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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
18/03/2022 |
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Data da última atualização: |
18/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, J. C.; SOARES, A. C.; ANGELIM, M. K. S. C.; PROENÇA-MODENA, J. L.; VIEIRA, P. M. M.; MATTOSO, L. H. C.; OLIVEIRA JR, O. N. |
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Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Diagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Talanta, v. 239, 123076, 2022. |
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Páginas: |
1 - 7 |
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ISSN: |
0039-9140 |
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DOI: |
https://doi.org/10.1016/j.talanta.2021.123076 |
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Idioma: |
Inglês |
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Conteúdo: |
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. MenosMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be ap... Mostrar Tudo |
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Palavras-Chave: |
Impedance spectroscopy; Information visualization; SARS-CoV-2; Spike protein. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02452naa a2200277 a 4500
001 2141031
005 2022-03-18
008 2022 bl uuuu u00u1 u #d
022 $a0039-9140
024 7 $ahttps://doi.org/10.1016/j.talanta.2021.123076$2DOI
100 1 $aSOARES, J. C.
245 $aDiagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein.$h[electronic resource]
260 $c2022
300 $a1 - 7
520 $aMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources.
653 $aImpedance spectroscopy
653 $aInformation visualization
653 $aSARS-CoV-2
653 $aSpike protein
700 1 $aSOARES, A. C.
700 1 $aANGELIM, M. K. S. C.
700 1 $aPROENÇA-MODENA, J. L.
700 1 $aVIEIRA, P. M. M.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA JR, O. N.
773 $tTalanta$gv. 239, 123076, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
29/03/2022 |
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Data da última atualização: |
29/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DAWSON, M.; RIBEIRO, C.; MORELLI, M. R. |
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Afiliação: |
CAUE RIBEIRO DE OLIVEIRA, CNPDIA. |
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Título: |
MnCl2 doping increases phase stability of tin halide perovskites. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Materials Science in Semiconductor Processing, v. 132, 105908, 2021. |
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Páginas: |
1 - 11 |
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ISSN: |
1369-8001 |
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DOI: |
https://doi.org/10.1016/j.mssp.2021.105908 |
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Idioma: |
Inglês |
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Conteúdo: |
Tin halide perovskite (CH3NH3SnI3) suffers from instability due to the oxidation of tin(II). Doping tin(II) with cations can modify the properties of CH3NH3SnI3 and consequently, its stability. The gains and associated mechanisms differ according to cation. Here, MnCl2 is highlighted as a potential transition metal dopant. Using MnCl2, 2 and 10 mol% Mn doped CH3NH3SnI3 films were synthesized by the one-step method and characterized. Based on X-ray diffraction and micro-Raman measurements, the perovskite structure is formed irrespective of MnCl2 doping. The distribution of Mn was satisfactory as per Energy Dispersive X-ray mapping and chlorine from the precursor is not eliminated. Thus, must be considered when choosing MnCl2 as a dopant. The diffraction patterns of aged samples (24 h in air) indicate partial stabilization with Mn doping. From X-ray photoelectron spectroscopy (XPS) analysis, the stability of tin(II) in 10% Mn was slightly improved, tin vacancies were reduced and SnO2 formation was lowered despite Sn(IV) species being a little higher than the pristine sample. In general, the findings are promising for the development of stable tin perovskite solar cells. |
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Palavras-Chave: |
Manganese doping; Perovskite stability; Solar energy material; Tin halide perovskite. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01881naa a2200229 a 4500
001 2141566
005 2022-03-29
008 2022 bl uuuu u00u1 u #d
022 $a1369-8001
024 7 $ahttps://doi.org/10.1016/j.mssp.2021.105908$2DOI
100 1 $aDAWSON, M.
245 $aMnCl2 doping increases phase stability of tin halide perovskites.$h[electronic resource]
260 $c2022
300 $a1 - 11
520 $aTin halide perovskite (CH3NH3SnI3) suffers from instability due to the oxidation of tin(II). Doping tin(II) with cations can modify the properties of CH3NH3SnI3 and consequently, its stability. The gains and associated mechanisms differ according to cation. Here, MnCl2 is highlighted as a potential transition metal dopant. Using MnCl2, 2 and 10 mol% Mn doped CH3NH3SnI3 films were synthesized by the one-step method and characterized. Based on X-ray diffraction and micro-Raman measurements, the perovskite structure is formed irrespective of MnCl2 doping. The distribution of Mn was satisfactory as per Energy Dispersive X-ray mapping and chlorine from the precursor is not eliminated. Thus, must be considered when choosing MnCl2 as a dopant. The diffraction patterns of aged samples (24 h in air) indicate partial stabilization with Mn doping. From X-ray photoelectron spectroscopy (XPS) analysis, the stability of tin(II) in 10% Mn was slightly improved, tin vacancies were reduced and SnO2 formation was lowered despite Sn(IV) species being a little higher than the pristine sample. In general, the findings are promising for the development of stable tin perovskite solar cells.
653 $aManganese doping
653 $aPerovskite stability
653 $aSolar energy material
653 $aTin halide perovskite
700 1 $aRIBEIRO, C.
700 1 $aMORELLI, M. R.
773 $tMaterials Science in Semiconductor Processing$gv. 132, 105908, 2021.
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