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| Registros recuperados : 173 | |
| 43. |  | OLIVEIRA, A. de L.; ROCHA, M. I. P.; MELLO, S. S.; BUZATTO, V. C.; NICIURA, S. C. M. Estabelecimento de cultura celular primária de mioblastos de bovinos com diferentes genótipos para um SNP no gene CAST: resultados preliminares. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 4., 2012, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2012. p. 36. (Embrapa Instrumentação. Documentos, 56).| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 46. | | BUZZATO, V. C.; OLIVEIRA, A. L. de; ROCHA, M. I. P.; MELLO, S. S. de; NICIURA, S. C. M. Efeitos de sistemas nanoestruturados sobre a expressão gênica em fibroblastos bovinos in vitro In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 5., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação , 2013. p. 11 (Embrapa Pecuária Sudeste. Documentos, 110). Editado por Ana Rita de Araújo Nogueira, Simone Cristina Méo Niciura| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 47. | | MELLO, S. C.; ROCHA, M. I. P.; GROMBONI, J. G. G.; VENERONI, G. B.; NICIURA, S. C. M. Descrição de novos polimorfismos do tipo SNP no gene da calpastatina em bovinos. In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá, SP. Resumos... Guarujá: Sociedade Brasileira de Genética, 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 50. | | NUCCI, A. DA SILVA; GIRALDELO, L. A.; SANTOS, I. B. DOS; ALEMÁN GAINZA, Y.; NICIURA, S. C. M. Padronização de co-cultivo in vitro de Haemonchus contortus com células abomasais ovinas. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 12., 2020, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2020. p.13. (Embrapa Instrumentação. Documentos, 71).| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 51. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. del; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kina sea ctivities in bovine cytoplasts obtained by chemical enucleation techniques. Reproduction Fertility Devevelopment, v. 26, n. 1, p. 130, 2013.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 52. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. DEL; LIMA, M. R. DE; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kinase activities in bovine cytoplasts obtained by chemical enucleation techniques. Reproduction Fertility and Development, v. 26, n. 1, p. 130, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Biblioteca(s): Embrapa Gado de Leite. |
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| 53. | | MELLO, S. S.; ROCHA, M. I. P.; GIGLIOTI, R.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; ESTEVES, S. N.; NICIURA, S. C. M. Association between P-glycoprotein gene polymorphisms and multidrug resistance in Haemonchus contortus. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., 2012, Brasília, DF. Anais... Brasília: Embrapa Estudos e Capacitação, 2012. p.119-120| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 54. | | LANDIM JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M. Análise da expressao gênica do VEGF (Vascular Endothelial Growth Factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, pelo método de RT-PCR semiquantitativo. ARS VETERINARIA, v. 22, n. 3, p. 217-222, 2006.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 55. | | FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 56. | | MORAES, C. V.; CRUVINEL, G. G.; SANTOS, I. B. dos; FIGUEIREDO, A.; COSTELLA, D. M.; OKINO, C. H.; NICIURA, S. C. M. Investigação da ocorrência de eventos epigenéticos em Haemonchus contortus e sua relação com a resistência ao anti-helmíntico monepantel. In: WORKSHOP DO PROGRAMA DE PÓS-GRADUAÇÃO EM GENÉTICA EVOLUTIVA E BIOLOGIA MOLECULAR, 1., 2019, São Carlos, SP. Anais... São Carlos: UFSCAR, 2019. p.71| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 57. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 58. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 59. | | NICIURA, S. C. M.; BENAVIDES, M. V.; OKINO, C. H.; IBELLI, A. M. G.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. Genome-wide association study for Haemonchus contortus resistance in Morada Nova sheep. Pathogens, v. 11, n. 8, aug. 2022, 939. 12 p.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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| 60. | | DEA, R. C. D.; CATOIA, V.; TIZIOTO, P. C.; ROCHA, M. I. P.; REGITANO, L. C. de A.; NICIURA, S. C. M. Genotipagem de SNPs em genes candidatos a características de interesse comercial em bovinos de corte com sondas de hidrólise. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 4., 2012, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2012. p. 32. (Embrapa Instrumentação. Documentos, 56).| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 173 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL. |
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Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, 2015. |
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DOI: |
https://doi.org/10.1017/S0967199414000537 |
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Idioma: |
Inglês |
|
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
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Thesaurus NAL: |
chromatin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal
W Química e Física |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1018303/1/Chemically-induced-enucleation-of-activated-bovine-oocytes.pdf
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Marc: |
LEADER 02607naa a2200277 a 4500
001 2018303
005 2024-02-09
008 2015 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199414000537$2DOI
100 1 $aSARAIVA, N. Z.
245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource]
260 $c2015
520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos.
650 $achromatin
653 $aBovine
653 $aChemically induced enucleation
653 $aMicrotubule
653 $aNuclear transfer
700 1 $aOLIVEIRA, C. S.
700 1 $aLEAL, C. L. V.
700 1 $aCALLADO, M. del
700 1 $aVANTINI, R.
700 1 $aMONTEIRO, F. M.
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 23, n. 6, p. 852-862, 2015.
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Embrapa Gado de Leite (CNPGL) |
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