Data/hora: 23/09/2024 02:42:38

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Data corrente: 	07/06/2004	
Data da última atualização: 	05/05/2022	
Autoria: 	VIEIRA, E. C.; NOGUEIRA, A. R. de A.	
Afiliação: 	EDIVAN CARVALHO VIEIRA, UFSCAR; ANA RITA DE ARAUJO NOGUEIRA, CPPSE.	
Título: 	Orthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	Journal of Agricultural and Food Chemistry, v.52, p.1800-1803, 2004.	
DOI: 	https://doi.org/10.1021/jf034467f	
Idioma: 	Inglês	
Conteúdo: 	A flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively.	
Palavras-Chave: 	Acid; Enzymatic hysrolysis; Immobilization; Phytase; Phytic; Spectophometry.	
Categoria do assunto: 	W Química e Física	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/88222/1/PROCI-2004.00041.pdf	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (15139-1) - UPC - AP - DD - PROCI-2004.00041 - VIE - 2004.00041 - DIS