Data/hora: 25/06/2024 12:43:44

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 08/10/2008
Data da última atualização: | 13/09/2021
Autoria: | FARIA, J. C. de; VALDISSER, P. A. M. R.; NOGUEIRA, E. O. P. e L.; ARAGÃO, F. J. L.
Afiliação: | JOSIAS CORREA DE FARIA, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; ELSA OLIVEIRA P E LAGO NOGUEIRA, Cenargen; FRANCISCO JOSE LIMA ARAGAO, Cenargen.
Título: | RNAi-based Bean golden mosaic virus-resistant common bean (Embrapa 5.1) shows simple inheritance for both transgene and disease resistance.
Ano de publicação: | 2014
Fonte/Imprenta: | Plant Breeding, v. 133, n. 5, p. 649-653, Oct. 2014.
ISSN: | 0179-9541
DOI: | https://doi.org/10.1111/pbr.12189
Idioma: | Inglês
Conteúdo: | Bean golden mosaic virus (BGMV) is the causal agent of bean golden mosaic of common beans. A transgenic bean line that has been developed based on RNA interference to silence the BGMV rep gene showed immunity to the virus. Crosses were done between the transgenic line and six bean cultivars followed by four backcrosses to the commercial cultivars ?Pérola? and ?BRS Pontal?. The transgene locus was consistently inherited from the crosses analysed in a Mendelian fashion in the segregating populations. The disease resistance reaction co-inherited with the transgene. Nevertheless, the expression of disease resistance displayed a dosage effect phenomenon in the F1 generation. The analysis of the homozygous near-isogenic lines in field conditions, under high BGMV disease incidence, indicated that the transgenic lines were completely resistant. These results show the strength of the disease resistance obtained, the stability of the trait across generations and its usefulness in the management of a disease for which there is no reported Phaseolus germplasm with immunity.
Palavras-Chave: | Common bean transformation; Genetic variability; Transgene stability; Virus resistance.
Thesagro: | Feijão; Organismo Transgênico; Phaseolus Vulgaris; Planta Transgênica; Variação Genética; Variedade Resistente; Vírus.
Thesaurus Nal: | Beans; Genetic variation; Genetically modified plants; Transgenes.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (27479-1) - UPC - AP - DD - 2014 -  - 2014 - DIS