| |
|
|
| Registros recuperados : 6 | |
| 3. |  | MORAES, A. da C. L.; VIGNA, B. B. Z.; OLIVEIRA, F. A. de; SILVA, C. B. C.; VALLE, C. B. do; SOUZA, A. P. de. Development of single nucleotide polymorphism (SNP) markers for genetic map saturation of Urochloa humidicola. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz do Iguaçu, PR. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 46.| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 4. | | MARTINS, F. B.; AONO, A. H.; MORAES, A. da C. L.; FERREIRA, R. C. U.; VILELA, M. de M.; PESSOA FILHO, M. A. C. de P.; RODRIGUES-MOTTA, M.; SIMEÃO, R. M.; SOUZA, A. P. de. Genome-wide family prediction unveils molecular mechanisms underlying the regulation of agronomic traits in Urochloa ruziziensis. Frontiers in Plant Science, v. 14, 2023. Na publicação: Marco Pessoa-Filho.| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Corte. |
| |
| 5. | | MORAES, A. da C. L.; MOLLINARI, M.; FERREIRA, R. C. U.; AONO, A.; LARA, L. A. de C.; PESSOA FILHO, M. A. C. de P.; BARRIOS, S. C. L.; GARCIA, A. A. F.; VALLE, C. B. do; SOUZA, A. P. de; VIGNA, B. B. Z. Advances in genomic characterization of Urochloa humidicola: exploring polyploid inheritance and apomixis. Theoretical and Applied Genetics, v. 136, n. 11, 2023. 17 p.| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
|  |
| 6. | | AONO, A. H.; FERREIRA, R. C. U.; MORAES, A. da C. L.; LARA, L. A. de C.; PIMENTA, R. J. G.; COSTA, E. A.; PINTO, L. R.; LANDELL, M. G. de A.; SANTOS, M. F.; JANK, L.; BARRIOS, S. C. L.; VALLE, C. B.; CHIARI, L.; GARCIA, A. A. F.; KUROSHU, R. M.; LORENA, A. C.; GORJANC, G.; SOUZA, A. P. de. A joint learning approach for genomic prediction in polyploid grasses. Scientific Reports, 12, article 12499, 2022. 17 p.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| Registros recuperados : 6 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
|
Data corrente: |
16/03/2020 |
|
Data da última atualização: |
20/04/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
OKINO, C. H.; RECH, D. V.; JAENISCH, F. R. F.; TREVISOL, I. M.; REBELATTO, R.; COLDEBELLA, A.; MORES, M. A. Z.; GIGLIOTI, R.; VAZ, C. S. L. |
|
Afiliação: |
CINTIA HIROMI OKINO, CPPSE; DAIANE VOSS RECH, CNPSA; FATIMA REGINA FERREIRA JAENISCH, CNPSA; IARA MARIA TREVISOL, CNPSA; RAQUEL REBELATTO, CNPSA; ARLEI COLDEBELLA, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; RODRIGO GIGLIOTI, UNESP; CLARISSA SILVEIRA LUIZ VAZ, CNPSA. |
|
Título: |
Detecting infectious bursal disease using a VP1 gene-based RT-qPCR assay compared to standard methods of virus isolation, ELISA, and histopathology. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Current Microbiology, 2020. |
|
Páginas: |
8 p. |
|
ISSN: |
1432-0991 |
|
DOI: |
https://doi.org/10.1007/s00284-020-01906-7 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Infectious bursal disease (IBD) is an immunosuppressive viral disease of chickens, associated with severe economic losses and major threats to poultry production worldwide. Disease prevention programs rely on unequivocal identification of the pathogen, as well as vaccination programs. This study developed a sensitive, one-step, real-time, quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay using a hydrolysis probe system for infectious bursal disease virus (IBDV, VP1 gene) detection and quantification, which was compared to other routinely used diagnostic methods. The assay successfully detected IBD reference viruses and field isolates. The absence of cross-reactivity was detected with negative samples or with other avian viruses in the analytical specificity test. The detection limit of this assay was 70 RNA copies. RT-qPCR was more sensitive in the detection of serially diluted IBDV isolates compared to virus isolation. For clinical samples, the sensitivity and specificity values of RT-qPCR compared to enzyme-linked immunosorbent assay (ELISA) were 97.5% and 100%, respectively, and compared to histopathology, these values were 100% and 93.94%, respectively. RT-qPCR can provide a simple and reliable assay for IBDV surveillance programs and for evaluation of control strategies. |
|
Palavras-Chave: |
Doença infecciosa da bolsa (DII); Doença viral imunossupressora; Immunosuppressive viral disease of chickens; RT-qPCR. |
|
Thesagro: |
Avicultura; Elisa; Histopatologia; Produção Animal; Sanidade Animal; Virologia. |
|
Thesaurus NAL: |
Chickens; Infectious bursal disease. |
|
Categoria do assunto: |
--
H Saúde e Patologia |
|
Marc: |
LEADER 02532naa a2200397 a 4500
001 2121266
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
022 $a1432-0991
024 7 $ahttps://doi.org/10.1007/s00284-020-01906-7$2DOI
100 1 $aOKINO, C. H.
245 $aDetecting infectious bursal disease using a VP1 gene-based RT-qPCR assay compared to standard methods of virus isolation, ELISA, and histopathology.$h[electronic resource]
260 $c2020
300 $a8 p.
520 $aAbstract: Infectious bursal disease (IBD) is an immunosuppressive viral disease of chickens, associated with severe economic losses and major threats to poultry production worldwide. Disease prevention programs rely on unequivocal identification of the pathogen, as well as vaccination programs. This study developed a sensitive, one-step, real-time, quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay using a hydrolysis probe system for infectious bursal disease virus (IBDV, VP1 gene) detection and quantification, which was compared to other routinely used diagnostic methods. The assay successfully detected IBD reference viruses and field isolates. The absence of cross-reactivity was detected with negative samples or with other avian viruses in the analytical specificity test. The detection limit of this assay was 70 RNA copies. RT-qPCR was more sensitive in the detection of serially diluted IBDV isolates compared to virus isolation. For clinical samples, the sensitivity and specificity values of RT-qPCR compared to enzyme-linked immunosorbent assay (ELISA) were 97.5% and 100%, respectively, and compared to histopathology, these values were 100% and 93.94%, respectively. RT-qPCR can provide a simple and reliable assay for IBDV surveillance programs and for evaluation of control strategies.
650 $aChickens
650 $aInfectious bursal disease
650 $aAvicultura
650 $aElisa
650 $aHistopatologia
650 $aProdução Animal
650 $aSanidade Animal
650 $aVirologia
653 $aDoença infecciosa da bolsa (DII)
653 $aDoença viral imunossupressora
653 $aImmunosuppressive viral disease of chickens
653 $aRT-qPCR
700 1 $aRECH, D. V.
700 1 $aJAENISCH, F. R. F.
700 1 $aTREVISOL, I. M.
700 1 $aREBELATTO, R.
700 1 $aCOLDEBELLA, A.
700 1 $aMORES, M. A. Z.
700 1 $aGIGLIOTI, R.
700 1 $aVAZ, C. S. L.
773 $tCurrent Microbiology, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
