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| Registros recuperados : 315 | |
| Registros recuperados : 315 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
25/10/2017 |
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Data da última atualização: |
02/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BRAGA, L. F.; OLIVEIRA, F. A. de; COUTO, E. A. P. do; SANTOS, K. F. E. N.; FERREIRA, E. P. de B.; MARTIN-DIDONET, C. C. G. |
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Afiliação: |
LIVIA FABIANA BRAGA, UNIVERSIDADE ESTADUAL DE GOIÁS; FENIX ARAUJO DE OLIVEIRA, UNIVERSIDADE ESTADUAL DE GOIÁS; EVA APARECIDA PRADO DO COUTO, UNIVERSIDADE ESTADUAL DE GOIÁS; KARINA FREIRE d'EÇA NOGUEIRA SANTOS, UNIVERSIDADE ESTADUAL DE GOIÁS; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; CLAUDIA CRISTINA GARCIA MARTIN-DIDONET, UNIVERSIDADE ESTADUAL DE GOIÁS. |
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Título: |
Polyphasic characterization of bacteria obtainedfrom upland rice cultivated in Cerrado soil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 49, n. 1, p. 20-28, 2018. |
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ISSN: |
1678-4405 |
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DOI: |
10.1016/j.bjm.2017.04.004 |
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Idioma: |
Inglês |
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Conteúdo: |
This work aimed to characterize 20 isolates obtained from upland rice plants, based on phe-notypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90%of the isolates showed positive activity for amylase, catalase and, nitrogenase. All isolates were able to metabolize sucrose and malic acid in contrast with mannitol, which was metab-olized only by one isolate. For the other carbon sources, we observed a great variability inits use by the isolates. Most isolates showed antibiosis against Rhizoctonia solani (75%) and Sclerotinia sclerotiorum (55%) and, 50% of them showed antibiosis against both pathogens. Six isolates showed simultaneous ability of antibiosis, inorganic phosphate solubilization and protease activity. Based on phylogenetic analysis of the 16S rRNA gene all the isolates belong to Bacillus genus. Under greenhouse conditions, two isolates (S4 and S22) improved to about 24%, 25%, 30% and 31% the Total N, leaf area, shoot dry weight and root dry weight, respectively, of rice plants, indicating that they should be tested for this ability under field conditions. |
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Palavras-Chave: |
Associative bacteria; Bacterial diversity. |
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Thesagro: |
Arroz; Bactéria; Cerrado; Oryza sativa; Solo. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171165/1/CNPAF-2018-bjm.pdf
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Marc: |
LEADER 02233naa a2200289 a 4500
001 2078148
005 2018-05-02
008 2018 bl uuuu u00u1 u #d
022 $a1678-4405
024 7 $a10.1016/j.bjm.2017.04.004$2DOI
100 1 $aBRAGA, L. F.
245 $aPolyphasic characterization of bacteria obtainedfrom upland rice cultivated in Cerrado soil.$h[electronic resource]
260 $c2018
520 $aThis work aimed to characterize 20 isolates obtained from upland rice plants, based on phe-notypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90%of the isolates showed positive activity for amylase, catalase and, nitrogenase. All isolates were able to metabolize sucrose and malic acid in contrast with mannitol, which was metab-olized only by one isolate. For the other carbon sources, we observed a great variability inits use by the isolates. Most isolates showed antibiosis against Rhizoctonia solani (75%) and Sclerotinia sclerotiorum (55%) and, 50% of them showed antibiosis against both pathogens. Six isolates showed simultaneous ability of antibiosis, inorganic phosphate solubilization and protease activity. Based on phylogenetic analysis of the 16S rRNA gene all the isolates belong to Bacillus genus. Under greenhouse conditions, two isolates (S4 and S22) improved to about 24%, 25%, 30% and 31% the Total N, leaf area, shoot dry weight and root dry weight, respectively, of rice plants, indicating that they should be tested for this ability under field conditions.
650 $aArroz
650 $aBactéria
650 $aCerrado
650 $aOryza sativa
650 $aSolo
653 $aAssociative bacteria
653 $aBacterial diversity
700 1 $aOLIVEIRA, F. A. de
700 1 $aCOUTO, E. A. P. do
700 1 $aSANTOS, K. F. E. N.
700 1 $aFERREIRA, E. P. de B.
700 1 $aMARTIN-DIDONET, C. C. G.
773 $tBrazilian Journal of Microbiology$gv. 49, n. 1, p. 20-28, 2018.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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