Data/hora: 21/09/2024 08:12:25

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Data corrente: 	17/11/2020	
Data da última atualização: 	17/11/2020	
Autoria: 	MOURA, A. G. L.; RABELO, C. A. B. S.; OKINO, C. H.; MAINTINGUER, S. I.; SILVA, E. L.; VARESCHE, M. B. A.	
Afiliação: 	A. G .L. MOURA, USP; C. A. B. S. Rabelo, USP; CINTIA HIROMI OKINO, CPPSE; S. I. MAINTINGUER, UNESP; E. L. SILVA, UFSCAR; M. B. A. VARESCHE, USP.	
Título: 	Enhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: effects on hydA expression.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	International Journal of Hydrogen Energy, v. 45, n. 53, p. 28447-28431, oct. 2020.	
DOI: 	https://doi.org/10.1016/j.ijhydene.2020.07.161	
Idioma: 	Inglês	
Conteúdo: 	In this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H2) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H2 production (434 ± 13 mL) was achieved with 30 mg Fe0/L and 20 mg Ni0/L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one.	
Palavras-Chave: 	Dark fermentation; Iron nanoparticles; Nickel nanoparticles.	
Thesaurus Nal: 	Clostridium butyricum; Response surface methodology.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (25151-1) - UPC - AP - DD - PROCI-2020.00066 - MOU - 2020.00117 - DIS