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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/10/2010 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. |
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Afiliação: |
AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS. |
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Título: |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. |
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DOI: |
10.1590/S1516-635X2010000200005 |
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Idioma: |
Inglês |
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Conteúdo: |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). MenosAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais bef... Mostrar Tudo |
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Thesagro: |
Genética; Virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32305/1/Molecular-studies.pdf
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Marc: |
LEADER 02461naa a2200313 a 4500
001 1865545
005 2018-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1590/S1516-635X2010000200005$2DOI
100 1 $aABREU, J. T.
245 $aMolecular studies of the brazilian infectious bronchitis virus isolates.$h[electronic resource]
260 $c2010
520 $aAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).
650 $aGenética
650 $aVirus
700 1 $aMOURÃO, M. M.
700 1 $aSANTOS, C. E.
700 1 $aVELOSO, C. J. M.
700 1 $aRESENDE, J. S.
700 1 $aFLATSCHART, R. B.
700 1 $aFOLGUERAS-FLATSCHAR, A. V.
700 1 $aJUNIOR, S. N.
700 1 $aSANTORO, M. M.
700 1 $aMENDES, A. C. R.
700 1 $aFRANCO, G. R.
700 1 $aSILVA, A.
700 1 $aCAMPOS, A. B.
700 1 $aFERNANDEZ, S.
773 $tRevista Brasileira de Ciência Avícola, Campinas$gv. 12, n. 2, p. 107-110, 2010.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
08/08/2016 |
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Data da última atualização: |
13/12/2016 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
OLIVEIRA, A. P.; HEINEMANN, M. B.; CORTEZ, A.; NASCIMENTO, P. M. P. do; LEITE, R. C.; VIANA, J. H. M. |
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Afiliação: |
ANDRE PENIDO OLIVEIRA, EPAMIG; MARCOS BRYAN HEINEMANN, UFMG; ADRIANA CORTEZ, Universidade Santo Amaro; PAULA MARIA PIRES DO NASCIMENTO, UFMG; ROMULO CERQUEIRA LEITE, UFMG; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Bovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.676-679, maio, 2016. |
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Idioma: |
Inglês |
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Notas: |
Nota científica. |
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Conteúdo: |
The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV?1) in follicular fluid and in cumulus?oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV?1 by COC or follicular fluid. |
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Palavras-Chave: |
DNA viral; Fluido folicular; Infecciosa bovina; Risco de transmissão; Transmission risk; Viral DNA; Vírus da rinotraqueíte. |
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Thesagro: |
Bos indicus. |
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Thesaurus NAL: |
Bovine herpesvirus 1; Follicular fluid; Zebu. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146164/1/Bovine-herpesvirus-type.pdf
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Marc: |
LEADER 01655naa a2200325 a 4500
001 2058460
005 2016-12-13
008 2016 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, A. P.
245 $aBovine herpesvirus type 1 in cumulus-oocyte complexes collected from naturally infected cows.
260 $c2016
500 $aNota científica.
520 $aThe objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV?1) in follicular fluid and in cumulus?oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV?1 by COC or follicular fluid.
650 $aBovine herpesvirus 1
650 $aFollicular fluid
650 $aZebu
650 $aBos indicus
653 $aDNA viral
653 $aFluido folicular
653 $aInfecciosa bovina
653 $aRisco de transmissão
653 $aTransmission risk
653 $aViral DNA
653 $aVírus da rinotraqueíte
700 1 $aHEINEMANN, M. B.
700 1 $aCORTEZ, A.
700 1 $aNASCIMENTO, P. M. P. do
700 1 $aLEITE, R. C.
700 1 $aVIANA, J. H. M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 51, n. 5, p.676-679, maio, 2016.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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