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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Solos. |
Data corrente: |
05/11/2019 |
Data da última atualização: |
20/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, A. K; OLIVEIRA, G. de A. R; BASSINELLO, P. Z.; LANNA, A. C.; NOVOTNY, E. H.; LIÃO, L. M. |
Afiliação: |
ANDRESSA KUHNEN SILVA, UFG; GERLON DE ALMEIDA RIBEIRO OLIVEIRA, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; ETELVINO HENRIQUE NOVOTNY, CNPS; LUCIANO MORAIS LIÃO, UFG. |
Título: |
Evaluation of lipoxygenase activity in common beans by UV and NMR spectroscopy: proposal for a complementary technique for enzymatic studies. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Food Analytical Methods, v. 13, p. 35-43, 2020. |
DOI: |
10.1007/s12161-019-01499-3 |
Idioma: |
Inglês |
Conteúdo: |
Factors that reduce the quality of common bean during storage, including oxidative rancidity caused by lipoxygenase, have been extensively studied. In this context, this work evaluates lipoxygenase activity in five Carioca bean cultivars at different temperatures and storage times using ultraviolet (UV) and nuclear magnetic resonance (NMR) spectroscopies. UV absorption analyses demonstrated that the storage temperature of the grains influences the kinetic reaction rates during the enzymatic activity of lipoxygenase. Pinto beans and CNFC 10467 presented lower initial activity than the three BRS varieties but were more sensitive to high temperatures. BRS Estilo presented an anomalous behavior, with a strong reduction in activity at 15 and 21 °C until 72 days of storage. BRSMG Madrepérola and BRS Pontal presented stable enzymatic rates until 108 days of storage. A 26% reduction in fatty acid content, as a result of lipoxygenase action during storage, was observed both in solution-state and high resolution magic angle spinning (HR-MAS) NMR studies. These results highlight the importance of NMR in enzymatic kinetic reactions studies, complementing the results obtained by UV. |
Thesagro: |
Armazenamento; Atividade Enzimática; Enzima; Feijão; Phaseolus Vulgaris. |
Thesaurus Nal: |
Beans; Enzyme activity; Enzymes; Lipoxygenase; Nuclear magnetic resonance spectroscopy; Solutions; Storage conditions. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02205naa a2200337 a 4500 001 2113930 005 2020-04-20 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s12161-019-01499-3$2DOI 100 1 $aSILVA, A. K 245 $aEvaluation of lipoxygenase activity in common beans by UV and NMR spectroscopy$bproposal for a complementary technique for enzymatic studies.$h[electronic resource] 260 $c2020 520 $aFactors that reduce the quality of common bean during storage, including oxidative rancidity caused by lipoxygenase, have been extensively studied. In this context, this work evaluates lipoxygenase activity in five Carioca bean cultivars at different temperatures and storage times using ultraviolet (UV) and nuclear magnetic resonance (NMR) spectroscopies. UV absorption analyses demonstrated that the storage temperature of the grains influences the kinetic reaction rates during the enzymatic activity of lipoxygenase. Pinto beans and CNFC 10467 presented lower initial activity than the three BRS varieties but were more sensitive to high temperatures. BRS Estilo presented an anomalous behavior, with a strong reduction in activity at 15 and 21 °C until 72 days of storage. BRSMG Madrepérola and BRS Pontal presented stable enzymatic rates until 108 days of storage. A 26% reduction in fatty acid content, as a result of lipoxygenase action during storage, was observed both in solution-state and high resolution magic angle spinning (HR-MAS) NMR studies. These results highlight the importance of NMR in enzymatic kinetic reactions studies, complementing the results obtained by UV. 650 $aBeans 650 $aEnzyme activity 650 $aEnzymes 650 $aLipoxygenase 650 $aNuclear magnetic resonance spectroscopy 650 $aSolutions 650 $aStorage conditions 650 $aArmazenamento 650 $aAtividade Enzimática 650 $aEnzima 650 $aFeijão 650 $aPhaseolus Vulgaris 700 1 $aOLIVEIRA, G. de A. R 700 1 $aBASSINELLO, P. Z. 700 1 $aLANNA, A. C. 700 1 $aNOVOTNY, E. H. 700 1 $aLIÃO, L. M. 773 $tFood Analytical Methods$gv. 13, p. 35-43, 2020.
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Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
29/11/2022 |
Data da última atualização: |
29/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 4 |
Autoria: |
CANELLAS, L. P.; OLIVARES, F. L.; CANELLAS, N. O. A.; JINDO, K.; ROSA, R. C. C.; PICCOLO, A. |
Afiliação: |
LUCIANO PASQUALOTO CANELLAS, UENF; FÁBIO LOPES OLIVARES, UENF; NATÁLIA OLIVEIRA AGUIAR CANELLAS, UENF; KEIJI JINDO, WAGENINGEN UNIVERSITY; RAUL CASTRO CARRIELLO ROSA, CNPAB; ALESSANDRO PICCOLO UNIVERSITA DI NAPOLI, UNIVERSITA DI NAPOLI. |
Título: |
Challenge of transition: the history of a case study involving tropical fruits polyculture stimulated by humic acids and plant-growth promoting bacteria. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Chemical and Biological Technologies in Agriculture, v. 9, Article number 76, 2022. |
ISSN: |
2196-5641 |
Idioma: |
Inglês |
Conteúdo: |
Innovative technologies are required in agricultural production to eliminate the environmental risk generated by the intensive use of fertilizers and pesticides. Soil organic matter is a keystone for the transition towards sustainable production. However, it is not an easy task to increase soil organic matter in highly weathered soils without considerable resources and energy. Here, we highlighted the role of biological inputs in plant adaptation to low fertility and water scarcity. The direct use of humic acids and plant growth-promoting bacteria on plants can modify the root architecture systems, including surface area and roots length, thus allowing greater soil exploration. Material and methods: Within a socio-historical perspective of concepts and research methods, a case study is presented on the effects of humic acids applied together with plant-growth promoting bacteria, as an efficient tool for supporting the transition to more suitable production system. We implemented this natural ecological approach onto a polyculture system with different tropical fruits (banana, passion fruit, papaya and pineapple) and evaluated crop yields. Results: We observed increases of around 50 and 90% in banana and papaya yield, respectively, and 25% in passion fruit productions, with significantly greater yields maintained over four production cycles. No effect was observed in ananas production probably due to the large shading level in the area. Conclusion: The biostimulant formulated with endophytic diazotrophic bacteria and humic acids represents a low-cost technology that enhances crop yields and can play an important role in promoting a transition process towards sustainable agriculture. MenosInnovative technologies are required in agricultural production to eliminate the environmental risk generated by the intensive use of fertilizers and pesticides. Soil organic matter is a keystone for the transition towards sustainable production. However, it is not an easy task to increase soil organic matter in highly weathered soils without considerable resources and energy. Here, we highlighted the role of biological inputs in plant adaptation to low fertility and water scarcity. The direct use of humic acids and plant growth-promoting bacteria on plants can modify the root architecture systems, including surface area and roots length, thus allowing greater soil exploration. Material and methods: Within a socio-historical perspective of concepts and research methods, a case study is presented on the effects of humic acids applied together with plant-growth promoting bacteria, as an efficient tool for supporting the transition to more suitable production system. We implemented this natural ecological approach onto a polyculture system with different tropical fruits (banana, passion fruit, papaya and pineapple) and evaluated crop yields. Results: We observed increases of around 50 and 90% in banana and papaya yield, respectively, and 25% in passion fruit productions, with significantly greater yields maintained over four production cycles. No effect was observed in ananas production probably due to the large shading level in the area. Conclusion: The biostimulant formulated... Mostrar Tudo |
Palavras-Chave: |
Bioeconomy; Bioinputs; Ecofriendly practices; Microbial technologies. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02505naa a2200241 a 4500 001 2148934 005 2022-11-29 008 2022 bl uuuu u00u1 u #d 022 $a2196-5641 100 1 $aCANELLAS, L. P. 245 $aChallenge of transition$bthe history of a case study involving tropical fruits polyculture stimulated by humic acids and plant-growth promoting bacteria.$h[electronic resource] 260 $c2022 520 $aInnovative technologies are required in agricultural production to eliminate the environmental risk generated by the intensive use of fertilizers and pesticides. Soil organic matter is a keystone for the transition towards sustainable production. However, it is not an easy task to increase soil organic matter in highly weathered soils without considerable resources and energy. Here, we highlighted the role of biological inputs in plant adaptation to low fertility and water scarcity. The direct use of humic acids and plant growth-promoting bacteria on plants can modify the root architecture systems, including surface area and roots length, thus allowing greater soil exploration. Material and methods: Within a socio-historical perspective of concepts and research methods, a case study is presented on the effects of humic acids applied together with plant-growth promoting bacteria, as an efficient tool for supporting the transition to more suitable production system. We implemented this natural ecological approach onto a polyculture system with different tropical fruits (banana, passion fruit, papaya and pineapple) and evaluated crop yields. Results: We observed increases of around 50 and 90% in banana and papaya yield, respectively, and 25% in passion fruit productions, with significantly greater yields maintained over four production cycles. No effect was observed in ananas production probably due to the large shading level in the area. Conclusion: The biostimulant formulated with endophytic diazotrophic bacteria and humic acids represents a low-cost technology that enhances crop yields and can play an important role in promoting a transition process towards sustainable agriculture. 653 $aBioeconomy 653 $aBioinputs 653 $aEcofriendly practices 653 $aMicrobial technologies 700 1 $aOLIVARES, F. L. 700 1 $aCANELLAS, N. O. A. 700 1 $aJINDO, K. 700 1 $aROSA, R. C. C. 700 1 $aPICCOLO, A. 773 $tChemical and Biological Technologies in Agriculture$gv. 9, Article number 76, 2022.
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