Data/hora: 09/06/2024 17:51:09

Biblioteca(s): | Embrapa Amazônia Ocidental.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | Internacional - A
Data corrente: | 19/09/2006
Data da última atualização: | 06/01/2015
Autoria: | FUKUMASU, H.; AVANZO, J. L.; HEIDOR, R.; SILVA, T. C.; ATROCH, A.; MORENO, F. S.; DAGLI, M. L. Z.
Afiliação: | H. Fukumasu, USP; J.L. Avanzo, USP; R. Heidor, USP; T.C. Silva, USP; André Atroch, CPAA; F.S. Moreno, USP; M.L.Z. Dagli, USP.
Título: | Protective effects of guarana (Paullinia cupana Mart. var. Sorbilis) against DEN-induced DNA damage on mouse liver.
Ano de publicação: | 2006
Fonte/Imprenta: | Food and Chemical Toxicology, v. 44, n. 6 , p. 862-867, jun. 2006.
Idioma: | Inglês
Conteúdo: | Guarana (Paullinia cupana Mart. var. Sorbilis) is a plant originally from Brazil, which is rich in tannins. Some tannins are known to present protective effects against DNA damage. This study was performed to investigate the anti-genotoxic/cytotoxic properties of guarana in hepatocytes of mice injected with N-nitrosodiethylamine (DEN). The protective effect of guarana was evaluated both by comet assay and DNA smear fragmentation technique in two month-old female BALB/c mice. These were treated previously with 2.0 mg/g bw of guarana for 16 days and then injected with DEN (160 µg/g body weight) to induce DNA damage. The DEN-only treated group presented higher comet image length than the guarana plus DEN and untreated groups (116.06 ± 5.0 µm, 104.09 ± 3.3 µm and 93.28 ± 14.4 µm, respectively; p < 0.01). Guarana treatment presented a 52.54% reduction in comet image length when animals were exposed to DEN (p < 0.05). DNA samples from the guarana plus DEN group clearly showed less EtBr fluorescence intensity when compared to the DEN-only group, reinforcing the comet assay data. These results show, for the first time, that guarana has a protective effect against DEN-induced DNA damage in mouse liver.
Thesagro: | DNA; Guaraná; Paullinia Cupana.
Categoria do assunto: | --
Registro original: | Embrapa Amazônia Ocidental (CPAA)
Exemplares: | CPAA - (15484-1) - UPC - AP - PP - S8456 -  - S8456 - DIS