Data/hora: 22/09/2024 11:49:56

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	15/10/2015	
Data da última atualização: 	15/03/2016	
Autoria: 	GODOY, T. F.; MOREIRA, G. C. M.; BOSCHIERO, C.; GHEYAS, A. A.; GASPARIN, G.; PADUAN, M.; ANDRADE, S. C. S.; MONTENEGRO, H.; BURT, D. W.; LEDUR, M. C.; COUTINHO, L. L.	
Afiliação: 	THAYS FERNANDA GODOY, ESALQ; GABRIEL COSTA MONTEIRO MOREIRA, ESALQ; CLARISSA BOSCHIERO, ESALQ; ALMAS A. GHEYAS, University of Edinburgh; GUSTAVO GASPARIN, ESALQ; MARCELA PADUAN, ESALQ; SONIA CRISTINA DA SILVA ANDRADE, ESALQ; HORACIO MONTENEGRO, ESALQ; DAVID W. BURT, University of Edinburgh; MONICA CORREA LEDUR, CNPSA; LUIZ LEHMANN COUTINHO, ESALQ.	
Título: 	SNP and INDEL detection in a QTL region on chicken chromosome 2 associated with muscle deposition.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Animal Genetics, v. 6, n. 2, p. 158-163, 2015.	
DOI: 	10.1111/age.12271	
Idioma: 	Inglês	
Conteúdo: 	Genetic improvement is important for the poultry industry, contributing to increased efficiency of meat production and quality. Because breast muscle is the most valuable part of the chicken carcass, knowledge of polymorphisms influencing this trait can help breeding programs. Therefore, the complete genome of 18 chickens from two different experimental lines (broiler and layer) from EMBRAPA was sequenced, and SNPs and INDELs were detected in a QTL region for breast muscle deposition on chicken chromosome 2 between microsatellite markers MCW0185 and MCW0264 (105 849?112 649 kb). Initially, 94 674 unique SNPs and 10 448 unique INDELs were identified in the target region. After quality filtration, 77% of the SNPs (85 765) and 60% of the INDELs (7828) were retained. The studied region contains 66 genes, and functional annotation of the filtered variants identified 517 SNPs and three INDELs in exonic regions. Of these, 357 SNPs were classified as synonymous, 153 as nonsynonymous, three as stopgain, four INDELs as frameshift and three INDELs as nonframeshift. These exonic mutations were identified in 37 of the 66 genes from the target region, three of which are related to muscle development (DTNA, RB1CC1 and MOS). Fifteen non-tolerated SNPs were detected in several genes (MEP1B, PRKDC, NSMAF, TRAPPC8, SDR16C5, CHD7, ST18 and RB1CC1). These loss-of-function and exonic variants present in genes related to muscle development can be considered candidate variants for further studies in chickens. Further association studies should be performed with these candidate mutations as should validation in commercial populations to allow a better explanation of QTL effects.	
Palavras-Chave: 	Músculo de peito; SNPs.	
Thesagro: 	Frango; Melhoramento genético animal; Mutação.	
Thesaurus Nal: 	Animal genetics; Breast muscle; Broiler chickens; Genetic polymorphism; Quantitative trait loci; Single nucleotide polymorphism.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (20287-1) - UPC - AP - DD -  -  -  - DIS