Data/hora: 21/06/2024 00:38:25

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 25/04/1999
Data da última atualização: | 25/04/1999
Autoria: | REIS, V. M.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JUNIOR, F. B.; BALDANI, J. I.; DOBEREINER, Johanna.
Título: | Technical approaches to inoculate micropropagated sugar cane plants with Acetobacter diazotrophicus.
Ano de publicação: | 1999
Fonte/Imprenta: | Plant and Soil, Dordrecht, v. 206, p. 205-211, 1999.
Idioma: | Inglês
Conteúdo: | Micropropagated plantlets of sugar cane were inoculated with the N2-fixing bacterium Acetobacter diazotrophicus. Various modifications on the basic plant culture medium MS were made for the plant/bacteria association. The protocol required the inoculation of the bacteria at the end of the rooting period in a medium without hormones or vitamins, and with the concentration of sugar and mineral nutrients reduced by a factor of 10. Individual plants were inoculated with A. diazotrophicus and maintained under the appropriate light and temperature condition used for micropropagation up to 7 days. The system favored the infection and the establishment of the bacteria within the plant tissue. Bacteria colonized the plant tissue and accumulated in inter-cellular cavities and the region of lateral root emergence and also colonizes the xylem vessels. The inoculated plantlets were subsequently transferred to the aclimatization phase and after 30 days it was possible to isolate the bacteria from plant tissue. This protocol permitted studies of infection and comparison among strains.
Palavras-Chave: | Acetobacter diazotrophicus; BNF; Cana-de-açúcar; FBN; Fixação biologica de nitrogênio; In vitro; Nitrogen fixing bacteria; Sugar cane.
Thesagro: | Análise Estatística; Bactéria; Inoculação.
Thesaurus Nal: | inoculation methods; statistical analysis.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (24474-1) - UPC - SP - -- -  -  - 22313 - DIS