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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
27/10/2015 |
Data da última atualização: |
18/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; SOUZA, A. da S.; LEDO, C. A. da S. |
Afiliação: |
Á. M. VIDAL, UFRB; L. J. VIEIRA; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
Título: |
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Genetics and Molecular Research, v.14, n. 3, p. 7759-7770, 2015. |
ISSN: |
1676-5680 |
Idioma: |
Inglês |
Conteúdo: |
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system. MenosMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava p... Mostrar Tudo |
Palavras-Chave: |
Marcado molecular. |
Thesagro: |
Anatomia vegetal; Banco de germoplasma; Mandioca; Meristema. |
Thesaurus Nal: |
Cassava; Genetic markers; Germplasm. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02450naa a2200289 a 4500 001 2027402 005 2023-05-18 008 2015 bl uuuu u00u1 u #d 022 $a1676-5680 100 1 $aVIDAL, Á. M. 245 $aGenetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.$h[electronic resource] 260 $c2015 520 $aMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system. 650 $aCassava 650 $aGenetic markers 650 $aGermplasm 650 $aAnatomia vegetal 650 $aBanco de germoplasma 650 $aMandioca 650 $aMeristema 653 $aMarcado molecular 700 1 $aVIEIRA, L. J. 700 1 $aFERREIRA, C. F. 700 1 $aSOUZA, F. V. D. 700 1 $aSOUZA, A. da S. 700 1 $aLEDO, C. A. da S. 773 $tGenetics and Molecular Research$gv.14, n. 3, p. 7759-7770, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
15/09/2009 |
Data da última atualização: |
13/11/2009 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MULLER, J. A.; CARGNIN, A.; MELO, F. D. A.; FOGAÇA, C. M. |
Afiliação: |
João Augusto Muller, estagiário CPAC; Adeliano Cargnin, CPAC; Fernando Daminelli Araújo melo, estagiário CPAC; Claúdia Martellet Fogaça, bolsista CPAC. |
Título: |
Sólidos solúveis em cultivares de cana-de-açúcar em condições de cerrado. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia: livro de resumos. Fortaleza: Sociedade Brasileira de Fisiologia Vegetal: UFC: Embrapa Agroindustria Tropical, 2009. p. 12. |
Páginas: |
p. 12. |
Idioma: |
Português |
Palavras-Chave: |
Cana-de-açúcar; Cultivo; Sccharum officinarum. |
Thesagro: |
Cerrado. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00761naa a2200205 a 4500 001 1572323 005 2009-11-13 008 2009 bl uuuu u00u1 u #d 100 1 $aMULLER, J. A. 245 $aSólidos solúveis em cultivares de cana-de-açúcar em condições de cerrado. 260 $c2009 300 $ap. 12. 650 $aCerrado 653 $aCana-de-açúcar 653 $aCultivo 653 $aSccharum officinarum 700 1 $aCARGNIN, A. 700 1 $aMELO, F. D. A. 700 1 $aFOGAÇA, C. M. 773 $tIn: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 12., 2009, Fortaleza. Desafios para produção de alimentos e bioenergia: livro de resumos. Fortaleza: Sociedade Brasileira de Fisiologia Vegetal: UFC: Embrapa Agroindustria Tropical, 2009. p. 12.
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