Data/hora: 09/06/2024 20:47:55

Biblioteca(s): | Embrapa Soja.
Data corrente: | 07/04/2004
Data da última atualização: | 23/04/2012
Autoria: | WENDLAND, A.; SILVA, J. F. V.; BINNECK, E.; MARIN, S. R. R.; MORALES, A. M. R.; TRAVENSOLO, R. F.; ALVES, L. C.; LEMOS, E. G. M.; MENTEN, J. O. M.; NEPOMUCENO, A. L.
Título: | Genetic expression of soybeans: Meloidogyne javanica interaction, revealed by analyses of microarrays.
Ano de publicação: | 2004
Fonte/Imprenta: | In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
Páginas: | p. 320.
Série: | (Embrapa Soja. Documentos, 228).
Idioma: | Inglês
Notas: | Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
Conteúdo: | The root-knot nematode, Meloidogyne javanica is an economically important pathogen of soybeans. Effective management of the nematode is often dependent on the use of resistant genotypes. The genetic interactions are complex and poorly understood. The use of cDNA Microarray technology promises to accelerate rapidly our understand of infectious disease processes, by allowing the examination of thousands of genes and gene products, simultaneously. To explore the plant genes putatively involved in the events of nematode-plant parasitism, we used Affymetrix GeneChips to conduct expression profiling of the early stages of infection of soybean genotypes. The infection experiments were conducted with three treatments: mock infected, resistant soybean genotype JF 7056 infected and suscetible soybean genotype 266-S infected with second stage juveniles - J2 of M. javanica. RNA obtained from the roots of each treatment was used to construct cDNA libraries, used for late sequencing. About 1000 unique sequences cDNA were chosen to be plotted in Microrray slides. Target cDNA was labelled by reverse transcription and incorporated with aminoallyl modified dUTP. A total of six hibridizations were performed. Sequences analyzed identified genes related to nematode resistance (ex: gene Hs1pro -1), and other stresses.
Thesagro: | Nematóide.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (23773-1) - UMT - PL - -- - RF 633.34072 - W927a -  - DIS