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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros; Embrapa Unidades Centrais. |
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Data corrente: |
27/10/2022 |
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Data da última atualização: |
26/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREIRE, G. da S.; ROCHA, L. B. da; MACHADO, C. de A.; SILVA, A. V. C. da; LEDO, A. da S. |
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Afiliação: |
GILMARA DA SILVA FREIRE; LETÍCIA BISPO DA ROCHA; CAROLINE DE ARAÚJO MACHADO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; ANA DA SILVA LEDO, CPATC. |
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Título: |
In vitro viability of genipap pollen grains in different culture media. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 57, e03020, 2022. |
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ISSN: |
1678-3921 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
ABSTRACT - The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-¹ H3BO3, 80 g L-¹ sucrose, and 1.0 g L-¹ agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains. RESUMO- O objetivo deste trabalho foi avaliar a viabilidade in vitro do grão de pólen de jenipapeiro (Genipa americana) à temperatura ambiente, em diferentes meios de cultura. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 3x11 (meios de cultura x tempos de incubação), com quatro repetições. O número de grãos de pólen germinados foi analisado em intervalos de 24 horas até 288 horas após incubação em temperatura ambiente. O meio com 100 mg L-¹ de H3BO3, 80 g L-¹ de sacarose e 1,0 g L-¹ de ágar resulta em alta à média percentagem de germinação in vitro, sendo o mais adequado para estudos da viabilidade in vitro de grãos de pólen de jenipapeiro. |
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Palavras-Chave: |
Germinação in vitro. |
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Thesagro: |
Genética Vegetal; Genipa Americana; Germinação; Jenipapo; Melhoramento Vegetal; Pólen; Tubo Polínico. |
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Thesaurus Nal: |
Culture media; Genipa; In vitro culture; Plant breeding; Pollen germination; Pollen tubes. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150239/1/In-vitro-viability-of-genipap-pollen....pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147847/1/In-vitro-viability-genipap-2022.pdf
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Marc: |
LEADER 02324naa a2200349 a 4500
001 2150239
005 2022-12-26
008 2022 bl uuuu u00u1 u #d
022 $a1678-3921
100 1 $aFREIRE, G. da S.
245 $aIn vitro viability of genipap pollen grains in different culture media.$h[electronic resource]
260 $c2022
520 $aABSTRACT - The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-¹ H3BO3, 80 g L-¹ sucrose, and 1.0 g L-¹ agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains. RESUMO- O objetivo deste trabalho foi avaliar a viabilidade in vitro do grão de pólen de jenipapeiro (Genipa americana) à temperatura ambiente, em diferentes meios de cultura. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 3x11 (meios de cultura x tempos de incubação), com quatro repetições. O número de grãos de pólen germinados foi analisado em intervalos de 24 horas até 288 horas após incubação em temperatura ambiente. O meio com 100 mg L-¹ de H3BO3, 80 g L-¹ de sacarose e 1,0 g L-¹ de ágar resulta em alta à média percentagem de germinação in vitro, sendo o mais adequado para estudos da viabilidade in vitro de grãos de pólen de jenipapeiro.
650 $aCulture media
650 $aGenipa
650 $aIn vitro culture
650 $aPlant breeding
650 $aPollen germination
650 $aPollen tubes
650 $aGenética Vegetal
650 $aGenipa Americana
650 $aGerminação
650 $aJenipapo
650 $aMelhoramento Vegetal
650 $aPólen
650 $aTubo Polínico
653 $aGerminação in vitro
700 1 $aROCHA, L. B. da
700 1 $aMACHADO, C. de A.
700 1 $aSILVA, A. V. C. da
700 1 $aLEDO, A. da S.
773 $tPesquisa Agropecuária Brasileira$gv. 57, e03020, 2022.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
15/01/2016 |
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Data da última atualização: |
06/09/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NUNES, J. F.; CORTEEL, J. M.; COMBARNOUS, Y.; BARIL, G. |
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Afiliação: |
Jose Ferreira Nunes, CNPC. |
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Título: |
Study of the involvement of seminal plasma constituents in the seasonal variations of goat spermatozoa motility. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON GOAT PRODUCTION AND DISEASE, 3., 1982, Tucson. Proceedings... Scottsdale: Dairy Goat Journal, 1982. p. 285. |
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Idioma: |
Inglês |
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Conteúdo: |
In previous studies we observed that the elimination of seminal plasma from ejaculated sperm before freezing was to obtain a good recovery of motility of spermatozoa (spz) after thawing. However, when the motility is tested immediately after ejaculation we find that seminal plasma obtained during the breeding season (BSSP), exhibits a positive effect on the maintenance of motility whether these spermatozoa are of breeding or of non-breeding season. In contrast, seminal plasma of non breeding season elicits a strongly negative effect. This negative effect is also observed when epididymal spermatozoa are used instead of ejaculated spermatozoa. Epididymal spermatozoa exhibit a much longer survival time than ejaculated spermatozoa in the absence of added material and, BSSP is without any effect. Thus the positive effect of BSSP on ejaculated spermatozoa is probably due to the inhibition of the negative factor from seminal plasma. Very likely, this negative factor acts rapidly explaining the shorter survival time of ejaculated spermatozoa compared to epididymal spermatozoa. This hypothesis is strengthened by the observation that a protein fraction from bulbourethral glands secretion exhibits a very strong negative effect on the motility of epididymal spermatozoa. The effect of this factor can be inhibited by material from the seminal vesicles. Thus variations of fertility of goat spermatozoa as a function of season might be controlled by the balance between these two factors. |
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Palavras-Chave: |
Motilidade; Movement; Preservação do sêmen; Semen preservation. |
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Thesagro: |
Caprino; Espermatozóide; Reprodução animal. |
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Thesaurus NAL: |
Goats; Reproduction; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02363nam a2200265 a 4500
001 2034033
005 2021-09-06
008 1982 bl uuuu u01u1 u #d
100 1 $aNUNES, J. F.
245 $aStudy of the involvement of seminal plasma constituents in the seasonal variations of goat spermatozoa motility.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON GOAT PRODUCTION AND DISEASE, 3., 1982, Tucson. Proceedings... Scottsdale: Dairy Goat Journal, 1982. p. 285.$c1982
520 $aIn previous studies we observed that the elimination of seminal plasma from ejaculated sperm before freezing was to obtain a good recovery of motility of spermatozoa (spz) after thawing. However, when the motility is tested immediately after ejaculation we find that seminal plasma obtained during the breeding season (BSSP), exhibits a positive effect on the maintenance of motility whether these spermatozoa are of breeding or of non-breeding season. In contrast, seminal plasma of non breeding season elicits a strongly negative effect. This negative effect is also observed when epididymal spermatozoa are used instead of ejaculated spermatozoa. Epididymal spermatozoa exhibit a much longer survival time than ejaculated spermatozoa in the absence of added material and, BSSP is without any effect. Thus the positive effect of BSSP on ejaculated spermatozoa is probably due to the inhibition of the negative factor from seminal plasma. Very likely, this negative factor acts rapidly explaining the shorter survival time of ejaculated spermatozoa compared to epididymal spermatozoa. This hypothesis is strengthened by the observation that a protein fraction from bulbourethral glands secretion exhibits a very strong negative effect on the motility of epididymal spermatozoa. The effect of this factor can be inhibited by material from the seminal vesicles. Thus variations of fertility of goat spermatozoa as a function of season might be controlled by the balance between these two factors.
650 $aGoats
650 $aReproduction
650 $aSpermatozoa
650 $aCaprino
650 $aEspermatozóide
650 $aReprodução animal
653 $aMotilidade
653 $aMovement
653 $aPreservação do sêmen
653 $aSemen preservation
700 1 $aCORTEEL, J. M.
700 1 $aCOMBARNOUS, Y.
700 1 $aBARIL, G.
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