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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
15/01/2013 |
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Data da última atualização: |
26/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, G. A. B. de; BATISTA, J. S. S.; MARCELINO, F. C.; HUNGRIA, M. |
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Afiliação: |
GESIELE ALMEIDA BARROS DE CARVALHO, UEL - UNIVERSIDADE ESTADUAL DE LONDRINA; JESIANE STEFÂNIA SILVA BATISTA; FRANCISMAR CORREA MARCELINO, CNPSO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Subtractive library of soybean roots in response to inoculation with Bradyrhizobium japonicum. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Biochemistry and Biotechnology Reports, Londrina, v.1, n.1 , p. 3-8, jan-jun 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Soybean [Glycine max (L.) Merr.] is the most important legume cropped worldwide, with an economic value attributed mainly to the high protein content of the grain, which, in turn, demands a heavy supply of nitrogen. An environmentally friendly source of nitrogen at low cost to farmers is represented by the biological fixation of atmospheric nitrogen that takes place is association with symbiotic bacteria. For the soybean, the association occurs mainly with bacteria belonging to the species Bradyrhizobium japonicum and Bradyrhizobium elkanii, but our knowledge about gene expression associated with the symbiosis is still poor. This work - part of an effort by the Soybean Genome Consortium (Genosoja)?was conducted with the aim of achieving better understanding about the functionality of the genes expressed in soybean roots in the early stages of the interaction with B. japonicum. The study was performed with soybean plants growing under greenhouse conditions, inoculated (or not) with strain CPAC 15 (=SEMIA 5079) of B. japonicum, which is broadly used in Brazilian commercial inoculants. Total RNA was extracted, the mRNA was isolated and a subtractive library was constructed. Sequencing analysis generated 4,621,072 reads, which were assembled in 3,776 sequences, defined as the differentially expressed sequences of the inoculated versus non-inoculated treatments. The sequences were categorized according to their molecular function and grouped with representatives of antioxidant activities, molecular transduction, transporters and enzyme regulation activities, but with an emphasis on catalytic and molecular binding/signaling. RESUMO: A soja [Glycine max (L.) Merr.] é uma leguminosa de grande expressão mundial, com valor econômico atribuído, principalmente, ao teor proteico elevado dos grãos, que, por sua vez, demandam altas doses de nitrogênio. Uma fonte de nitrogênio ambientalmente favorável e de baixo custo para os agricultores é representada pela fixação biológica do nitrogênio atmosférico, que ocorre através da associação com bactérias simbióticas. Na soja, essa associação ocorre, principalmente, com bactérias das espécies Bradyrhizobium japonicum e Bradyrhizobium elkanii, mas o conhecimento sobre a expressão gênica associada com a simbiose ainda é escasso. Este trabalho ? parte integrante do Consórcio do Genoma da Soja (Genosoja) ? foi conduzido com o objetivo de obter um melhor entendimento sobre a funcionalidade dos genes expressos nas raízes de soja nos estágios iniciais da interação com B. japonicum. O estudo foi conduzido com plantas de soja cultivadas sob condições de casa de vegetação, inoculadas (ou não) com a estirpe CPAC 15 (=SEMIA 5079) de B. japonicum, utilizada em larga escala em inoculantes comerciais no Brasil. O RNA foi extraídos das raízes, o mRNA foi isolado e a biblioteca subtrativa construída. O Sequenciamento gerou 4.621.072 leituras que, após a montagem resultaram em 3.776 sequências, definidas como diferencialmente expressas dos tratamentos inoculado versus não inoculado. As sequências foram agrupadas de acordo com sua função molecular, que resultou nas categorias de atividade antioxidante, transdução molecular, transporte, regulação de atividades enzimáticas, mas com ênfase nas atividades catalíticas e de ligação/ sinalização molecular. MenosABSTRACT: Soybean [Glycine max (L.) Merr.] is the most important legume cropped worldwide, with an economic value attributed mainly to the high protein content of the grain, which, in turn, demands a heavy supply of nitrogen. An environmentally friendly source of nitrogen at low cost to farmers is represented by the biological fixation of atmospheric nitrogen that takes place is association with symbiotic bacteria. For the soybean, the association occurs mainly with bacteria belonging to the species Bradyrhizobium japonicum and Bradyrhizobium elkanii, but our knowledge about gene expression associated with the symbiosis is still poor. This work - part of an effort by the Soybean Genome Consortium (Genosoja)?was conducted with the aim of achieving better understanding about the functionality of the genes expressed in soybean roots in the early stages of the interaction with B. japonicum. The study was performed with soybean plants growing under greenhouse conditions, inoculated (or not) with strain CPAC 15 (=SEMIA 5079) of B. japonicum, which is broadly used in Brazilian commercial inoculants. Total RNA was extracted, the mRNA was isolated and a subtractive library was constructed. Sequencing analysis generated 4,621,072 reads, which were assembled in 3,776 sequences, defined as the differentially expressed sequences of the inoculated versus non-inoculated treatments. The sequences were categorized according to their molecular function and grouped with representatives of anti... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio; Inoculação; Soja. |
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Thesaurus Nal: |
Nitrogen fixation; Root inoculation; Soybeans. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/74161/1/ID-34066.pdf
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Marc: |
LEADER 04093naa a2200229 a 4500
001 1945205
005 2017-07-26
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, G. A. B. de
245 $aSubtractive library of soybean roots in response to inoculation with Bradyrhizobium japonicum.$h[electronic resource]
260 $c2012
520 $aABSTRACT: Soybean [Glycine max (L.) Merr.] is the most important legume cropped worldwide, with an economic value attributed mainly to the high protein content of the grain, which, in turn, demands a heavy supply of nitrogen. An environmentally friendly source of nitrogen at low cost to farmers is represented by the biological fixation of atmospheric nitrogen that takes place is association with symbiotic bacteria. For the soybean, the association occurs mainly with bacteria belonging to the species Bradyrhizobium japonicum and Bradyrhizobium elkanii, but our knowledge about gene expression associated with the symbiosis is still poor. This work - part of an effort by the Soybean Genome Consortium (Genosoja)?was conducted with the aim of achieving better understanding about the functionality of the genes expressed in soybean roots in the early stages of the interaction with B. japonicum. The study was performed with soybean plants growing under greenhouse conditions, inoculated (or not) with strain CPAC 15 (=SEMIA 5079) of B. japonicum, which is broadly used in Brazilian commercial inoculants. Total RNA was extracted, the mRNA was isolated and a subtractive library was constructed. Sequencing analysis generated 4,621,072 reads, which were assembled in 3,776 sequences, defined as the differentially expressed sequences of the inoculated versus non-inoculated treatments. The sequences were categorized according to their molecular function and grouped with representatives of antioxidant activities, molecular transduction, transporters and enzyme regulation activities, but with an emphasis on catalytic and molecular binding/signaling. RESUMO: A soja [Glycine max (L.) Merr.] é uma leguminosa de grande expressão mundial, com valor econômico atribuído, principalmente, ao teor proteico elevado dos grãos, que, por sua vez, demandam altas doses de nitrogênio. Uma fonte de nitrogênio ambientalmente favorável e de baixo custo para os agricultores é representada pela fixação biológica do nitrogênio atmosférico, que ocorre através da associação com bactérias simbióticas. Na soja, essa associação ocorre, principalmente, com bactérias das espécies Bradyrhizobium japonicum e Bradyrhizobium elkanii, mas o conhecimento sobre a expressão gênica associada com a simbiose ainda é escasso. Este trabalho ? parte integrante do Consórcio do Genoma da Soja (Genosoja) ? foi conduzido com o objetivo de obter um melhor entendimento sobre a funcionalidade dos genes expressos nas raízes de soja nos estágios iniciais da interação com B. japonicum. O estudo foi conduzido com plantas de soja cultivadas sob condições de casa de vegetação, inoculadas (ou não) com a estirpe CPAC 15 (=SEMIA 5079) de B. japonicum, utilizada em larga escala em inoculantes comerciais no Brasil. O RNA foi extraídos das raízes, o mRNA foi isolado e a biblioteca subtrativa construída. O Sequenciamento gerou 4.621.072 leituras que, após a montagem resultaram em 3.776 sequências, definidas como diferencialmente expressas dos tratamentos inoculado versus não inoculado. As sequências foram agrupadas de acordo com sua função molecular, que resultou nas categorias de atividade antioxidante, transdução molecular, transporte, regulação de atividades enzimáticas, mas com ênfase nas atividades catalíticas e de ligação/ sinalização molecular.
650 $aNitrogen fixation
650 $aRoot inoculation
650 $aSoybeans
650 $aFixação de nitrogênio
650 $aInoculação
650 $aSoja
700 1 $aBATISTA, J. S. S.
700 1 $aMARCELINO, F. C.
700 1 $aHUNGRIA, M.
773 $tBiochemistry and Biotechnology Reports, Londrina$gv.1, n.1 , p. 3-8, jan-jun 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
26/01/2022 |
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Data da última atualização: |
26/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
MEDEIROS, A. C.; CAIXETA, E. T.; OLIVEIRA, A. C. B. de; SOUSA, T. V.; STOCK, V. de M.; CRUZ, C. D.; ZAMBOLIM, L.; PEREIRA, A. P. |
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Afiliação: |
ALEXSANDRA CORREIA MEDEIROS, UFV; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; ANTONIO CARLOS BAIÃO DE OLIVEIRA, EPAMIG; TIAGO VIEIRA SOUSA, UFTM; VINÍCIUS DE MOURA STOCK, UFV; COSME DAMIÃO CRUZ, UFV; LAÉRCIO ZAMBOLIM, UFV; ANTONIO ALVES PEREIRA, EPAMIG. |
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Título: |
Combining ability and molecular marker approach identified genetic resources to improve agronomic performance in coffea arabica breeding. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Frontiers in Sustainable Food Systems, v. 5, 705278, 2021. |
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DOI: |
https://doi.org/10.3389/fsufs.2021.705278 |
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Idioma: |
Inglês |
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Conteúdo: |
Plant breeding aims to develop cultivars with good agronomic traits through gene recombination and elite genotype selection. To support Coffea arabica breeding programs and assist parent selection, molecular characterization, genetic diversity (GD) analyses, and circulating diallel studies were strategically integrated to develop new cultivars. Molecular markers were used to assess the GD of 76 candidate parents and verify the crossing of potential F1 hybrids. Based on the complementary agronomic traits and genetic distance, eight elite parents were selected for circulating diallel analysis. The parents and 12 hybrids were evaluated based on 10 morpho-agronomic traits. For each trait, the effects of general and specific combining abilities, as well as the averages of the parents, hybrids, and predicted hybrids, were estimated. Crosses that maximize the genetic gains for the main agronomic traits of C. arabica were identified. Joint analysis of phenotypic and molecular data was used to estimate the correlation between molecular GD, phenotypic diversity (PD), phenotypic mean, and combining ability. The selection of parents that optimize the allele combination for the important traits of C. arabica is discussed in detail. |
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Thesaurus NAL: |
Coffea arabica var. arabica; Molecular genetics; Plant breeding. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230544/1/Combining-Ability-and-Molecular.pdf
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Marc: |
LEADER 02073naa a2200253 a 4500
001 2139358
005 2022-01-26
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fsufs.2021.705278$2DOI
100 1 $aMEDEIROS, A. C.
245 $aCombining ability and molecular marker approach identified genetic resources to improve agronomic performance in coffea arabica breeding.$h[electronic resource]
260 $c2021
520 $aPlant breeding aims to develop cultivars with good agronomic traits through gene recombination and elite genotype selection. To support Coffea arabica breeding programs and assist parent selection, molecular characterization, genetic diversity (GD) analyses, and circulating diallel studies were strategically integrated to develop new cultivars. Molecular markers were used to assess the GD of 76 candidate parents and verify the crossing of potential F1 hybrids. Based on the complementary agronomic traits and genetic distance, eight elite parents were selected for circulating diallel analysis. The parents and 12 hybrids were evaluated based on 10 morpho-agronomic traits. For each trait, the effects of general and specific combining abilities, as well as the averages of the parents, hybrids, and predicted hybrids, were estimated. Crosses that maximize the genetic gains for the main agronomic traits of C. arabica were identified. Joint analysis of phenotypic and molecular data was used to estimate the correlation between molecular GD, phenotypic diversity (PD), phenotypic mean, and combining ability. The selection of parents that optimize the allele combination for the important traits of C. arabica is discussed in detail.
650 $aCoffea arabica var. arabica
650 $aMolecular genetics
650 $aPlant breeding
700 1 $aCAIXETA, E. T.
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aSOUSA, T. V.
700 1 $aSTOCK, V. de M.
700 1 $aCRUZ, C. D.
700 1 $aZAMBOLIM, L.
700 1 $aPEREIRA, A. P.
773 $tFrontiers in Sustainable Food Systems$gv. 5, 705278, 2021.
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