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Registros recuperados : 22 | |
3. | | OLIVEIRA, A. P.; HEINEMANN, M. B.; CORTEZ, A.; NASCIMENTO, P. M. P. do; LEITE, R. C.; VIANA, J. H. M. Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows. Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.676-679, maio, 2016. Nota científica. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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6. | | NASCIMENTO, P. M. P.; SANTOS, G. B.; PENIDO, A. O.; FONSECA, J. F. da; LEITE, R. C. Identification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats. Animal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015. Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th... Biblioteca(s): Embrapa Caprinos e Ovinos. |
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7. | | NASCIMENTO, P. M. P.; OLIVEIRA, A. P.; DINIZ, J. P. C.; BICALHO, J. M.; FONSECA, J. F. da; LEITE, R. C. Identification of CAE virus by qPCR in embryos recovered from seropositive dairy goats. Animal Reproduction, v. 10, n. 3, p. 629, Jul./Sept. 2013. Annual Meeting of The Brazilian Embryo Technology Society - SBTE, 27., 2013, Praia do Forte, BA, Brasil. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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8. | | NASCIMENTO, P. M. P.; BRANDÃO, F. Z.; PEREIRA, P. F. V.; PONTELLO, V. R.; OLIVEIRA, A. P.; BRUSCHI, J. H.; FONSECA, J. F. da. Evaluation rates of ovulation and pregnancy in Toggemburg goats after hormonal treatment with synthetic progesterone 12, 9 and 6 days. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 242. ref. 346 . Biblioteca(s): Embrapa Caprinos e Ovinos. |
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9. | | NASCIMENTO, P. M. P.; BRANDÃO, F. Z.; PEREIRA, P. F. V.; PONTELLO, V. R.; OLIVEIRA, A. P.; BRUSKI, J. H.; FICHER NETO, A.; FONSECA, J. F. da. Avaliação de protocolos hormonais de seis, nove e doze dias sobre o estro, ovulação e fertilidade de cabras da raça Toggenburg em anestro. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 35.; ENCONTRO DE SAÚDE VETERINÁRIA DO CONESUL, 1.; CONGRESSO ESTADUAL DE MEDICINA VETERINÁRIA, 17.; CONGRESSO ESTADUAL DA ANCLIVEPA/RS, 3.; CONGRESSO DE MÉDICOS VETERINÁRIOS DO CONESUL, 5., 2008, Gramado. Anais... Gramado: Sociedade Brasileira de Medicina Veterinária, 2008. 5 f. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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10. | | NASCIMENTO, P. M. P. do; BRANDÃO, F. Z.; PEREIRA, P. F. V.; PONTELLO, V. R.; TAVARES, L. L.; OLIVEIRA, A. P.; BRUSCHI, J. H.; FONSECA, J. F. da. Production of teaser female goat by short-term protocol of exogenous androgen administration. In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23., 2008, Querétaro, México. Sustainable goat production: challenges and opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. p. 449. Abstract 362. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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11. | | NASCIMENTO, P. M. P.; BRANDÃO, F. Z.; PEREIRA, P. F. V.; OLIVEIRA, A. P.; VIANA, J. H. M.; FONSECA, J. da F. Preovulatory follicle dynamics in Toggenburg goats after treatment with intravaginal implants of progesterone for 6, 9 or 12 days. Animal Reproduction, v. 6, n. 1, p. 226, Jan./Mar. 2009. Edição dos Abstracts do II International Symposium on animal Biology or Reproduction, São Paulo, nov. 2008. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | | NASCIMENTO, P. M. P.; OLIVEIRA, A. P.; LEITE, R. C.; BRANDÃO, F. Z.; BRUSCHI, J. H.; SOUZA, J. M. G. de; FONSECA, J. F. da. Pregnancy rate in Toggenburg goats after short, medium or long-term protocols for induction of synchronized estrus after natural mating or artificial insemination in different seasons. Acta Scientiae Veterinariae, Porto Alegre, v. 38, supl. 2, p. s734, 2010. Abstract 91. Edição dos resumos da 25. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, PE, 2010. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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13. | | NASCIMENTO, P. M. P.; BRANDAO, F. Z.; OLIVEIRA, A. P.; OLIVEIRA, M. E. F.; VIANA, J. H. M.; BRUSCHI, J. H.; FONSECA, J. F. da. Sincronização de estro em cabras da raça Toggenburg após a utilização de protocolos hormonais de curta, média e longa duração. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 18., 2009, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2009. Biblioteca(s): Embrapa Gado de Leite. |
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14. | | NASCIMENTO, P. M. P.; BRANDÃO, F. Z.; OLIVEIRA, A. P.; OLIVEIRA, M. E. F.; VIANA, J. H. M.; BRUSCHI, J. H.; FONSECA, J. F. da. Sincronização de estro em cabras da raça Toggenburg após a utilização de protocolos hormonais de curta, média e longa duração. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 18., 2009, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2009. p. 400. 1 CD-ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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15. | | NASCIMENTO, P. M. P.; OLIVEIRA, A. P.; LEITE, R. C.; BRANDÃO, F. Z.; BRUSCHI, J. H.; SOUZA, J. M. G.; FONSECA, J. F. da. Taxa de gestação em cabras da raça Toggenburg após protocolos de curta, média ou longa duração para indução de estro sincronizado após monta natural ou inseminação artificial em diferentes estações. Acta Scientiae Veterinariae, v. 38, p. 367, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010. Biblioteca(s): Embrapa Gado de Leite. |
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16. | | NASCIMENTO, P. M. P.; OLIVEIRA, A. P.; BRANDÃO, F. Z.; MAIA, A. L. R. S.; PINTO, P. H. N.; OLIVEIRA, M. E. F.; BRUSCHI, J. H.; VIANA, J. H. M.; FONSECA, J. F. da. Dinoprost administration, period of ovulation, and ovulatory follicular size on days four to six of estrous cycle in ewes. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, p. 197, 2010. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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17. | | NASCIMENTO, P. M. P.; OLIVEIRA, A. P.; BRANDÃO, F. Z.; MAIA, A. L. R. S.; PINTO, P. H. N.; OLIVEIRA, M. E. F.; BRUSCHI, J. H.; VIANA, J. H. M.; FONSECA, J. F. da. Dinoprost administration, period of ovulation, and ovulatory follicular size on days four to six of estrous cycle in ewes. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010. Biblioteca(s): Embrapa Gado de Leite. |
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18. | | MAIA, A. L. R. S.; SOUZA, J. M. G.; MARTINS, F. H.; BRUSCHI, J. H.; VIANA, J. H. M.; BRANDÃO, F. Z.; NASCIMENTO, P. M. P.; TORRES, C. A. A.; FONSECA, J. F. Resposta a tratamento hormonal experimental em cabras leiteiras acometidas por hidrometra (resultados preliminares). In: MINAS LEITE. SUSTENTABILIDADE DA PRODUÇÃO DE LEITE NA AGRICULTURA FAMILIAR. 10. 2008, Anais. Juiz de Fora. Embrapa Gado de Leite, 2008. 1 p. 1 CD. Biblioteca(s): Embrapa Gado de Leite. |
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19. | | MAIA, A. L. R. S.; SOUZA, J. M. G.; MARTINS, F. H.; BRUSCHI, J. H.; VIANA, J. H. M.; BRANDÃO, F. Z.; NASCIMENTO, P. M. P.; TORRES, C. A. A.; FONSECA, J. DA F. Resposta a tratamento hormonal experimental em cabras leiteiras acometidas por hidrometra: resultados preliminares. In: MINAS LEITE, 10., 2008, Juiz de Fora, MG. Sustentabilidade da produção de leite na agricultura familiar: anais. Juiz de Fora: Embrapa Gado de Leite, 2008. 4 f. 1 CD-ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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20. | | OLIVEIRA, M. E. F.; SANTOS, I. C. C.; PIERONI, J. S. P.; FERREIRA, R. M.; CORDEIRO, M. F.; NASCIMENTO, P. M. P.; SOUZA, S. F.; FONSECA, J. F. da; VICENTE, W. R. R. Superovulatory response and embryo production influenced by the addition of LH and effect of the repeatability in Santa Inês sheep. Reproduction, Fertility and Development, v.21, n. 1, p. 171, 2009. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, San Diego, California, 2009. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 22 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
23/08/2017 |
Data da última atualização: |
16/05/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
NASCIMENTO, P. M. P.; SANTOS, G. B.; PENIDO, A. O.; FONSECA, J. F. da; LEITE, R. C. |
Afiliação: |
Universidade Federal de Minas Gerais (UFMG) -Belo Horizonte, MG, Brazil; Universidade Federal Fluminense (UFF); UFMG -Belo Horizonte, MG, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; UFMG -Belo Horizonte, MG, Brazil. |
Título: |
Identification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015. |
Idioma: |
Inglês |
Notas: |
Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. |
Conteúdo: |
Abstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthesis was performed from the viral genome with the use of M MLV enzyme according to protocol. The samples were submitted to nested PCR (Fieni, 2010,). The presence of viral and pro-viral genome in pellet (samples 2), but not in the pellet 1 or embryonic samples (zona pellucida and inner cell mass) was identified. Results shows viral replication could happen in the reproductive system from naturally infected goats, what have never been described before. However, embryos recovery from the flushing media did not show to be permissive to virus. This might indicate that the non cirurgical technique can provide virus dilution, since previous studies in embryos collected surgically revealed the presence of the virus pellet in 1, what did not happen in present work. MenosAbstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthes... Mostrar Tudo |
Palavras-Chave: |
Animal biotechnology; Artrite encefalite caprina; CAEV; Caprine arthritis encephalit virus; In vivo embryos. |
Thesagro: |
Biotecnologia; Caprino; Doença animal; Reprodução animal; Transferência de embrião; Transmissão de doença. |
Thesaurus NAL: |
Animal diseases; Disease transmission; Embryo transfer; Goat diseases; Goats. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162905/1/CNPC-2015-Identification-of-arthritis.pdf
|
Marc: |
LEADER 03618nam a2200361 a 4500 001 2074275 005 2022-05-16 008 2015 bl uuuu u00u1 u #d 100 1 $aNASCIMENTO, P. M. P. 245 $aIdentification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats.$h[electronic resource] 260 $aAnimal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015.$c2015 500 $aProceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. 520 $aAbstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthesis was performed from the viral genome with the use of M MLV enzyme according to protocol. The samples were submitted to nested PCR (Fieni, 2010,). The presence of viral and pro-viral genome in pellet (samples 2), but not in the pellet 1 or embryonic samples (zona pellucida and inner cell mass) was identified. Results shows viral replication could happen in the reproductive system from naturally infected goats, what have never been described before. However, embryos recovery from the flushing media did not show to be permissive to virus. This might indicate that the non cirurgical technique can provide virus dilution, since previous studies in embryos collected surgically revealed the presence of the virus pellet in 1, what did not happen in present work. 650 $aAnimal diseases 650 $aDisease transmission 650 $aEmbryo transfer 650 $aGoat diseases 650 $aGoats 650 $aBiotecnologia 650 $aCaprino 650 $aDoença animal 650 $aReprodução animal 650 $aTransferência de embrião 650 $aTransmissão de doença 653 $aAnimal biotechnology 653 $aArtrite encefalite caprina 653 $aCAEV 653 $aCaprine arthritis encephalit virus 653 $aIn vivo embryos 700 1 $aSANTOS, G. B. 700 1 $aPENIDO, A. O. 700 1 $aFONSECA, J. F. da 700 1 $aLEITE, R. C.
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