Data/hora: 23/09/2024 12:11:26

Biblioteca(s): 	Embrapa Hortaliças.	
Data corrente: 	26/01/2015	
Data da última atualização: 	26/01/2015	
Autoria: 	HALLWASS, M.; OLIVEIRA, A. S. de; DIANESE, E. de C.; LOHUIS, D.; BOITEUX, L. S.; NAGATA, A. K. I.; RESENDE, R. O.; KORMELINK, R.	
Afiliação: 	MARIANA HALLWASS, LABORATORY OF VIROLOGY, DEPATMENT OF PLANT SCIENCES, WAGENINGEN UNIVERSITY.; ATHOS SILVA DE OLIVEIRA, INSTITUTODE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELUALAR, UNIVERSIDADE DE BRASILIA.; ERICO DE CAMPOS DIANESE, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; DICK LOHUIS, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; LEONARDO SILVA BOITEUX, CNPH; ALICE KAZUKO INOUE NAGATA, CNPH; RENATO O. RESENDE, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; RICHARD KORMELINK, LABORATORY OF VIROLOGY, DEPARTMENT OF PLANT SCIENCES, WAGENINGEN UNIVERSITY.	
Título: 	The tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Molecular Plant Pathology, v. 15, n. 9, p. 871-880, 2014.	
ISSN: 	1364-3703	
DOI: 	10.1111/mpp.12144	
Idioma: 	Inglês	
Conteúdo: 	Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum ?SR1? plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N. benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU).This is the first biological demonstration that Sw-5 -mediated resist-ance is triggered by the TSWV NSM cell-to-cell movement protein.	
Palavras-Chave: 	Avirulence; Cell-to-cell movement; Resistance gene; Sw-5; TSWV.	
Thesaurus Nal: 	Tospovirus.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Hortaliças (CNPH)	
Exemplares: 	CNPH - (39431-1) - UPC - AP - DD -  -  -  - DIS