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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABREU, E. F. M.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
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Afiliação: |
Emanuel Felipe Medereiros Abreu, UnB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Molecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 34699 |
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Conteúdo: |
Cowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV. MenosCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively,... Mostrar Tudo |
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Palavras-Chave: |
CABMV; Cowpea; CpSMV; Molecular and RT-PCR analysis; Virus diseases. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02977nam a2200205 a 4500
001 1886945
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aABREU, E. F. M.
245 $aMolecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34699
520 $aCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV.
653 $aCABMV
653 $aCowpea
653 $aCpSMV
653 $aMolecular and RT-PCR analysis
653 $aVirus diseases
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Origem |
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Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
09/11/2012 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BATISTA, F. da S. L.; OLIVEIRA, J. B. de; VASCONCELOS, G. B. N.; BORGES, R. M. E.; BIASOTO, A. C. T.; PEREIRA, G. E. |
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Afiliação: |
FERNANDA DA SILVA LANTYER BATISTA; JULIANE BARRETO DE OLIVEIRA; GEISIANE BATISTA NUNES VASCONCELOS; RITA MERCIA ESTIGARRIBIA BORGES, CPATSA; ALINE CAMARAO TELLES BIASOTO, CPATSA; GIULIANO ELIAS PEREIRA, CNPUV / CPATSA. |
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Título: |
Influência de porta-enxertos sobre a composição do suco da uva Isabel Precoce elaborado no Vale do Submédio do São Francisco. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo desse trabalho foi avaliar a composição físico-química de sucos de uva elaborados a partir da variedade ‘Isabel Precoce’ (Vitis labrusca), enxertadas sobre diferentes porta-enxertos. |
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Palavras-Chave: |
Composição; Composição físico-química; Porta-enxerto; Suco de uva; Vale do São Francisco. |
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Thesagro: |
Qualidade; Uva; Vitis Vinifera. |
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Thesaurus NAL: |
Grapes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/69849/1/Giuliano5.pdf
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Marc: |
LEADER 01117nam a2200289 a 4500
001 1939373
005 2023-03-15
008 2012 bl uuuu u00u1 u #d
100 1 $aBATISTA, F. da S. L.
245 $aInfluência de porta-enxertos sobre a composição do suco da uva Isabel Precoce elaborado no Vale do Submédio do São Francisco.
260 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF$c2012
300 $c1 CD-ROM.
520 $aO objetivo desse trabalho foi avaliar a composição físico-química de sucos de uva elaborados a partir da variedade ‘Isabel Precoce’ (Vitis labrusca), enxertadas sobre diferentes porta-enxertos.
650 $aGrapes
650 $aQualidade
650 $aUva
650 $aVitis Vinifera
653 $aComposição
653 $aComposição físico-química
653 $aPorta-enxerto
653 $aSuco de uva
653 $aVale do São Francisco
700 1 $aOLIVEIRA, J. B. de
700 1 $aVASCONCELOS, G. B. N.
700 1 $aBORGES, R. M. E.
700 1 $aBIASOTO, A. C. T.
700 1 $aPEREIRA, G. E.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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