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Registros recuperados : 202 | |
7. | | NICKEL, O. Doenças causadas por vírus em morangos, amoras pretas, framboesas e mirtilos. In.: SEMINÁRIO BRASILEIRO SOBRE PEQUENAS FRUTAS, 1., 2003, Bento Gonçalves, RS. Anais... Bento Gonçalves: SBPF, p. 41-47, 2004. (Embrapa Uva e vinho. Documentos, 37). Biblioteca(s): Embrapa Uva e Vinho. |
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Registros recuperados : 202 | |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
22/05/2014 |
Data da última atualização: |
02/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
NICKEL, O.; FAJARDO, T. V. M. |
Afiliação: |
OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV. |
Título: |
Detection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Journal of Plant Pathology, v. 96, n. 1, p. 207-213, 2014. |
Idioma: |
Inglês |
Conteúdo: |
Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs. |
Palavras-Chave: |
RT-PCR. |
Thesagro: |
DNA; Maça; Pera; Virus. |
Thesaurus NAL: |
Apple chlorotic leaf spot virus; Apple mosaic virus; Apple stem grooving virus; Apple stem pitting virus; Fruit quality; Reverse transcriptase polymerase chain reaction. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102459/1/JPP-2014-RT-PCR-Virus-macieira-Nickel-Thor.pdf
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Marc: |
LEADER 01818naa a2200265 a 4500 001 1986801 005 2019-04-02 008 2014 bl uuuu u00u1 u #d 100 1 $aNICKEL, O. 245 $aDetection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes.$h[electronic resource] 260 $c2014 520 $aApple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs. 650 $aApple chlorotic leaf spot virus 650 $aApple mosaic virus 650 $aApple stem grooving virus 650 $aApple stem pitting virus 650 $aFruit quality 650 $aReverse transcriptase polymerase chain reaction 650 $aDNA 650 $aMaça 650 $aPera 650 $aVirus 653 $aRT-PCR 700 1 $aFAJARDO, T. V. M. 773 $tJournal of Plant Pathology$gv. 96, n. 1, p. 207-213, 2014.
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Embrapa Uva e Vinho (CNPUV) |
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