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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
28/01/1998 |
Data da última atualização: |
28/01/1998 |
Autoria: |
MONARES, A. |
Título: |
Un metodo de evaluacion economica de tecnologias de almacenamiento de semilla de papa. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
Lima: CIP, 1980. |
Páginas: |
15p. |
Série: |
(CIP. Documento de Entrenamiento, 1980-6). |
Idioma: |
Espanhol |
Palavras-Chave: |
Potato seed. |
Thesagro: |
Análise Econômica; Armazenamento; Batata Semente; Solanum Tuberosum. |
Thesaurus Nal: |
economic analysis; storage. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00582nam a2200205 a 4500 001 1758784 005 1998-01-28 008 1980 bl uuuu u0uu1 u #d 100 1 $aMONARES, A. 245 $aUn metodo de evaluacion economica de tecnologias de almacenamiento de semilla de papa. 260 $aLima: CIP$c1980 300 $a15p. 490 $a(CIP. Documento de Entrenamiento, 1980-6). 650 $aeconomic analysis 650 $astorage 650 $aAnálise Econômica 650 $aArmazenamento 650 $aBatata Semente 650 $aSolanum Tuberosum 653 $aPotato seed
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Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
15/10/2010 |
Data da última atualização: |
30/04/2018 |
Autoria: |
COSTA, A. T.; NUNES, W. M. de C.; ZANUTTO, C. A.; MÜLLER, G. W. |
Afiliação: |
Alessandra Tenório Costa, Universidade Estadual de Maringá; William Mário de Carvalho Nunes, Universidade Estadual de Maringá; Carlos Alexandre Zanutto, Universidade Estadual de Maringá; Gerd Walter Müller, Universidade Estadual de Maringá. |
Título: |
Stability of Citrus tristeza virus protective isolates in field conditions. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 693-700, jul. 2010 |
Idioma: |
Inglês |
Notas: |
Título em português: Estabilidade de isolados protetores contra Citrus tristeza virus em condições de campo. |
Conteúdo: |
The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products electrophoretic profiles were analyzed using the Jaccard coefficient and the UPGMA method. The majority of the clones had weak to moderate stem pitting symptoms and its CTV isolates showed alterations in the SSCP profiles. However, the stability of the protective complex has been maintained, except for isolates from two analised clones. Low genetic variability was observed within the isolates during the studied years. |
Palavras-Chave: |
Cross-protection; Polimorfismo conformacional da fita simples; Pré?imunização; Preimmunization; Proteção cruzada; Single strand conformational polymorphism; Sintoma de canelura. |
Thesagro: |
Citrus Sinensis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/105838/1/Stability.pdf
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Marc: |
LEADER 02116naa a2200265 a 4500 001 1864301 005 2018-04-30 008 2010 bl uuuu u00u1 u #d 100 1 $aCOSTA, A. T. 245 $aStability of Citrus tristeza virus protective isolates in field conditions. 260 $c2010 500 $aTítulo em português: Estabilidade de isolados protetores contra Citrus tristeza virus em condições de campo. 520 $aThe objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products electrophoretic profiles were analyzed using the Jaccard coefficient and the UPGMA method. The majority of the clones had weak to moderate stem pitting symptoms and its CTV isolates showed alterations in the SSCP profiles. However, the stability of the protective complex has been maintained, except for isolates from two analised clones. Low genetic variability was observed within the isolates during the studied years. 650 $aCitrus Sinensis 653 $aCross-protection 653 $aPolimorfismo conformacional da fita simples 653 $aPré?imunização 653 $aPreimmunization 653 $aProteção cruzada 653 $aSingle strand conformational polymorphism 653 $aSintoma de canelura 700 1 $aNUNES, W. M. de C. 700 1 $aZANUTTO, C. A. 700 1 $aMÜLLER, G. W. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 45, n. 7, p. 693-700, jul. 2010
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