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| 13. |  | OLIVEIRA, J. P. de; PEREIRA, J. E. S. Aclimatização de mudas de bananeira micropropagadas nas condições da Amazônia Ocidental. Horticultura Brasileira, v. 23, n. 2, 2005. Edição do CONGRESSSO BRASILEIRO DE OLERICULTURA, 45.; CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 15.; CONGRESSSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 2., 2005, Fortaleza.| Biblioteca(s): Embrapa Acre. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
29/03/2018 |
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Data da última atualização: |
29/03/2018 |
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Autoria: |
KAWANAMI, A. E.; OLIVEIRA, J. P. de; ARENALES, A.; CROSSLEY, B.; WOODS, L. W.; DUARTE, J. M. B.; WERTHER, K. |
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Afiliação: |
Aline E. Kawanami, Faculdade de Ciências Agrárias e Veterinárias - FCAV/Universidade Estadual Paulista “Júlio de Mesquita Filho” - Unesp; Juliana P. de Oliveira, Faculdade de Ciências Agrárias e Veterinárias - FCAV/Universidade Estadual Paulista “Júlio de Mesquita Filho” - Unesp; Alexandre Arenales, Faculdade de Ciências Agrárias e Veterinárias - FCAV/Universidade Estadual Paulista “Júlio de Mesquita Filho” - Unesp; Beate Crossley, California Animal Health and Food Safety Laboratory - CAHFS/University of California - UC; Leslie W. Woods, California Animal Health and Food Safety Laboratory - CAHFS/University of California - UC; José M. B. Duarte, Faculdade de Ciências Agrárias e Veterinárias - FCAV/Universidade Estadual Paulista “Júlio de Mesquita Filho” - Unesp; Karin Werther, Faculdade de Ciências Agrárias e Veterinárias - FCAV/Universidade Estadual Paulista “Júlio de Mesquita Filho” - Unesp. |
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Título: |
Detection of bluetongue virus in Brazilian cervids in São Paulo state. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 137-142, janeiro 2018. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Detecção de vírus da língua azul em cervídeos brasileiros no estado de São Paulo. |
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Conteúdo: |
Viral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion hemorrhages), it is necessary to use accurate techniques for a definitive etiologic diagnosis. Archival material (paraffin blocks) at the Department of Veterinary Pathology of FCAV ? Unesp was reviewed for lesions of hemorrhagic disease and 42 captive and free-living Brazilian deer were selected to include in this study. Paraffin-embedded tissues were evaluated using immunohistochemistry and tested negative for adenovirus. Using real time RT-PCR, EHD virus was not detected in paraffin-embedded tissues in any of the cases evaluated. The same technique was used for detection of BT virus and seven positive animals (16,66%) were confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in the positive animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Microscopic changes observed were lymphocytic inflammatory infiltrate in liver, kidney and lungs, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. This study demonstrates that the bluetongue virus is involved in hemorrhagic disease outbreaks of deer in Brazil. MenosViral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion hemorrhages), it is necessary to use accurate techniques for a definitive etiologic diagnosis. Archival material (paraffin blocks) at the Department of Veterinary Pathology of FCAV ? Unesp was reviewed for lesions of hemorrhagic disease and 42 captive and free-living Brazilian deer were selected to include in this study. Paraffin-embedded tissues were evaluated using immunohistochemistry and tested negative for adenovirus. Using real time RT-PCR, EHD virus was not detected in paraffin-embedded tissues in any of the cases evaluated. The same technique was used for detection of BT virus and seven positive animals (16,66%) were confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in the positive animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Microscopic changes observed were lymphocytic inflammatory infiltrate in liver, kidney and lungs, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. This study demonstrates that the b... Mostrar Tudo |
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Palavras-Chave: |
Adenoviral hemorrhagic disease; Brazilian cervids; Cervídeos brasileiros; Doença epizoótica hemorrágica; Doença hemorrágica por adenovírus; Hemorrhagic diseases. |
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Thesagro: |
Língua azul. |
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Thesaurus NAL: |
Bluetongue; Epizootic hemorrhagic disease; Viral diseases of animals and humans. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174735/1/Detection-of-bluetongue-virus-in-Brazilian.pdf
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Marc: |
LEADER 02710naa a2200325 a 4500
001 2089929
005 2018-03-29
008 2018 bl uuuu u00u1 u #d
100 1 $aKAWANAMI, A. E.
245 $aDetection of bluetongue virus in Brazilian cervids in São Paulo state.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Detecção de vírus da língua azul em cervídeos brasileiros no estado de São Paulo.
520 $aViral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion hemorrhages), it is necessary to use accurate techniques for a definitive etiologic diagnosis. Archival material (paraffin blocks) at the Department of Veterinary Pathology of FCAV ? Unesp was reviewed for lesions of hemorrhagic disease and 42 captive and free-living Brazilian deer were selected to include in this study. Paraffin-embedded tissues were evaluated using immunohistochemistry and tested negative for adenovirus. Using real time RT-PCR, EHD virus was not detected in paraffin-embedded tissues in any of the cases evaluated. The same technique was used for detection of BT virus and seven positive animals (16,66%) were confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in the positive animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Microscopic changes observed were lymphocytic inflammatory infiltrate in liver, kidney and lungs, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. This study demonstrates that the bluetongue virus is involved in hemorrhagic disease outbreaks of deer in Brazil.
650 $aBluetongue
650 $aEpizootic hemorrhagic disease
650 $aViral diseases of animals and humans
650 $aLíngua azul
653 $aAdenoviral hemorrhagic disease
653 $aBrazilian cervids
653 $aCervídeos brasileiros
653 $aDoença epizoótica hemorrágica
653 $aDoença hemorrágica por adenovírus
653 $aHemorrhagic diseases
700 1 $aOLIVEIRA, J. P. de
700 1 $aARENALES, A.
700 1 $aCROSSLEY, B.
700 1 $aWOODS, L. W.
700 1 $aDUARTE, J. M. B.
700 1 $aWERTHER, K.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 1, p. 137-142, janeiro 2018.
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