| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Trigo. |
|
Data corrente: |
10/12/2010 |
|
Data da última atualização: |
26/08/2013 |
|
Autoria: |
EDEL, V.; STEINBERG, C.; AVELANGE, I.; LAGUERRE, G.; ALABOUVETTE, C. |
|
Título: |
Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. |
|
Ano de publicação: |
1995 |
|
Fonte/Imprenta: |
Phytopathology, St. Paul, v. 85, n. 5, p. 579-585, 1995. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Sixty strains of Fusarium oxysporum were characterized using three DNA-based methods: restriction fragment length polymorphism (RFLP) analysis of total DNA after hybridization with a random DNA probe polymerase chain reaction (PCR)-based fingerprinting with primers matching enterobacterial repetitive intergenic consensus (ERIC) sequences and repetitive extragenic palindromic (REP) elements, and restriction fragment analysis of PCR-amplified ribosomal intergenic spacers (IGS). All methods yielded intraspecific polymorphisms, but different levels of discrimination were obtained. Good correlation was found between the groupings obtained by the three methods. RFLP analysis of total DNA was the most sensitive method, enabling the detection of 40 variants within the sample of 60 strains. However, this technique is more time-consuming than the PCR-based methods. By ERIC- and REP-PCR fingerprinting and PCR/RFLP analysis of the IGS, the 60 strains were categorized into 27 and 11 genotypes, respectively. Though less discriminant, the PCR/RFLP method allowed estimation of the genetic relationships between the strains. Discrimination of closely related strains within IGS genotypes could be achieved by ERIC- or REP-PCR fingerprinting, which is the most efficient procedure in terms of simplicity and rapidity. Therefore, the two PCR-based procedures described in this paper appear to be rapid tools for the genetic characterization of large populations of F. oxysporum. |
|
Palavras-Chave: |
Repetitive DNA. |
|
Thesaurus Nal: |
DNA fingerprinting. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02030naa a2200193 a 4500
001 1869423
005 2013-08-26
008 1995 bl --- 0-- u #d
100 1 $aEDEL, V.
245 $aComparison of three molecular methods for the characterization of Fusarium oxysporum strains.
260 $c1995
520 $aSixty strains of Fusarium oxysporum were characterized using three DNA-based methods: restriction fragment length polymorphism (RFLP) analysis of total DNA after hybridization with a random DNA probe polymerase chain reaction (PCR)-based fingerprinting with primers matching enterobacterial repetitive intergenic consensus (ERIC) sequences and repetitive extragenic palindromic (REP) elements, and restriction fragment analysis of PCR-amplified ribosomal intergenic spacers (IGS). All methods yielded intraspecific polymorphisms, but different levels of discrimination were obtained. Good correlation was found between the groupings obtained by the three methods. RFLP analysis of total DNA was the most sensitive method, enabling the detection of 40 variants within the sample of 60 strains. However, this technique is more time-consuming than the PCR-based methods. By ERIC- and REP-PCR fingerprinting and PCR/RFLP analysis of the IGS, the 60 strains were categorized into 27 and 11 genotypes, respectively. Though less discriminant, the PCR/RFLP method allowed estimation of the genetic relationships between the strains. Discrimination of closely related strains within IGS genotypes could be achieved by ERIC- or REP-PCR fingerprinting, which is the most efficient procedure in terms of simplicity and rapidity. Therefore, the two PCR-based procedures described in this paper appear to be rapid tools for the genetic characterization of large populations of F. oxysporum.
650 $aDNA fingerprinting
653 $aRepetitive DNA
700 1 $aSTEINBERG, C.
700 1 $aAVELANGE, I.
700 1 $aLAGUERRE, G.
700 1 $aALABOUVETTE, C.
773 $tPhytopathology, St. Paul$gv. 85, n. 5, p. 579-585, 1995.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Trigo (CNPT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | GARDI, C.; ANGELINI, M.; BARCELÓ, S.; COMERMA, J.; CRUZ GAISTARDO, C.; ENCINA ROJAS, A.; JONES, A.; KRASILNIKOV, P.; MENDONÇA-SANTOS, M. L.; MONTANARELLA, L.; MUÑIZ UGARTE, O.; SCHAD, P.; VARA RODRÍGUEZ, M. I.; VARGAS, R.; RAVINA DA SILVA, M. (ed.). Atlas de solos da América Latina e do Caribe. Luxemburgo: Comissão Europeia, Serviço das Publicações da União Europeia, 2015. 176 p. il. color.| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Cocais; Embrapa Solos. |
|    |
| 2. | | GARDI, C.; ANGELINI, M.; BARCELÓ, S.; COMERMA, J.; CRUZ GAISTARDO, C.; ENCINA ROJAS, A.; JONES, A.; KRASILNIKOV, P.; MENDONÇA SANTOS BREFIN, M. L.; MONTANARELLA, L.; MUÑIZ UGARTE, O.; SCHAD, P.; VARA RODRÍGUEZ, M. I.; VARGAS, R. (ed.). Atlas de suelos de America Latina y el Caribe. Luxembourg: Comisión Europea, Oficina de Publicaciones de la Unión Europea, 2014. 176 p. il. color.| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Solos. |
| |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
