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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
16/11/2018 |
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Data da última atualização: |
06/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CONCEIÇÃO, A. A.; SOARES NETO, C. B.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; MILLER, R. N. G.; MENDONCA, S. |
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Afiliação: |
APARECIDO ALMEIDA CONCEIÇÃO, Universidade Federal da Bahia; CLEMENTE BATISTA SOARES NETO, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; ROBERT NEIL GERARD MILLER, Universidade de Brasília; SIMONE MENDONCA, CNPAE. |
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Título: |
Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, v. 13, n. 5, e0196164, May 2018. |
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DOI: |
https://doi.org/10.1371/journal.pone.0196164 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus. MenosAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free ... Mostrar Tudo |
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Palavras-Chave: |
Human-serum albumin; Performance liquid-chromatography; Terpenoid aldehydes. |
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Thesaurus Nal: |
Biomass; Cottonseed cake; Gossypol; White-rot fungi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222356/1/pone.0196164.pdf
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Marc: |
LEADER 02441naa a2200277 a 4500
001 2099518
005 2021-04-06
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0196164$2DOI
100 1 $aCONCEIÇÃO, A. A.
245 $aDevelopment of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.$h[electronic resource]
260 $c2018
520 $aAbstract: Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 um). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 ug/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.
650 $aBiomass
650 $aCottonseed cake
650 $aGossypol
650 $aWhite-rot fungi
653 $aHuman-serum albumin
653 $aPerformance liquid-chromatography
653 $aTerpenoid aldehydes
700 1 $aSOARES NETO, C. B.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMILLER, R. N. G.
700 1 $aMENDONCA, S.
773 $tPlos One$gv. 13, n. 5, e0196164, May 2018.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
21/09/2021 |
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Data da última atualização: |
21/09/2021 |
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Autoria: |
CAMURÇA, D. A.; NEIVA, J. N. M.; PIMENTEL, J. C. M.; LOBO, R. N. B.; MELO, F. I. O.; VASCONCELOS, V. R. de. |
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Afiliação: |
DANIEL AGUIAR CAMURÇA, Pós-graduação - Universidade Federal do Ceará; JOSE NEUMAN MIRANDA NEIVA, Universidade Federal do Ceará; JOSE CARLOS MACHADO PIMENTEL, CNPAT; RAIMUNDO NONATO BRAGA LOBO, CNPC; FRANCISCO IVALDO OLIVEIRA MELO, Universidade Federal do Ceará; VÂNIA RODRIGUES DE VASCONCELOS, CNPC. |
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Título: |
Desempenho produtivo e consumo de nutrientes em ovinos alimentados com dietas à base de feno de gramíneas tropicais. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 37., 2000, Viçosa, MG. Resumos dos trabalhos apresentados. Viçosa, MG: Sociedade Brasileira de Zootecnia, 2000. |
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Páginas: |
3 f. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: O estudo foi realizado com o objetivo de avaliar o desempenho produtivo e consumo de matéria seca e nutrientes em ovinos confinados e alimentados com dietas à base de fenos das gramíneas: capim elefante, capim buffel,
capim milhã-roxa e capim urochloa. O ganho de peso foi semelhante (P>0,05) entre as dietas estudadas. O valor médio para ganho de peso foi 95 g/animal/dia. Não foram observadas diferenças nos consumos de matéria seca e fibra
detergente neutro entre as várias dietas estudadas. As dietas à base de feno de milhã-rôxa permitiram maior consumo de proteína bruta. Conclui-se que os fenos avaliados podem ser utiizados na alimentação de ovinos.
[Performance of sheep fed with diets based on hay of tropical grass].
Abstract: This study aimed to evaluate the performance and intake of nutrients on sheeps in feedlot and fed with diets based on hay of grass: elephant grass, buffel grass, milha-rôxa grass and urochloa grass. The weight gain were similar
(P<0,005) among the studied diets. Average values for weight gain were 95 g/animal/day. For the intake of dry matter and neutral detergent fiber no differences were verified. The diets based on hay of milhã-rôxa grass allowed higher intake of crude protein. We can conclude that the hay can be used for feeding sheep in feedlot. |
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Palavras-Chave: |
Capim milhã-roxa. |
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Thesagro: |
Alimento para animal; Capim Buffel; Capim Elefante; Capim Urochloa; Confinamento; Feno; Ganho de peso; Gramínea Forrageira; Nutrição animal; Ovino; Valor nutritivo. |
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Thesaurus NAL: |
Animal nutrition; Brazil; Nutritive value; Sheep; Weight gain. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02533nam a2200385 a 4500
001 2134620
005 2021-09-21
008 2000 bl uuuu u01u1 u #d
100 1 $aCAMURÇA, D. A.
245 $aDesempenho produtivo e consumo de nutrientes em ovinos alimentados com dietas à base de feno de gramíneas tropicais.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 37., 2000, Viçosa, MG. Resumos dos trabalhos apresentados. Viçosa, MG: Sociedade Brasileira de Zootecnia$c2000
300 $a3 f.
520 $aResumo: O estudo foi realizado com o objetivo de avaliar o desempenho produtivo e consumo de matéria seca e nutrientes em ovinos confinados e alimentados com dietas à base de fenos das gramíneas: capim elefante, capim buffel, capim milhã-roxa e capim urochloa. O ganho de peso foi semelhante (P>0,05) entre as dietas estudadas. O valor médio para ganho de peso foi 95 g/animal/dia. Não foram observadas diferenças nos consumos de matéria seca e fibra detergente neutro entre as várias dietas estudadas. As dietas à base de feno de milhã-rôxa permitiram maior consumo de proteína bruta. Conclui-se que os fenos avaliados podem ser utiizados na alimentação de ovinos. [Performance of sheep fed with diets based on hay of tropical grass]. Abstract: This study aimed to evaluate the performance and intake of nutrients on sheeps in feedlot and fed with diets based on hay of grass: elephant grass, buffel grass, milha-rôxa grass and urochloa grass. The weight gain were similar (P<0,005) among the studied diets. Average values for weight gain were 95 g/animal/day. For the intake of dry matter and neutral detergent fiber no differences were verified. The diets based on hay of milhã-rôxa grass allowed higher intake of crude protein. We can conclude that the hay can be used for feeding sheep in feedlot.
650 $aAnimal nutrition
650 $aBrazil
650 $aNutritive value
650 $aSheep
650 $aWeight gain
650 $aAlimento para animal
650 $aCapim Buffel
650 $aCapim Elefante
650 $aCapim Urochloa
650 $aConfinamento
650 $aFeno
650 $aGanho de peso
650 $aGramínea Forrageira
650 $aNutrição animal
650 $aOvino
650 $aValor nutritivo
653 $aCapim milhã-roxa
700 1 $aNEIVA, J. N. M.
700 1 $aPIMENTEL, J. C. M.
700 1 $aLOBO, R. N. B.
700 1 $aMELO, F. I. O.
700 1 $aVASCONCELOS, V. R. de
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Embrapa Agroindústria Tropical (CNPAT) |
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