Data/hora: 21/06/2024 07:47:08

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 04/12/2013
Data da última atualização: | 21/09/2017
Autoria: | NUNES, R. L.; SANTOS, L. L. dos; BASTIANETTO, E.; OLIVEIRA, D. A. A. de; BRASIL, B. dos S. A. F.
Afiliação: | Ronaldo Luiz Nunes, UFMG; Livia Loiola dos Santos, UFMG; Eduardo Bastianetto, UFMG; Denise Aparecida Andrade de Oliveira, UFMG; BRUNO DOS SANTOS ALVES FIGUEIREDO BRASIL, CNPAE.
Título: | Frequency of benzimidazole resistance in Haemonchus contortus populations isolated from buffalo, goat and sheep herds.
Ano de publicação: | 2013
Fonte/Imprenta: | Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 22, n. 4, p. 1-6, 2013.
DOI: | 10.1590/S1984-29612013000400015.
Idioma: | Inglês
Conteúdo: | Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. Understanding of the genetic basis of benzimidazole resistance recently allowed the development of promising molecular diagnostic tools. In this study, isolates of Haemonchus contortus obtained from goats, sheep and buffaloes raised in Brazil were screened for presence of the polymorphism Phe200Tyr in the &#946; -tubulin 1 gene, which confers resistance to benzimidazole. The allelic frequency of the mutation conferring resistance ranged from 7% to 43%, and indicated that resistance to benzimidazole could be found in nematodes isolated from all the ruminant species surveyed. Although significant variation in the frequency of the F200Y mutation was observed between different herds or host species, no significant variation could be found in populations isolated from animals within the same herd. These findings suggest that screening of samples from a few animals has the potential to provide information about the benzimidazole resistance status of the entire herd, which would enable a considerable reduction in the costs of diagnosis for the producer. Molecular diagnosis has practical advantages, since it can guide the choice of anthelmintic drug that will be used, before its application in the herd, thus reducing the economic losses driven by anthelmintic resistance.
Palavras-Chave: | Benzimidazóis; Diagnóstico molecular; Polimorfismos; Resistência a drogas anti-helmíntico; Ruminantes domésticos.
Thesagro: | Genética.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (2319-1) - UPC - AP - DD -  -  -  - DIS