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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; DANIEL ANDREWS DE MOURA FERNANDES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; VIVIANE LOPES BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ANA BEATRIZ DA SILVA CARVALHO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Record, e1064, 2021. |
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DOI: |
https://doi.org/10.1002/vetr.1064 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis. MenosAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the eff... Mostrar Tudo |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02718naa a2200301 a 4500
001 2136205
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/vetr.1064$2DOI
100 1 $aSANTOS, J. D. R.
245 $aHormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression.$h[electronic resource]
260 $c2021
520 $aAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
650 $aSheep
700 1 $aBATISTA, R. I. T. P.
700 1 $aUNGERFELD, R.
700 1 $aTAIRA, A. R.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFERNANDES, D. A. de M.
700 1 $aBALARO, M. F. A.
700 1 $aCOSENTINO, I. O.
700 1 $aBRAIR, V. L.
700 1 $aPINTO, P. H. N.
700 1 $aCARVALHO, A. B. da S.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tVeterinary Record, e1064, 2021.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Origem |
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Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
06/02/2024 |
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Data da última atualização: |
16/02/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VARGAS, J. R.; SOUSA, B. M. C. de; SILVA, D. Y. M.; PETRONIO, M. S.; NAKASU, E. Y. T.; INOUE-NAGATA, A. K. |
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Afiliação: |
JONAS RAFAEL VARGAS; BÁRBARA MAVIE CAMARGO DE SOUSA; DORIAN YEST MELO SILVA, Universidade de Brasília.; MAICON SEGALLA PETRONIO, N & P Desenvolvimento, Pesquisa e Inovação; ERICH YUKIO TEMPEL NAKASU, CNPH; ALICE KAZUKO INOUE NAGATA, CNPH. |
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Título: |
Latest advances in the use of double stranded RNA as a tool for viral protection in tomato plants. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais 2023. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. |
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Páginas: |
p. 670 |
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Descrição Física: |
Resumo. |
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Idioma: |
Inglês |
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Conteúdo: |
Lately, great attention has been diverted to elucidating the mechanisms underlining plant gene silencing and the action of non-coding RNA in plants. Concomitantly, knowledge about the interactions between plants and pathogens has evolved greatly, bringing to the spotlight the importance of double-stranded RNA (dsRNA) as a tool to generate or improve resistance against plant pathogens. |
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Thesagro: |
RNA; Tomate; Vírus. |
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Thesaurus NAL: |
Chitosan; Plant hormones. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161709/1/RA-CNPH-06022024-INOUE-NAGATA-et-al.-p-672.pdf
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Marc: |
LEADER 01172nam a2200241 a 4500
001 2161709
005 2024-02-16
008 2023 bl uuuu u00u1 u #d
100 1 $aVARGAS, J. R.
245 $aLatest advances in the use of double stranded RNA as a tool for viral protection in tomato plants.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais 2023. Brasília, DF: Sociedade Brasileira de Fitopatologia$c2023
300 $ap. 670$cResumo.
520 $aLately, great attention has been diverted to elucidating the mechanisms underlining plant gene silencing and the action of non-coding RNA in plants. Concomitantly, knowledge about the interactions between plants and pathogens has evolved greatly, bringing to the spotlight the importance of double-stranded RNA (dsRNA) as a tool to generate or improve resistance against plant pathogens.
650 $aChitosan
650 $aPlant hormones
650 $aRNA
650 $aTomate
650 $aVírus
700 1 $aSOUSA, B. M. C. de
700 1 $aSILVA, D. Y. M.
700 1 $aPETRONIO, M. S.
700 1 $aNAKASU, E. Y. T.
700 1 $aINOUE-NAGATA, A. K.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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