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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
30/06/2010 |
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Data da última atualização: |
18/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. |
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Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP-JABOTICABAL; JORG PATRICK BURGSTALLER, UNIVERSTIY OF VETERINARY MEDICINE - VIENNA; FELIPE PERECIN, UNESP - JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP - JABOTICABAL; MARCOS ROBERTO CHIARATTI, USP - PIRASSUNUNGA; SIMONE CRISTINA MÉO, CPPSE; MATHIAS MULLER, UNIVERSITY OF VETERINARY MEDICINE - VIENNA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL - QUEBEC; FLÁVIO VIEIRA MEIRELLES, USP - PIRASSUNUNGA; RALF STEINBORN, UNIVERSITY OF VETERINARY MEDICINE - VIENNA. |
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Título: |
Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010. |
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DOI: |
https://doi.org/10.1095/biolreprod.109.080564 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype. |
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Palavras-Chave: |
Bovine; Developmental biology; Embryo; Gamete biology; Ooplasmic. |
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Thesagro: |
DNA. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28805/1/PROCISCMN2010.00002.pdf
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Marc: |
LEADER 02370naa a2200313 a 4500
001 1856388
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008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1095/biolreprod.109.080564$2DOI
100 1 $aFERREIRA, C. R.
245 $aPronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line.$h[electronic resource]
260 $c2010
520 $aOoplasmic transfer (01) has been used in basic mouse research for studying lhe segregation of mtDNA, as well as in human assisted reproduction for improving embryo development in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that lhe moderate amount of mitochondria introduced by OT is transmitted to lhe offspring's oocytes; e.g., modifies lhe germ fine. The danar mtDNA was detectable in 25% and 65% of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1% to 33.5% and from 0.4% to 15.5%, can be explained by random genetic drift in lhe female germ line. Centrifugation-mediated enrichment of mitochondria il1 lhe pole zone of lhe recipient zygote's ooplasm and its substitution by danar ooplasm led to elevated proportions of danar mtDNA in reconstructed zygotes compared with zygotes produced by standard OT (23.6% :!: 9.6% versus 12.1% :!: 4.5%; P <0.0001). We also characterized lhe proliferation of mitochondria from lhe OT parents-the recipient zygote (Bos primigenius taurus type) and lhe danar ooplasm (B. primigenius indicus type). Regression analysis performed for 57 tissue samples collected from lhe seven OT fetuses at different points during fetal development found a decreasing proportion of donor mt DNA (r2=0.78). This indicates a preferred proliferation of recipient of the OT recipeint expressinf a B. primigenius indicus phenotype.
650 $aDNA
653 $aBovine
653 $aDevelopmental biology
653 $aEmbryo
653 $aGamete biology
653 $aOoplasmic
700 1 $aBURGSTALLER, J. P.
700 1 $aPERECIN, F.
700 1 $aGARCIA, J. M.
700 1 $aCHIARATTI, M. R.
700 1 $aMÉO, S. C.
700 1 $aMULLER, M.
700 1 $aSMITH, L. C.
700 1 $aMEIRELLES, F. V.
700 1 $aSTEINBORN, R.
773 $tBiology of Reproduction$gv. 82, n. 03, p. 563-571, mar. 2010.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
27/11/2014 |
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Data da última atualização: |
12/12/2014 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NASCIMENTO, S. M. C. do; CARVALHO, E. de A.; ISHIDA, A. K. N.; SILVA, J. C. da; CARVALHO, K. B. de A.; CONCEIÇÃO, V. D. da. |
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Afiliação: |
SILVIA MARA COELHO DO NASCIMENTO, GRADUANDA UFRA; EUDES DE ARRUDA CARVALHO, CPATU; ALESSANDRA KEIKO NAKASONE ISHIDA, CPATU; JOSEANI CASTRO DA SILVA, DOUTORANDA UFAL; KENNY BONFIM DE ARRUDA CARVALHO, CPATU; VALÉRIA DIAS DA CONCEIÇÃO, GRADUANDA UFRA. |
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Título: |
Diversidade morfológica de colônias de Bacillus sp. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014. |
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Idioma: |
Português |
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Palavras-Chave: |
Bactérias; Promoção de crescimento. |
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Thesagro: |
Controle Biológico. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112528/1/ResumoCBRG-743.pdf
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Marc: |
LEADER 00670nam a2200193 a 4500
001 2001063
005 2014-12-12
008 2014 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, S. M. C. do
245 $aDiversidade morfológica de colônias de Bacillus sp.
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2014
650 $aControle Biológico
653 $aBactérias
653 $aPromoção de crescimento
700 1 $aCARVALHO, E. de A.
700 1 $aISHIDA, A. K. N.
700 1 $aSILVA, J. C. da
700 1 $aCARVALHO, K. B. de A.
700 1 $aCONCEIÇÃO, V. D. da
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Embrapa Amazônia Oriental (CPATU) |
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