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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/05/2006 |
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Data da última atualização: |
24/05/2006 |
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Autoria: |
JUNGHANS, T. G.; BARROS, E. G. de; MOREIRA, M. A. |
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Afiliação: |
EMBRAPA-CNPMF. |
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Título: |
Isolation of a partial cDNA of lipoxygenase from soybean nodule. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Magistra, Cruz das Almas, v. 16, n. 1, p. 47-53, jan./jun., 2004. |
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ISSN: |
0102-5333 |
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Idioma: |
Português |
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Conteúdo: |
Two specific primers were used to amplify possible lipoxygenase DNA fragments from root and nodule cDNAs of soybean plants. At least a major form of lipoxygenase expressed in the root was not expressed in the nodule. In order to infer putative functions of lipoxygenase present in the nodule, the PCR fragment from the nodule was cloned and sequenced resulting in a 356 bp sequence. The amino acid derived sequence of this cDNS clone, GmLOXN, when compared with others in the GenBank revealed to be a new form of a lipoxygenase gene. The homology of 69% of GmLOXN with vlxC, a lipoxygenase with possible role in temporary nitrogen storage, and the fact that this protein type accumulates in soybean in response to nitrogen fertilization, suggests the possibility of GmLOXN to be part of a gene involved in the temporary nitrogen storage in the nodules for such structures presenting high levels of nitrogen fixed. |
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Palavras-Chave: |
PCR; temporary nitrogen storage. |
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Thesagro: |
Glycine Max. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01449naa a2200193 a 4500
001 1653688
005 2006-05-24
008 2004 bl uuuu u00u1 u #d
022 $a0102-5333
100 1 $aJUNGHANS, T. G.
245 $aIsolation of a partial cDNA of lipoxygenase from soybean nodule.
260 $c2004
520 $aTwo specific primers were used to amplify possible lipoxygenase DNA fragments from root and nodule cDNAs of soybean plants. At least a major form of lipoxygenase expressed in the root was not expressed in the nodule. In order to infer putative functions of lipoxygenase present in the nodule, the PCR fragment from the nodule was cloned and sequenced resulting in a 356 bp sequence. The amino acid derived sequence of this cDNS clone, GmLOXN, when compared with others in the GenBank revealed to be a new form of a lipoxygenase gene. The homology of 69% of GmLOXN with vlxC, a lipoxygenase with possible role in temporary nitrogen storage, and the fact that this protein type accumulates in soybean in response to nitrogen fertilization, suggests the possibility of GmLOXN to be part of a gene involved in the temporary nitrogen storage in the nodules for such structures presenting high levels of nitrogen fixed.
650 $aGlycine Max
653 $aPCR
653 $atemporary nitrogen storage
700 1 $aBARROS, E. G. de
700 1 $aMOREIRA, M. A.
773 $tMagistra, Cruz das Almas$gv. 16, n. 1, p. 47-53, jan./jun., 2004.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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