| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
|
Data corrente: |
28/04/2020 |
|
Data da última atualização: |
19/11/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BASSO, M. F.; LOURENCO-TESSUTTI, I. T.; BUSANELLO, C.; PINTO, C. E. M.; FREITAS, E. de O.; RIBEIRO, T. P.; ENGLER, J. de A.; OLIVEIRA, A. C. de; MORGANTE, C. V.; ALVES-FERREIRA, M.; GROSSI-DE-SA, M. F. |
|
Afiliação: |
MARCOS FERNANDO BASSO; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CARLOS BUSANELLO, UFPEL; CLIDIA EDUARDA MOREIRA PINTO, UNB; ELINEA DE OLIVEIRA FREITAS, UNB; JANICE DE ALMEIDA ENGLER, INRA/CNRS/UNS, FRANCE; ANTONIO COSTA DE OLIVEIRA, UFPEL; CAROLINA VIANNA MORGANTE, CPATSA; MARCIO ALVES-FERREIRA, UFRJ; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
Insights obtained using different modules of the cotton uceA1.7 promoter. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Planta, v. 251, n. 2, 2020. |
|
DOI: |
https://doi.org/10.1007/s00425-020-03348-8 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Transcriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops. MenosTranscriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development o... Mostrar Tudo |
|
Palavras-Chave: |
Cotton constitutive promoter; New biotechnological tools; Transcriptional core promoter; Transgenic crops. |
|
Thesagro: |
Algodão; Gene Marcador; Genética. |
|
Thesaurus Nal: |
Gene expression. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
LEADER 02743naa a2200349 a 4500
001 2123510
005 2020-11-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00425-020-03348-8$2DOI
100 1 $aBASSO, M. F.
245 $aInsights obtained using different modules of the cotton uceA1.7 promoter.$h[electronic resource]
260 $c2020
520 $aTranscriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops.
650 $aGene expression
650 $aAlgodão
650 $aGene Marcador
650 $aGenética
653 $aCotton constitutive promoter
653 $aNew biotechnological tools
653 $aTranscriptional core promoter
653 $aTransgenic crops
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aBUSANELLO, C.
700 1 $aPINTO, C. E. M.
700 1 $aFREITAS, E. de O.
700 1 $aRIBEIRO, T. P.
700 1 $aENGLER, J. de A.
700 1 $aOLIVEIRA, A. C. de
700 1 $aMORGANTE, C. V.
700 1 $aALVES-FERREIRA, M.
700 1 $aGROSSI-DE-SA, M. F.
773 $tPlanta$gv. 251, n. 2, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agricultura Digital. |
|
Data corrente: |
10/08/2009 |
|
Data da última atualização: |
15/01/2020 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
|
Autoria: |
NASCIMENTO, C. R.; GONÇALVES, R. R. V.; ZULLO JÚNIOR, J.; ROMANI, L. A. S. |
|
Afiliação: |
C. R. NASCIMENTO, FEAGRI/UNICAMP; R. R. V. GONÇALVES, FEAGRI/UNICAMP; J. ZULLO JÚNIOR, CEPAGRI/UNICAMP; LUCIANA ALVIM SANTOS ROMANI, CNPTIA. |
|
Título: |
Estimation of sugar cane productivity using a time series of AVHRR/NOAA-17 images and a phenology-spectral model. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: INTERNATIONAL WORKSHOP ON THE ANALYSIS OF MULTI-TEMPORAL REMOTE SENSING IMAGES, 5., 2009, Groton, Connecticut. Proceedings... Storrs: UConn, 2009. |
|
Páginas: |
p. 365-372. |
|
Idioma: |
Inglês |
|
Notas: |
MultiTemp 2009. |
|
Conteúdo: |
Despite the economic and social importance of agribusiness in Brazil, the accurate estimation of the production of its main agricultural crops, such as sugar cane, is still a big challenge to the Country. Thus this work has the objective to assess a methodology based on the use of a phenology-spectral model proposed by Pellegrino (2001) and a time series of NDVI values. The tests were performed for three crop seasons (2003/2004, 2004/2005 e 2005/2006) and four cities located in the State of Sao Paulo (Jaboticabal, Pitangueiras, Ribeirao Preto and Sertaozinho) that is the main producer of sugar cane in Brazil. Reference values for productivity were provided by the IBGE (National Institute of Geography and Statistics). The methodology can be used to support operational systems of crop monitoring and forecasting since the estimation errors were less than 5% and the anticipation was from 110 to 150 days before harvest. |
|
Palavras-Chave: |
Georreferenciamento; Image processing; Imagens AVHRR/NOAA-17; Phenology-spectral model; Processamento de imagens NDVI; Séries temporais de valores NDVI; Sistema NAVPro. |
|
Thesagro: |
Agricultura; Cana de açúcar; Sensoriamento remoto. |
|
Thesaurus NAL: |
Remote sensing; Sugarcane; Time series analysis. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02051nam a2200325 a 4500
001 1256613
005 2020-01-15
008 2009 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, C. R.
245 $aEstimation of sugar cane productivity using a time series of AVHRR/NOAA-17 images and a phenology-spectral model.$h[electronic resource]
260 $aIn: INTERNATIONAL WORKSHOP ON THE ANALYSIS OF MULTI-TEMPORAL REMOTE SENSING IMAGES, 5., 2009, Groton, Connecticut. Proceedings... Storrs: UConn$c2009
300 $ap. 365-372.
500 $aMultiTemp 2009.
520 $aDespite the economic and social importance of agribusiness in Brazil, the accurate estimation of the production of its main agricultural crops, such as sugar cane, is still a big challenge to the Country. Thus this work has the objective to assess a methodology based on the use of a phenology-spectral model proposed by Pellegrino (2001) and a time series of NDVI values. The tests were performed for three crop seasons (2003/2004, 2004/2005 e 2005/2006) and four cities located in the State of Sao Paulo (Jaboticabal, Pitangueiras, Ribeirao Preto and Sertaozinho) that is the main producer of sugar cane in Brazil. Reference values for productivity were provided by the IBGE (National Institute of Geography and Statistics). The methodology can be used to support operational systems of crop monitoring and forecasting since the estimation errors were less than 5% and the anticipation was from 110 to 150 days before harvest.
650 $aRemote sensing
650 $aSugarcane
650 $aTime series analysis
650 $aAgricultura
650 $aCana de açúcar
650 $aSensoriamento remoto
653 $aGeorreferenciamento
653 $aImage processing
653 $aImagens AVHRR/NOAA-17
653 $aPhenology-spectral model
653 $aProcessamento de imagens NDVI
653 $aSéries temporais de valores NDVI
653 $aSistema NAVPro
700 1 $aGONÇALVES, R. R. V.
700 1 $aZULLO JÚNIOR, J.
700 1 $aROMANI, L. A. S.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
