| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Algodão. |
|
Data corrente: |
03/03/2017 |
|
Data da última atualização: |
03/03/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
OLIVEIRA, R. S. de; OLIVEIRA NETO, O. B.; MOURA, H. F. N.; MACEDO, L. L. P. de; ARRAES, F. B M.; LUCENA, W. A.; LOURENCO, I. T.; BARBOSA, A. de D.; SILVA, M. C. M. da; SA, M. F. G. de. |
|
Afiliação: |
RAQUEL S. de OLIVEIRA, CATHOLIC UNIVERSITY OF BRASILIA; OSMUNDO B. OLIVEIRA NETO, CENARGEN; HUDSON F. N. MOURA, CENARGEN; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; FABRÍCIO B. M. ARRAES, CENARGEN; WAGNER ALEXANDRE LUCENA, CNPA; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; AULUS A. de DEUS BARBOSA, CENARGEN; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
Transgenic cotton plants expressing Cry1la12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis). |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Frontiers in Plant Science, v. 7, Article 165, 2016. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter. |
|
Palavras-Chave: |
Genetic cotton transformation; Gosspypium hirsutum; Polen-tube pathway. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157003/1/Transgenic-cotton-plants-expressing.pdf
|
|
Marc: |
LEADER 01499naa a2200265 a 4500
001 2066050
005 2017-03-03
008 2016 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, R. S. de
245 $aTransgenic cotton plants expressing Cry1la12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis).$h[electronic resource]
260 $c2016
520 $aGossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter.
653 $aGenetic cotton transformation
653 $aGosspypium hirsutum
653 $aPolen-tube pathway
700 1 $aOLIVEIRA NETO, O. B.
700 1 $aMOURA, H. F. N.
700 1 $aMACEDO, L. L. P. de
700 1 $aARRAES, F. B M.
700 1 $aLUCENA, W. A.
700 1 $aLOURENCO, I. T.
700 1 $aBARBOSA, A. de D.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
773 $tFrontiers in Plant Science$gv. 7, Article 165, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Algodão (CNPA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
10/08/2018 |
|
Data da última atualização: |
24/09/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
|
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
|
Título: |
PCR-based genotyping of SNP markers in sheep. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.4, p.651-654, aug. 2018. |
|
ISSN: |
1573-4978 |
|
DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
|
Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
|
Thesagro: |
Marcador Molecular; Ovino. |
|
Thesaurus NAL: |
Genome. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02300naa a2200289 a 4500
001 2094070
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
022 $a1573-4978
024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource]
260 $c2018
520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.
650 $aGenome
650 $aMarcador Molecular
650 $aOvino
653 $aPCR RFLP
653 $aResistência nematódeo gastrintestinal
653 $aTetra primer ARMS PCR
700 1 $aCRUVINEL, G. G.
700 1 $aMORAES, C. V.
700 1 $aDONATONI, F. A. B.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv.45, n.4, p.651-654, aug. 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
