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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/02/2009 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GODOY, L. P.; VASCONCELOS, A. T. R.; CHUEIRE, L. M. O.; SOUZA, R. C.; NICOLÁS, M. F.; BARCELLOS, F. G.; HUNGRIA, M. |
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Afiliação: |
Leandro Pereira Godoy, CNPSo/UEL; Ana Tereza Ribeiro Vasconcelos Laboratório Nacional de Computação Científica Petrópolis RJ; Ligia Maria Oliveira Chueire, CNPSo; Rangel Celso Souza Laboratório Nacional de Computação Científica Petrópolis RJ; Marisa Fabiana Nicolás Laboratório Nacional de Computação Científica Petrópolis RJ; Fernando Gomes Barcellos, CNPS; Mariangela Hungria, CNPSo. |
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Título: |
Genomic panorama of Bradyrhizobium japonicum CPAC 15, a commercial inoculant strain largely established in Brazilian soils and belonging to the same serogroup as USDA 123. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Soil Biology & Biochemistry, v. 40, n. 11, p. 2743-2753, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Of the many genomes of prokaryotes that have been sequenced, most are pathogenic organisms and very few of agriculturally beneficial bacteria. Soybean, the most important cash crop in Brazil, can provide its need for nitrogen through a symbiosis with exotic strains of bradyrhizobia. Bradyrhizobium japonicum strain CPAC 15 (equates to SEMIA 5079, the same serogroup as USDA 123), which is a highly competitive commercial strain applied to soybean crops since the early 1990s, is now established on several millions of hectares. As financial resources for sequencing genomes are still very limited in developing countries, a panoramic genomic view of CPAC 15 was generated. A total of 4328 shotgun reads resulted in 2,046,740 bp with a phred score_ 20; the assemblage resulted in 1106 phrap contigs scattered by 69 scaffolds and 966 isolated contigs, with an average of 2.5 reads per contig, covering approximately 13% of the genome. Annotation identified 1371 coding DNA sequences (CDSs), 53% with putative known functions, 23% encoding conserved hypothetical and 24% hypothetical genes, representing about 16% of the estimated putative genes. Several comparisons - on COG and KEGG databases, tRNAs, transposases, Gþ C content of CPAC 15 with the complete genome of B. japonicum strain USDA 110 indicated a successful coverage of the whole genome. However, the two strains were surprisingly different, as at least 35% of the CDS of CPAC 15 shows higher similarity to microorganisms other than strain USDA 110. Several new putative genes and others with low similarity to USDA 110, were identified. These were related to nodulation, interaction with the host plant and adaptation, e.g., nodB, nodW, ndvA, effector nopP, genes of secretion systems, transporters and environmentally related genes. MenosOf the many genomes of prokaryotes that have been sequenced, most are pathogenic organisms and very few of agriculturally beneficial bacteria. Soybean, the most important cash crop in Brazil, can provide its need for nitrogen through a symbiosis with exotic strains of bradyrhizobia. Bradyrhizobium japonicum strain CPAC 15 (equates to SEMIA 5079, the same serogroup as USDA 123), which is a highly competitive commercial strain applied to soybean crops since the early 1990s, is now established on several millions of hectares. As financial resources for sequencing genomes are still very limited in developing countries, a panoramic genomic view of CPAC 15 was generated. A total of 4328 shotgun reads resulted in 2,046,740 bp with a phred score_ 20; the assemblage resulted in 1106 phrap contigs scattered by 69 scaffolds and 966 isolated contigs, with an average of 2.5 reads per contig, covering approximately 13% of the genome. Annotation identified 1371 coding DNA sequences (CDSs), 53% with putative known functions, 23% encoding conserved hypothetical and 24% hypothetical genes, representing about 16% of the estimated putative genes. Several comparisons - on COG and KEGG databases, tRNAs, transposases, Gþ C content of CPAC 15 with the complete genome of B. japonicum strain USDA 110 indicated a successful coverage of the whole genome. However, the two strains were surprisingly different, as at least 35% of the CDS of CPAC 15 shows higher similarity to microorganisms other than strai... Mostrar Tudo |
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Thesagro: |
Solo. |
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Categoria do assunto: |
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Marc: |
LEADER 02467naa a2200205 a 4500
001 1469733
005 2017-08-03
008 2008 bl --- 0-- u #d
100 1 $aGODOY, L. P.
245 $aGenomic panorama of Bradyrhizobium japonicum CPAC 15, a commercial inoculant strain largely established in Brazilian soils and belonging to the same serogroup as USDA 123.
260 $c2008
520 $aOf the many genomes of prokaryotes that have been sequenced, most are pathogenic organisms and very few of agriculturally beneficial bacteria. Soybean, the most important cash crop in Brazil, can provide its need for nitrogen through a symbiosis with exotic strains of bradyrhizobia. Bradyrhizobium japonicum strain CPAC 15 (equates to SEMIA 5079, the same serogroup as USDA 123), which is a highly competitive commercial strain applied to soybean crops since the early 1990s, is now established on several millions of hectares. As financial resources for sequencing genomes are still very limited in developing countries, a panoramic genomic view of CPAC 15 was generated. A total of 4328 shotgun reads resulted in 2,046,740 bp with a phred score_ 20; the assemblage resulted in 1106 phrap contigs scattered by 69 scaffolds and 966 isolated contigs, with an average of 2.5 reads per contig, covering approximately 13% of the genome. Annotation identified 1371 coding DNA sequences (CDSs), 53% with putative known functions, 23% encoding conserved hypothetical and 24% hypothetical genes, representing about 16% of the estimated putative genes. Several comparisons - on COG and KEGG databases, tRNAs, transposases, Gþ C content of CPAC 15 with the complete genome of B. japonicum strain USDA 110 indicated a successful coverage of the whole genome. However, the two strains were surprisingly different, as at least 35% of the CDS of CPAC 15 shows higher similarity to microorganisms other than strain USDA 110. Several new putative genes and others with low similarity to USDA 110, were identified. These were related to nodulation, interaction with the host plant and adaptation, e.g., nodB, nodW, ndvA, effector nopP, genes of secretion systems, transporters and environmentally related genes.
650 $aSolo
700 1 $aVASCONCELOS, A. T. R.
700 1 $aCHUEIRE, L. M. O.
700 1 $aSOUZA, R. C.
700 1 $aNICOLÁS, M. F.
700 1 $aBARCELLOS, F. G.
700 1 $aHUNGRIA, M.
773 $tSoil Biology & Biochemistry$gv. 40, n. 11, p. 2743-2753, 2008.
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Embrapa Soja (CNPSO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
01/02/2012 |
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Data da última atualização: |
01/02/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
PEREIRA, e. DOS s.; JANK, L.; MORI, I. K.; MARCOS, M. F. |
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Afiliação: |
ÉDSON DOS SANTOS PEREIRA, UCDB; LIANA JANK, CNPGC; INARA KEIKO MORI, UCDB; MIRIAM FERREIRA MARCOS, UCDB. |
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Título: |
Produção de sementes de híbridos de Panicum maximum. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forage for climate change adaptation and mitigation - eco-efficient animal produtction: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. |
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Páginas: |
78-81 |
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Descrição Física: |
1 CD-ROM |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar a densidade de florescimento de híbridos e selecionar as famílias maiores produtoras de sementes. |
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Palavras-Chave: |
Embrapa Gado de Corte; Sementes. |
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Thesagro: |
Panicum Maximum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00859naa a2200205 a 4500
001 1914085
005 2012-02-01
008 2011 bl uuuu u00u1 u #d
100 1 $aPEREIRA, e. DOS s.
245 $aProdução de sementes de híbridos de Panicum maximum.
260 $c2011
300 $a78-81$c1 CD-ROM
520 $aO objetivo deste trabalho foi avaliar a densidade de florescimento de híbridos e selecionar as famílias maiores produtoras de sementes.
650 $aPanicum Maximum
653 $aEmbrapa Gado de Corte
653 $aSementes
700 1 $aJANK, L.
700 1 $aMORI, I. K.
700 1 $aMARCOS, M. F.
773 $tIn: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forage for climate change adaptation and mitigation - eco-efficient animal produtction: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011.
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