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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
29/11/2020 |
Data da última atualização: |
29/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. |
Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
Título: |
Vertical transmissibility of small ruminant lentivirus. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
DOI: |
https://doi.org/10.1371/journal.pone.0239916 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. MenosAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in t... Mostrar Tudo |
Palavras-Chave: |
SRLV. |
Thesaurus Nal: |
Genetic techniques and protocols; Goat diseases; Goats; Lentivirus; Sheep diseases. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218347/1/CNPC-2020-Art-40.pdf
|
Marc: |
LEADER 02915naa a2200325 a 4500 001 2127175 005 2020-11-29 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1371/journal.pone.0239916$2DOI 100 1 $aARAÚJO, J. F. 245 $aVertical transmissibility of small ruminant lentivirus.$h[electronic resource] 260 $c2020 520 $aAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. 650 $aGenetic techniques and protocols 650 $aGoat diseases 650 $aGoats 650 $aLentivirus 650 $aSheep diseases 653 $aSRLV 700 1 $aANDRIOLI, A. 700 1 $aPINHEIRO, R. R. 700 1 $aSIDER, L. H. 700 1 $aSOUSA, A. L. M. de 700 1 $aAZEVEDO, D. A. A. de 700 1 $aPEIXOTO, R. M. 700 1 $aLIMA, A. M. C. 700 1 $aDAMASCENO, E. M. 700 1 $aSOUZA, S. C. R. 700 1 $aTEIXEIRA, M. F. da S. 773 $tPLoSONE$gv. 15, n. 11, e0239916, Nov. 2020.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
15/12/2020 |
Data da última atualização: |
15/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MARANGUAPE, J. S.; POMPEU, R. C. F. F.; VIEIRA, L. da S.; SOUZA, H. A. de; OLIVEIRA, E. L. de; SOUSA, A. M. P.; COSTA, C. dos S.; SANTOS, M. A. dos; PEREIRA, P. L.; SALLES, H. O. |
Afiliação: |
JÉSSICA SOUSA MARANGUAPE, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; LUIZ DA SILVA VIEIRA, CNPC; HENRIQUE ANTUNES DE SOUZA, CPAMN; EDUARDO LUIZ DE OLIVEIRA, CPPSE; ANA MÁRJORY PAIVA SOUSA, Rede Nordeste de Biotecnologia (RENORBIO), Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brasil; CLÉSIO DOS SANTOS COSTA, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brasil; MILENA ALVES DOS SANTOS, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brasil; PATRÍCIO LEANDRO PEREIRA, Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil; HEVILA OLIVEIRA SALLES, CNPC. |
Título: |
Castor cake as organic fertilizer to control gastrointestinal nematodes in pasture-raised sheep. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, v. 29, n. 4, e021420, Dec. 2020. |
DOI: |
https://doi.org/10.1590/s1984-29612020103. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Gastrointestinal parasitism is one of the factors that discourages farmers from raising small ruminants in cultivated pastures. To validate a soil treatment strategy to control the free-living stages of gastrointestinal nematodes (GIN), castor cake (CC) was used as a fertilizer on a pasture where sheep grazed on guinea grass under continuous stocking. On day zero, the pasture was divided into three paddocks, contaminated by GIN and treated, respectively, with CC divided into two applications (2CC1/2), CC in a single application (CC1) and organic compost in a single application (control). On day 21, eight GIN-free sheep were placed in each paddock. On day 58, significant differences (P<0.05) were observed: reduction of up to 66.10% in larvae.g-1 of dry mass in pastures fertilized with CC, decrease of up to 60.72% in infection rates among the animals in the groups treated with CC, higher average daily weight gain (over 185 g.day-1) and packed cell volume (over 26%) in the groups treated with CC, when compared to the control (128 g.day-1; 20.9%). In view of the results, the use of CC, mainly CC1, as a fertilizer for guinea grass pastures, under continuous stocking, proved to be promising, with 63.41% effectiveness in controlling worm infestations. Resumo: O parasitismo gastrintestinal é um dos fatores que fragiliza a exploração de pequenos ruminantes em pastagens cultivadas. Objetivando validar a estratégia de tratamento do solo para o controle dos estágios de vida livre de nematoides gastrintestinais (NGI), a torta de mamona (TM) foi utilizada como adubo, com ovinos pastejando em capim-tanzânia sob lotação contínua. No dia zero, o pasto foi dividido em três piquetes, contaminados por NGI e tratados, respectivamente, com TM parcelada em duas aplicações (2TM1/2), TM em uma única aplicação (TM1) e composto orgânico em única aplicação (testemunha). No dia 21, cada piquete recebeu oito ovinos livres de NGI. No dia 58, observaram-se diferenças significativas (P<0,05): redução de até 66,10% de larvas.g-1 de massa seca nas pastagens adubadas com TM; redução de até 60,72% da infecção dos animais nos grupos tratados com TM; ganho de peso médio diário (acima de 185 g.dia-1) e volume globular (acima de 26%) superior nos grupos tratados com TM, quando comparados à testemunha (128 g.dia-1; 20,9%). Diante dos resultados, o uso da TM, principalmente TM1, como adubo em pasto de capim-tanzânia, sob lotação contínua, mostrou-se promissor, com eficácia de 63,41% para controlar a verminose. MenosAbstract: Gastrointestinal parasitism is one of the factors that discourages farmers from raising small ruminants in cultivated pastures. To validate a soil treatment strategy to control the free-living stages of gastrointestinal nematodes (GIN), castor cake (CC) was used as a fertilizer on a pasture where sheep grazed on guinea grass under continuous stocking. On day zero, the pasture was divided into three paddocks, contaminated by GIN and treated, respectively, with CC divided into two applications (2CC1/2), CC in a single application (CC1) and organic compost in a single application (control). On day 21, eight GIN-free sheep were placed in each paddock. On day 58, significant differences (P<0.05) were observed: reduction of up to 66.10% in larvae.g-1 of dry mass in pastures fertilized with CC, decrease of up to 60.72% in infection rates among the animals in the groups treated with CC, higher average daily weight gain (over 185 g.day-1) and packed cell volume (over 26%) in the groups treated with CC, when compared to the control (128 g.day-1; 20.9%). In view of the results, the use of CC, mainly CC1, as a fertilizer for guinea grass pastures, under continuous stocking, proved to be promising, with 63.41% effectiveness in controlling worm infestations. Resumo: O parasitismo gastrintestinal é um dos fatores que fragiliza a exploração de pequenos ruminantes em pastagens cultivadas. Objetivando validar a estratégia de tratamento do solo para o controle dos estágios de vida li... Mostrar Tudo |
Palavras-Chave: |
Agroindustrial waste; Capim-tanzânia; Continuous stocking; Controle de verminose; Desempenho produtivo; Lotação contínua; Nematoides gastrintestinais; Resíduos agroindustriais; Torta de mamona. |
Thesagro: |
Eficiência Reprodutiva; Haemonchus Contortus; Helminto Gastrintestinal; Ovino; Resíduo Agrícola; Resíduo Industrial. |
Thesaurus NAL: |
Gastrointestinal nematodes; Megathyrsus maximus; Nematode control; Reproductive performance; Small ruminants. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219210/1/CNPC-2020-Art-55.pdf
|
Marc: |
LEADER 04081naa a2200481 a 4500 001 2128140 005 2020-12-15 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/s1984-29612020103.$2DOI 100 1 $aMARANGUAPE, J. S. 245 $aCastor cake as organic fertilizer to control gastrointestinal nematodes in pasture-raised sheep.$h[electronic resource] 260 $c2020 520 $aAbstract: Gastrointestinal parasitism is one of the factors that discourages farmers from raising small ruminants in cultivated pastures. To validate a soil treatment strategy to control the free-living stages of gastrointestinal nematodes (GIN), castor cake (CC) was used as a fertilizer on a pasture where sheep grazed on guinea grass under continuous stocking. On day zero, the pasture was divided into three paddocks, contaminated by GIN and treated, respectively, with CC divided into two applications (2CC1/2), CC in a single application (CC1) and organic compost in a single application (control). On day 21, eight GIN-free sheep were placed in each paddock. On day 58, significant differences (P<0.05) were observed: reduction of up to 66.10% in larvae.g-1 of dry mass in pastures fertilized with CC, decrease of up to 60.72% in infection rates among the animals in the groups treated with CC, higher average daily weight gain (over 185 g.day-1) and packed cell volume (over 26%) in the groups treated with CC, when compared to the control (128 g.day-1; 20.9%). In view of the results, the use of CC, mainly CC1, as a fertilizer for guinea grass pastures, under continuous stocking, proved to be promising, with 63.41% effectiveness in controlling worm infestations. Resumo: O parasitismo gastrintestinal é um dos fatores que fragiliza a exploração de pequenos ruminantes em pastagens cultivadas. Objetivando validar a estratégia de tratamento do solo para o controle dos estágios de vida livre de nematoides gastrintestinais (NGI), a torta de mamona (TM) foi utilizada como adubo, com ovinos pastejando em capim-tanzânia sob lotação contínua. No dia zero, o pasto foi dividido em três piquetes, contaminados por NGI e tratados, respectivamente, com TM parcelada em duas aplicações (2TM1/2), TM em uma única aplicação (TM1) e composto orgânico em única aplicação (testemunha). No dia 21, cada piquete recebeu oito ovinos livres de NGI. No dia 58, observaram-se diferenças significativas (P<0,05): redução de até 66,10% de larvas.g-1 de massa seca nas pastagens adubadas com TM; redução de até 60,72% da infecção dos animais nos grupos tratados com TM; ganho de peso médio diário (acima de 185 g.dia-1) e volume globular (acima de 26%) superior nos grupos tratados com TM, quando comparados à testemunha (128 g.dia-1; 20,9%). Diante dos resultados, o uso da TM, principalmente TM1, como adubo em pasto de capim-tanzânia, sob lotação contínua, mostrou-se promissor, com eficácia de 63,41% para controlar a verminose. 650 $aGastrointestinal nematodes 650 $aMegathyrsus maximus 650 $aNematode control 650 $aReproductive performance 650 $aSmall ruminants 650 $aEficiência Reprodutiva 650 $aHaemonchus Contortus 650 $aHelminto Gastrintestinal 650 $aOvino 650 $aResíduo Agrícola 650 $aResíduo Industrial 653 $aAgroindustrial waste 653 $aCapim-tanzânia 653 $aContinuous stocking 653 $aControle de verminose 653 $aDesempenho produtivo 653 $aLotação contínua 653 $aNematoides gastrintestinais 653 $aResíduos agroindustriais 653 $aTorta de mamona 700 1 $aPOMPEU, R. C. F. F. 700 1 $aVIEIRA, L. da S. 700 1 $aSOUZA, H. A. de 700 1 $aOLIVEIRA, E. L. de 700 1 $aSOUSA, A. M. P. 700 1 $aCOSTA, C. dos S. 700 1 $aSANTOS, M. A. dos 700 1 $aPEREIRA, P. L. 700 1 $aSALLES, H. O. 773 $tRevista Brasileira de Parasitologia Veterinária$gv. 29, n. 4, e021420, Dec. 2020.
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