Data/hora: 22/09/2024 12:08:19

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	13/10/2021	
Data da última atualização: 	13/10/2021	
Autoria: 	PETRY, B.; MOREIRA, G. C. M.; COPOLA, A. G. L.; SOUZA, M. M. de; VEIGA, F. C. da; JORGE, E. C.; PEIXOTO, J. de O.; LEDUR, M. C.; KOLTES, J. E.; COUTINHO, L. L.	
Afiliação: 	BRUNA PETRY, Esalq; GABRIEL COSTA MONTEIRO MOREIRA, Esalq; ALINE GONÇALVES LIO COPOLA, UFMG; MARCELA MARIA DE SOUZA, Iowa State University; FERNANDA CRISTINA DA VEIGA, Esalq; ERIKA CRISTINA JORGE, UFMG; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; JAMES E. KOLTES, Iowa State University; LUIZ LEHMANN COUTINHO, Esalq.	
Título: 	SAP30 gene is a probable regulator of muscle hypertrophy in chickens.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Frontiers in Genetic, v. 12, n. 709937, 2021.	
DOI: 	10.3389/fgene.2021.709937	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.	
Palavras-Chave: 	C2C12; Crescimento de células musculares; Hipertrofia; Knockdown; Muscle cell growth; Qualidade da carne; SiRNA.	
Thesagro: 	Frango de Corte; Genética Animal; Genética Molecular; Melhoramento Genético Animal; Produção de Carne.	
Thesaurus Nal: 	Hypertrophy; Skeletal muscle.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (22221-1) - UPC - AP - DD -  -  -  - DIS