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Registros recuperados : 16 | |
4. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RODRIGUES, C. B.; BEZERRA, C. C. de O. N.; OLIVEIRA FILHO, J. G. de; FERREIRA, M. D. Atividade óleos essenciais como antimicrobianos naturais em pós colheita de frutos. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2021. Editores técnicos: Alexandre Berndt, Ana Rita de Araujo Nogueira, Lea Chapaval Andri, Marcelo Mattos Cavallari, Manuel Antonio Chagas Jacinto. 13 Biblioteca(s): Embrapa Instrumentação. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; FERREIRA, L. L.; SARRIA, A. L. F.; PICKETT, J. A.; MASCARIN, G. M.; PÉREZ DE LEÓN, A. A.; BORGES, L. M. F. Brown dog tick, Rhipicephalus sanguineus sensu lato, infestation of susceptible dog hosts is reduced by slow release of semiochemicals from a less susceptible host. Ticks and Tick-borne Diseases, v. 8, n. 1, p. 139-145, Jan. 2017. Biblioteca(s): Embrapa Arroz e Feijão. |
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9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO. J. G. de; BERTOLO, M. R. V.; RODRIGUES, M. A. V.; SILVA, G. C.; MENDONÇA, G. M. N. de; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Recent advances in the development of smart, active, and bioactive biodegradable biopolymer-based films containing betalains. Food Chemistry, v. 390, 133149, 2022. 13 p. Biblioteca(s): Embrapa Instrumentação. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; ALBIERO, B. R.; CIPRIANO, L.; BEZERRA, C. C. de O. N.; OLDONI, F. C. A.; EGEA, M. B.; AZEREDO, H. M. C. de; FERREIRA, M. D. Arrowroot starch-based films incorporated with a carnauba wax nanoemulsion, cellulose nanocrystals, and essential oils: a new functional material for food packaging applications. Cellulose, v. 28, 2021. 6499-6511 Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Instrumentação. |
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11. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BERTOLO, M. R. V.; DIAS, L. D.; OLIVEIRA FILHO, J. G. de; ALVES, F.; MARANGON, C. A.; MARTINS, V. C. A.; FERREIRA, M. D.; BAGNATO, V. S.; PLEPIS, A. M. G.; BOGUSZ JUNIOR, S. Central composite design optimization of active and physical properties of food packaging films based on chitosan/gelatin/pomegranate peel extract. Food Packaging and Shelf Life, v. 34, a100986, 2022. 1 - 11 Biblioteca(s): Embrapa Instrumentação. |
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12. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; BERTOLO, M. R. V.; FERNANDES, S. S.; LEMES, A. C.; SILVA, G. da C.; BOGUSZ JUNIOR, S.; AZEREDO, H. M. C. de; MATTOSO, L. H. C.; EGEA, M. B. Intelligent and active biodegradable biopolymeric films containing carotenoids. Food Chemistry, v. 434, 137454, 2024. Biblioteca(s): Embrapa Instrumentação. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; ALBIERO, B. R.; CALISTO, I. H.; BERTOLO, M. R. V.; OLDONI, F. C. A.; EGEA, M. B.; BOGUSZ JUNIOR, S.; AZEREDO, H. M. C. de; FERREIRA, M. D. Bio-nanocomposite edible coatings based on arrowroot starch/cellulose nanocrystals/carnauba wax nanoemulsion containing essential oils to preserve quality and improve shelf life of strawberry. International Journal of Biological Macromolecules, v. 219, 2022. 812 - 823 Biblioteca(s): Embrapa Instrumentação. |
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14. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; BERTOLO, M. R. V.; BRITO, S. C.; MALAFATTI, J. O. D.; BERTAZZO, G. B.; COLACIQUE, M. N.; PARIS, E. C.; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Recent advances in the application of nanotechnology to reduce fruit and vegetable losses during post-harvest. Brazilian Journal of Physics, v. 52, 126, 2022. 11 p. Biblioteca(s): Embrapa Instrumentação. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; DUARTE, L. G. R.; SILVA, Y. B. B.; MILAN, E. P.; SANTOS, H. V.; MOURA, T. C.; BANDINI, V. P.; VITOLANO, L. E. S.; NOBRE, J. J. C.; MOREIRA, C. T.; MITSUYUKI, M. C.; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Novel Approach for Improving Papaya Fruit Storage with Carnauba Wax Nanoemulsion in Combination with Syzigium aromaticum and Mentha spicata Essential Oils. Coatings, v. 13, n. 847, 2023. 16 p. Biblioteca(s): Embrapa Instrumentação. |
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16. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA FILHO, J. G. de; FERREIRA, L. L.; SILVA, F. de O.; MENEZES, C. M. F.; MUNIZ, E. R.; PAULA, L. G. F. de; SARRIA, A. L. F.; PICKETT, J. A.; BIRKETT, M. A.; MASCARIN, G. M.; BORGES, L. M. F. Persistence and efficacy of a new formulation based on dog allomonal repellents against Rhipicephalus sanguineus sensu lato tick. Revista Brasileira de Parasitologia Veterinária, v. 27, n. 3, p. 313-318, 2018. Biblioteca(s): Embrapa Meio Ambiente. |
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Registros recuperados : 16 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
30/08/2007 |
Data da última atualização: |
13/02/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
FARIA, R. O. de; MOURE, V. R.; BALTMANT, W.; AMAZONAS, M. A. L. de; KRIEGER, W.; MITCHELL, D. A. |
Afiliação: |
Rodrigo Otávio de Faria, UFPR; Vivian Rotuno Moure, UFPR; Wellington Balmant, UFPR; Maria Angela Lopes de Almeida Amazonas, Embrapa Florestas; Nadia Krieger, UFPR; David Alexander Mitchell, UFPR. |
Título: |
The tyrosinase produced by Lentinula boryana (Berk. & Mont.) pegler suffers substrate inhibition by L-DOPA. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Food Technology and Biotechnology, v. 45, n. 3, p. 334-340, 2007. |
Idioma: |
Inglês |
Conteúdo: |
We undertook a preliminary characterization of the tyrosinase produced by a strain of Lentinula boryana from Brazil, with a view to evaluate its potential for biotechnological applications. The enzyme was similar to other fungal tyrosinases in many respects. When the crude extract was characterized, the tyrosinase activity was optimal at pH=6 and was not particularly thermostable, with half-lives of about 10 min and 1 min at 50 and 60 °C, respectively. We purified the enzyme with ammonium sulfate precipitation followed by ion exchange chromatography on a DEAE Sepharose column, obtaining a yield of 33 % and a 5.3-fold enrichment. The purified preparation gave three bands on SDS-PAGE, with molecular masses of 20, 27 and 47 kDa. This preparation showed substrate inhibition kinetics with L-DOPA (3,4-dihydroxy-L-phenylalanine), with a KM of 1.9 mM and a KI of 72 mM. Under the same reaction conditions, a commercial mushroom tyrosinase followed Michaelis- Menten kinetics, with a KM of 0.51 mM. Although the present study did not identify properties that would make the tyrosinase of L. boryana more suitable in biotechnological applications than tyrosinases from other mushrooms, it has made a contribution by showing that the enzyme suffers substrate inhibition by L-DOPA, something that has not previously been reported for mushroom tyrosinases. |
Palavras-Chave: |
Inibição; Lentinula boryna; Substrato; Tirosinase. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02046naa a2200229 a 4500 001 1312078 005 2015-02-13 008 2007 bl uuuu u00u1 u #d 100 1 $aFARIA, R. O. de 245 $aThe tyrosinase produced by Lentinula boryana (Berk. & Mont.) pegler suffers substrate inhibition by L-DOPA.$h[electronic resource] 260 $c2007 520 $aWe undertook a preliminary characterization of the tyrosinase produced by a strain of Lentinula boryana from Brazil, with a view to evaluate its potential for biotechnological applications. The enzyme was similar to other fungal tyrosinases in many respects. When the crude extract was characterized, the tyrosinase activity was optimal at pH=6 and was not particularly thermostable, with half-lives of about 10 min and 1 min at 50 and 60 °C, respectively. We purified the enzyme with ammonium sulfate precipitation followed by ion exchange chromatography on a DEAE Sepharose column, obtaining a yield of 33 % and a 5.3-fold enrichment. The purified preparation gave three bands on SDS-PAGE, with molecular masses of 20, 27 and 47 kDa. This preparation showed substrate inhibition kinetics with L-DOPA (3,4-dihydroxy-L-phenylalanine), with a KM of 1.9 mM and a KI of 72 mM. Under the same reaction conditions, a commercial mushroom tyrosinase followed Michaelis- Menten kinetics, with a KM of 0.51 mM. Although the present study did not identify properties that would make the tyrosinase of L. boryana more suitable in biotechnological applications than tyrosinases from other mushrooms, it has made a contribution by showing that the enzyme suffers substrate inhibition by L-DOPA, something that has not previously been reported for mushroom tyrosinases. 653 $aInibição 653 $aLentinula boryna 653 $aSubstrato 653 $aTirosinase 700 1 $aMOURE, V. R. 700 1 $aBALTMANT, W. 700 1 $aAMAZONAS, M. A. L. de 700 1 $aKRIEGER, W. 700 1 $aMITCHELL, D. A. 773 $tFood Technology and Biotechnology$gv. 45, n. 3, p. 334-340, 2007.
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