tabuleiros Costeiros, 2008. |
Páginas: |
18 p. |
Série: |
(Embrapa Tabuleiros Costeiros. Boletim de Pesquisa e Desenvolvimento, 39). |
Idioma: |
Português |
Palavras-Chave: |
Produção in vitro. |
Thesagro: |
Moringa. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00594nam a2200181 a 4500 001 1372707 005 2009-03-31 008 2008 bl uuuu u0uu1 u #d 100 1 $aLÉDO, A. da S. 245 $aPropagação sexuada in vitro de moringa (Moringa oleifera Lam.).$h[electronic resource] 260 $aAracaju: Embrapa Tabuleiros Costeiros$c2008 300 $a18 p. 490 $a(Embrapa Tabuleiros Costeiros. Boletim de Pesquisa e Desenvolvimento, 39). 650 $aMoringa 653 $aProdução in vitro 700 1 $aRANGEL, M. S. A. 700 1 $aMACHADO, C. de A. 700 1 $aOLIVEIRA, L. F. M.
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