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| Registros recuperados : 88 | |
| 61. |  | NASCIMENTO, E. S. do; LIMA, H. L. S.; BRIGIDA, A. I. S.; ANDRADE, F. K.; BORGES, M. de F.; MORAIS, J. P. S.; MUNIZ, C. R.; ROSA, M. de F. Mesquite (Prosopis juliflora (Sw.)) extract is an alternative nutrient source for bacterial cellulose production. Journal Biobased Materials and Bioenergy, v. 10, n. 1, p. 63-70, 2016.| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Agroindústria Tropical. |
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| 62. | | NASCIMENTO, M. A. do; SILVA, L. C. da; BARROSO, T. L. C. T.; ALEXANDRE, J. de B.; ALVES, C. R.; FURTADO, R. F.; BENEVIDES, S. D.; MUNIZ, C. R.; BARROS, M. E. S. Micropartículas de óleo de pequi obtidas por coacervação complexa com goma de cajueiro e gelatina. Fortaleza: Embrapa Agroindústria Tropical, 2019. (Embrapa Agroindústria Tropical. Comunicado técnico, 253)| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 63. | | MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; SOUSA, C. A. F. de; GUEDES, M. I. F.; VAN DER SCHOOR, R.; JALINK, H. Monitoring cashew seedlings during interactions with the fungus Lasiodiplodia theobromae using chlorophyll fluorescence imaging. Photosynthetica, v. 52, n. 4, p. 529-537, 2014.| Biblioteca(s): Embrapa Agroenergia; Embrapa Agroindústria Tropical. |
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| 64. | | FONSECA, W. L.; CARDOSO, J. E.; OOTANI, M. A.; BRASIL, S.O.S.; ARAUJO, F. S. A.; LIMA, C. S.; VIANA, F. M. P.; MUNIZ, C. R.; ANDRADE, I. L.; MARTINS, M. V. V. Morphological, molecular phylogenetic and pathogenic analyses of Erysiphe spp. causing powdery mildew on cashew plants in Brazil. Plant Pathology, Oxford, v. 68, n. 6, p. 1157-1164, Aug. 2019.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 65. | | DUARTE, E. B.; BORGES, M. de F.; SOUZA FILHO, M. de S. M. de; ANDRADE, F.; FEITOSA, J. P. A.; MUNIZ, C. R.; BRIGIDA, A. I. S.; MORAIS, J. P. S.; ROSA, M. de F. Obtenção de nanocompósitos de celulose bacteriana e hidroxiapatita a partir de resíduos agroindustriais. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO (REDE AGRONANO), 8., 2014, Juiz de Fora. Anais. Juiz de Fora: Embrapa Gado de Leite, 2014.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 66. | | MESQUITA, A. L. M.; MONTENEGRO, A. A. T.; MOTA, M. do S. C. de S.; RODRIGUES, S. M. M.; MUNIZ, C. R.; SILVA, G. A. DA; OLIVEIRA, E. M. C. Ocorrência de duas novas espécies de Liogenys em cajueiro: descrição, comportamento e sugestão de manejo. Fortaleza: Embrapa Agroindústria Tropical, 2023. (Embrapa Agroindústria Tropical. Comunicado técnico, 281). ODS 12.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 67. | | BRAGA, L. A. C.; RODRIGUES, M. J.; SOUZA FILHO, M. de S. M. de; AZEREDO, H. M. C. de; SILVA, E. de O.; MUNIZ, C. R.; OLIVEIRA, A. V. de; RIBEIRO, H. L. Filmes comestíveis de emulsões de polissacarídeos de algas com cera de carnaúba. Fortaleza: Embrapa Agroindústria Tropical, 2017. 21 p. (Embrapa Agroindústria Tropical. Boletim de Pesquisa e Desenvolvimento, 134).| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 68. | | MARTINS, M. V. V.; VIANA, F. M. P.; VIDAL NETO, F. das C.; SERRANO, L. A. L.; ARAUJO, F. S. A.; FONSECA, W. L.; OOTANI, M. A.; CARDOSO, J. E.; MUNIZ, C. R.; FREIRE, F. das C. O. Bacteriose do cajueiro-anão: situação atual e sintomas em diferentes órgãos da planta. Fortaleza: Embrapa Agroindústria Tropical, 2021. (Embrapa Agroindústria Tropical. Comunicado técnico, 272).| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 69. | | LIMA, H. L. S.; NASCIMENTO, E. S.; ANDRADE, F. K.; BRIGIDA, A. I. S.; BORGES, M. de F.; CASSALES, A. R.; MUNIZ, C. R.; SOUZA FILHO, M. de S. M. de; MORAIS, J. P. S.; ROSA, M. de F. Bacterial cellulose production by Komagataeibacter hansenii ATCC 23769 using sisal juice: an agroindustry waste. Brazilian Journal of Chemical Engineering, v. 34, n. 3, p. 671-680, Jul./Sep. 2017.| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Agroindústria Tropical; Embrapa Algodão. |
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| 70. | | OLIVEIRA, J. M. DE; DIAS, N. da S.; MELO, J. W. DA S.; SARAIVA, W. V. A.; MUNIZ, C. R.; COSTA-LIMA, T. C. da; OLIVEIRA, V. R.; MACIEL, G. P. DE S. Leaf morphology of melon mediates feeding and oviposition preference, and immature survival of Liriomyza sativae (Blanchard) (Diptera: Agromyzidae). Phytoparasitica, fev., 2021.| Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Hortaliças; Embrapa Semiárido. |
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| 71. | | MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants. Annals of Applied Biology, v. 160, n. 3, p. 217-224, 2012.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 72. | | MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasioplodia theobromae, causal agent of gummosis in cashew nut plants. Annals of Applied Biology, v.160, p. 217-224, 2012.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 73. | | RODRIGUES, T. H. S.; PEREIRA, R. de C. A.; SILVA, L. M. A. e; AZEREDO, H. M. C. de; MUNIZ, C. R.; BRITO, E. S. de; CANUTO, K. M.; PINTO, N. de O. F.; CÁCERES, C. A. Production and physico-chemical characterization of nanocapsules of the essential oil from Lippia sidoides Cham. Industrial Crops and Products, v. 86, p. 279-288, 2016.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 74. | | DUARTE, E. B.; CHAGAS, B. S. DAS; ANDRADE, F. K.; BRIGIDA, A. I. S.; BORGES, M. de F.; MUNIZ, C. R.; SOUZA FILHO, M. de S. M. de; MORAIS, J. P. S.; FEITOSA, J. P. A.; ROSA, M. de F. Production of hydroxyapatite-bacterial cellulose nanocomposites from agroindustrial wastes. Cellulose, v. 22, n. 5, p. 3177-3187, 2015.| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Agroindústria Tropical. |
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| 75. | | OLIVEIRA, S. T.; AZEVEDO, M. I. G.; CUNHA, R. M. S.; SILVA, C. de F. B. da; MUNIZ, C. R.; MONTEIRO JÚNIOR, J. E.; CARNEIRO, R. F.; NAGANO, C. S.; GIRÃO, M. S.; FREITAS, C. D. T.; GRANGEIRO, T. B. Structural and functional features of a class VI chitinase from cashew (Anacardium occidentale L.) with antifungalproperties. Phytochemistry, v. 180, 112527, dezembro, 2020.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 76. | | COSTA, J. N. da; LEAL, A. R.; NASCIMENTO, L. G. L.; RODRIGUES, D. C.; MUNIZ, C. R.; FIGUEIREDO, R. W.; MATA, P.; NORONHA, J. P.; SOUSA, P. H. M. de. Texture, microstructure and volatile profile of structured guava using agar and gellan gum. International Journal of Gastronomy and Food Science, 20, art. no. 100207, 2020.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 77. | | LUZ, E. P. C. G.; CHAGAS, B. S. DAS; ALMEIDA, N. T. de; BORGES, M. DE F.; ANDRADE, F. K.; MUNIZ, C. R.; CASTRO-SILVA, I. I.; TEIXEIRA, E. H.; POPAT, K.; ROSA, M. de F.; VIEIRA, R. S. Resorbable bacterial cellulose membranes with strontium release for guided bone regeneration. Materials Science & Engineering. C, Biomimetic Materials, Sensors and Systems, v. 116, art. no. 111175, 2020.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 78. | | AZEVEDO, M. I. G.; OLIVEIRA, S. T.; SILVA, C. F. B.; CARNEIRO, R. F.; NAGANO, C. S.; GADELHA, A. C. S.; TORRES, D. C.; GIRÃO; MUNIZ, C. R.; FREITAS, C. D. T.; GRANGEIRO, T. B. Secretory production in Escherichia coli of a GH46 chitosanase from Chromobacterium violaceum, suitable to generate antifungal chitooligosaccharides. International Journal of Biological Macromolecules, v. 165, Part A, 15 December, p. 1482-1495, 2020.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 79. | | DUARTE, E. B; CHAGAS, B. S. das; ANDRADE, F. K.; BRIGIDA, A. I. S.; BORGES, M. de F.; MUNIZ, C. R.; SOUZA FILHO, M. de S. M. de; MORAIS, J. P. S.; FEITOSA, J. P. A.; ROSA, M. de F. Utilização de resíduos agroindustriais na elaboração de bionanocompósitos baseados em celulose bacteriana e Hidroxiapatita. In: CONGRESSO BRASILEIRO DE POLÍMEROS, 13., 2015, Natal. Anais... São Carlos: ABPol, 2015. 5 p.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 80. | | DUARTE, E. B; CHAGAS, B. S. das; ANDRADE, F. K.; BRIGIDA, A. I. S.; BORGES, M. de F.; MUNIZ, C. R.; SOUZA FILHO, M. de S. M. de; MORAIS, J. P. S.; FEITOSA, J. P. A.; ROSA, M. de F. Utilização de resíduos agroindustriais na elaboração de bionanocompósitos baseados em celulose bacteriana e Hidroxiapatita. In: CONGRESSO BRASILEIRO DE POLÍMEROS, 13., 2015, Natal. [São Carlos: Associação Brasileira de Polímeros], 2015. 5 p. SP2.05.48. AK4X.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 88 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
23/01/2013 |
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Data da última atualização: |
25/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. |
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Afiliação: |
CELLI RODRIGUES MUNIZ, CNPAT; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; FRANCISCO MARTO PINTO VIANA, CNPAT; JOSE EMILSON CARDOSO, CNPAT; DIVA CORREIA, CNPAT; WAGENINGEN UNIVERSITY GREENHOUSE HORTICULTURE; PLANT RESEARCH INTERNATIONAL; GILVAN FERREIRA DA SILVA, CPAA; UNIVERSIDADE ESTADUAL DO CEARÁ. |
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Título: |
Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 160, n. 3, p. 217-224, 2012. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/j.1744-7348.2012.00534.x |
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Idioma: |
Português |
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Conteúdo: |
Members of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity. MenosMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be dete... Mostrar Tudo |
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Palavras-Chave: |
Anacardium occidentale L; Cashew agri-industry; Endophytic fungi; Immunodetection. |
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Categoria do assunto: |
-- |
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Marc: |
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245 $aPolyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants.
260 $c2012
520 $aMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity.
653 $aAnacardium occidentale L
653 $aCashew agri-industry
653 $aEndophytic fungi
653 $aImmunodetection
700 1 $aFREIRE, F. das C. O.
700 1 $aVIANA, F. M. P.
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700 1 $aCORREIA, D.
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700 1 $aSILVA, G. F. da
700 1 $aGUEDES, M. I. F.
773 $tAnnals of Applied Biology$gv. 160, n. 3, p. 217-224, 2012.
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