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| Registros recuperados : 13 | |
| 3. |  | MOTA, F. F.; GOMES, E. A.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. Análise das comunidades bacterianas presentes nas rizosferas de variedades de milho sensíveis e tolerantes ao alumínio plantadas em solo de cerrado. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte, Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | MOTA, F. F.; GOMES, E. A.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. Efeito da calagem do solo de cerrado na estrutura de comunidades bacterianas e fungicas presentes na rizosfera de linhagens de milho (Zea mays L.) sensiveis e tolerantes ao alumínio. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24.; SIMPÓSIO BRASILEIRO DE MICROBACTÉRIAS, 12.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 2.; ENCONTRO DE ENSINO EM MICROBIOLOGIA, 4., 2007, Brasília, DF. Anais. [São Paulo]: SBM, 2007. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | MOTA, F. F. da; NOBREGA, F. F.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L. Genetic diversity of Paenibacillus polymyxa populations isolated from the rhizosphere of four cultivars of maize (Zea mays) planted in cerrado soil. Applied Soil Ecology, Amsterdam, v. 20, n. 2, p. 119-132, 2002.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | MOTA, F. F. da; GOMES, E.; MARRIEL, I.; PAIVA, E.; SELDIN, L. Molecular ecology based on 16S rDNA of bacterial communities from rhizospheres of sensitive and tolerant maize varieties under aluminum-stressing and non-stressing conditions thirty and ninety days after sowing. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito. Fertbio 2006: a busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | MOTA, F. F. da; GOMES, E. A.; PAIVA, E.; ROSADO, A. S.; SELDIN, L. Use of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene. Letters in Applied Microbiology, Oxford, v. 39, n. 1, p. 34-40, 2004.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | COELHO, M. R. R.; MOTA, F. F.; PAIVA, E.; MARRIEL, I. E.; ROSADO, A. S.; SELDIN, L.; CARNEIRO, N. P. Diversidade de Paenibacillus spp. na rizosfera de quatro cultivares de sorgo plantados em solo de cerrado sob condições contrastantes de nitrogênio. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | COELHO, M. R. R.; MOTA, F. F. da; CARNEIRO, N. P.; MARRIEL, I. E.; PAIVA, E.; ROSADO, A. S.; SELDIN, L. Diversity of Paenibacillus spp. in the rhizosphere of four sorghum (Sorghum bicolor) cultivars sown with two contrasting levels of nitrogen fertilizer assessed by rpoB-Based PCR-DGGE and sequencing analysis. Journal of Microbiology and Biotechnology, v. 17, n. 5, p. 753-760, 2007.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | COTTA, S. R; MOTA, F. F. da; TUPINAMBÁ, G.; ISHIDA, K.; ROZENTAL, S.; SILVA, D. O. e; SILVA, A. J. R. da; BIZZO, H. R.; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. World Journal of Microbiology and Biotechnology, v. 28, n. 3, p. 953-962, 2012.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 13 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
24/05/2012 |
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Data da última atualização: |
22/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
COTTA, S. R; MOTA, F. F. da; TUPINAMBÁ, G.; ISHIDA, K.; ROZENTAL, S.; SILVA, D. O. e; SILVA, A. J. R. da; BIZZO, H. R.; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. |
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Afiliação: |
SIMONE RAPOSO COTTA, UFRJ; FABIO FARIA DA MOTA, UFRJ; GLEISER TUPINAMBÁ, UFRJ; KELLY ISHIDA, UFRJ; SONIA ROZENTAL, UFRJ; DAVI OLIVEIRA E SILVA, UFRJ; ANTONIO JORGE RIBEIRO DA SILVA, UFRJ; HUMBERTO RIBEIRO BIZZO, CTAA; DANIELA SALES ALVIANO, UFRJ; CELUTA SALES ALVIANO, UFRJ; LUCY SELDIN, UFRJ. |
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Título: |
Antimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 28, n. 3, p. 953-962, 2012. |
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DOI: |
10.1007/s11274-011-0893-1 |
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Idioma: |
Inglês |
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Conteúdo: |
In a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes. MenosIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used again... Mostrar Tudo |
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Palavras-Chave: |
Dermatófitos patogênicos; Paenibacillus kribbensis; Substância antimicrobiana. |
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Thesaurus NAL: |
Trichophyton rubrum. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 1925383
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024 7 $a10.1007/s11274-011-0893-1$2DOI
100 1 $aCOTTA, S. R
245 $aAntimicrobial activity of Paenibacillus kribbensis POC 115 against the dermatophyte Trichophyton rubrum.
260 $c2012
520 $aIn a search for an antifungal substance with activity against the dermatophyte fungus Trichophyton rubrum, strain POC 115 was chosen among different Paenibacillus strains for its phenotypic and genetic characterization and for preliminary characterization of its antimicrobial substance. Strain POC 115 was identified as belonging to Paenibacillus kribbensis. Physico-chemical characterization of the antimicrobial substance showed that it was not stable during heat and organic solvents treatments, but its activity was preserved at a wide range of pH and after treatment with pronase E, trypsin and DNase I. The crude concentrated supernatant of POC 115 culture was partially purified and the fraction presenting antimicrobial activity was further analyzed by UPLC/Mass Spectrometry. Two peaks were observed at 2.02 (mass 1,207 D) and 2.71 (mass 1,014 D) min in the mass chromatogram. The antimicrobial substance produced by POC 115 was correlated to iturin family compounds based on a set of primers designed for the amplification of PKS operon in the POC 115 genome. As happens with the mode of action of the antibiotics of the iturin group, the AMS produced by POC 115 caused the disruption of cytoplasmic membrane of T. rubrum and the subsequent withdraw of the intracellular material. This is the first report on the production of antimicrobial substances in P. kribbensis, and it may be of great relevance as an alternative or supplementary substance to antifungal drugs currently used against dermatophytes.
650 $aTrichophyton rubrum
653 $aDermatófitos patogênicos
653 $aPaenibacillus kribbensis
653 $aSubstância antimicrobiana
700 1 $aMOTA, F. F. da
700 1 $aTUPINAMBÁ, G.
700 1 $aISHIDA, K.
700 1 $aROZENTAL, S.
700 1 $aSILVA, D. O. e
700 1 $aSILVA, A. J. R. da
700 1 $aBIZZO, H. R.
700 1 $aALVIANO, D. S.
700 1 $aALVIANO, C. S.
700 1 $aSELDIN, L.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 28, n. 3, p. 953-962, 2012.
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