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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
16/01/2014 |
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Data da última atualização: |
20/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TIZIOTO, P. C.; GASPARIN, G.; SOUZA, M. M.; MUDADU, M. de A.; COUTINHO, L. L.; MOURÃO, G. B.; THOLON, P.; MEIRELLES, S. L. C.; TULLIO, R. R.; ROSA, A. do N.; ALENCAR, M. M. de; MEDEIROS, S. R. de; SIQUEIRA, F.; FEIJO, G. L. D.; NASSU, R. T.; REGITANO, L. C. de A. |
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Afiliação: |
POLYANA C. TIZIOTO, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo; GUSTAVO GASPARIN, Departamento de Zootecnia, Universidade de São Paulo/ESALQ, Piracicaba, São Paulo; MARCELA M. SOUZA, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo; MAURICIO DE ALVARENGA MUDADU, CPPSE; LUIZ L. COUTINHO, Departamento de Zootecnia, Universidade de São Paulo/ESALQ, Piracicaba, São Paulo; GERSON B. MOURÃO, Departamento de Zootecnia, Universidade de São Paulo/ESALQ, Piracicaba, São Paulo; PATRICIA THOLON, CPPSE; SARAH L. C. MEIRELLES, Departamento de Zootecnia, Universidade Federal de Lavras, Lavras, Minas Gerais; RYMER RAMIZ TULLIO, CPPSE; ANTONIO DO NASCIMENTO ROSA, CNPGC; MAURICIO MELLO DE ALENCAR, CPPSE; SERGIO RAPOSO DE MEDEIROS, CNPGC; FABIANE SIQUEIRA, CNPGC; GELSON LUIS DIAS FEIJO, CNPGC; RENATA TIEKO NASSU, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Identification of KCNJ11 as a functional candidate gene for bovine meat tenderness. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Physiological Genomics, v. 45, n. 24, p. 1215-1221, October 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
The potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P<=0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P<=0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction. MenosThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P<=0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P<=0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which ... Mostrar Tudo |
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Palavras-Chave: |
Differential expression. |
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Thesagro: |
Carne; Marcador molecular. |
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Thesaurus Nal: |
Beef; Genetic markers; Shear stress. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172838/1/4-Identification-of-KCNJ11-as-a-functional-candidate-gene-for-bovine-meat-2013.pdf
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Marc: |
LEADER 02703naa a2200373 a 4500
001 1976393
005 2018-02-20
008 2013 bl uuuu u00u1 u #d
100 1 $aTIZIOTO, P. C.
245 $aIdentification of KCNJ11 as a functional candidate gene for bovine meat tenderness.$h[electronic resource]
260 $c2013
520 $aThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P<=0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P<=0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction.
650 $aBeef
650 $aGenetic markers
650 $aShear stress
650 $aCarne
650 $aMarcador molecular
653 $aDifferential expression
700 1 $aGASPARIN, G.
700 1 $aSOUZA, M. M.
700 1 $aMUDADU, M. de A.
700 1 $aCOUTINHO, L. L.
700 1 $aMOURÃO, G. B.
700 1 $aTHOLON, P.
700 1 $aMEIRELLES, S. L. C.
700 1 $aTULLIO, R. R.
700 1 $aROSA, A. do N.
700 1 $aALENCAR, M. M. de
700 1 $aMEDEIROS, S. R. de
700 1 $aSIQUEIRA, F.
700 1 $aFEIJO, G. L. D.
700 1 $aNASSU, R. T.
700 1 $aREGITANO, L. C. de A.
773 $tPhysiological Genomics$gv. 45, n. 24, p. 1215-1221, October 2013.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/03/2013 |
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Data da última atualização: |
06/03/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CÂNDIDO, M. J. D.; LOPES, M. N.; POMPEU, R. C. F. F.; SILVA, R. G. da; CARVALHO, T. C. F. de; MORAIS NETO, L. B. de; MARANHÃO, T. D.; SILVEIRA, W. M. |
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Afiliação: |
Magno José Duarte Cândido, Universidade Federal do Ceará (UFC) - Fortaleza, CE; Marcos Neves Lopes, UFC - Fortaleza, CE; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; Rodrigo Gregório da Silva, Instituto Federal de Educação, Ciência e Tecnologia (IFCE) - Campus Crateús - Crateús, CE; Thaíse Cristine Ferreira de Carvalho, UFC - Fortaleza, CE; Luiz Barreto de Morais Neto, UFC - Fortaleza, CE; Theyson Duarte Maranhão, UFC - Fortaleza, CE; Walisson Marques Silveira, UFC - Fortaleza, CE. |
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Título: |
Densidade e estrutura do resíduo pós-pastejo em capim-massai adubado com nitrogênio e pastejado por ovinos. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 22., 2012, Cuiabá. A importância da zootecnia para a segurança alimentar: [anais]. Cuiabá: Universidade Federal de Mato Grosso, 2012. 3 f. 1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Somando-se ao suprimento nutricional, em especial a adubação nitrogenada, é imperativo o monitoramento rigoroso da condição residual do pasto. Objetivou-se avaliar a densidade e a estrutura do resíduo pós-pastejo em capim-massai adubado com N (0; 400; 800 e 1200 kg de N?ha-1?ano-1) e pastejado por ovinos num delineamento inteiramente casualizado com medidas repetidas no tempo. A elevação das doses de N influenciou as variáveis: densidade total de forragem residual, de forragem verde residual, de colmo verde residual, a relação lâmina foliar/colmo e o índice de qualidade da biomassa residual. Os ciclos de pastejo não exerceram alterações sobre a relação material vivo/material morto residual e densidade de forragem verde residual, sendo as demais variáveis modificadas pelos ciclos. A adubação nitrogenada proporciona respostas positivas sobre a densidade e estrutura do resíduo pós-pastejo do capim-massai. After grazing canopy structure and density of massai grass fertilized with nitrogen and grazed by sheep. Abstract: In addition to the nutrient supply, especially nitrogen, it is imperative to monitor carefully the residual condition of grass. To evaluate after grazing canopy structure and density of massai grass fertilized with nitrogen (0, 400, 800 and 1200 kg N?ha-1?year-1) and grazed by sheep in a completely randomized design with repeated measurements over time, this research was conducted. The nitrogen levels influenced the variables: residual total herbage density, residual green herbage density, residual green stem density, leaf/culm ratio and residual biomass quality index. The grazing cycles did not exert changes on the residual alive/senescent relation and residual green herbage density, being the others variables modified by the cycles. The nitrogen provides positive responses on after grazing canopy structure and density of massai grass. MenosResumo: Somando-se ao suprimento nutricional, em especial a adubação nitrogenada, é imperativo o monitoramento rigoroso da condição residual do pasto. Objetivou-se avaliar a densidade e a estrutura do resíduo pós-pastejo em capim-massai adubado com N (0; 400; 800 e 1200 kg de N?ha-1?ano-1) e pastejado por ovinos num delineamento inteiramente casualizado com medidas repetidas no tempo. A elevação das doses de N influenciou as variáveis: densidade total de forragem residual, de forragem verde residual, de colmo verde residual, a relação lâmina foliar/colmo e o índice de qualidade da biomassa residual. Os ciclos de pastejo não exerceram alterações sobre a relação material vivo/material morto residual e densidade de forragem verde residual, sendo as demais variáveis modificadas pelos ciclos. A adubação nitrogenada proporciona respostas positivas sobre a densidade e estrutura do resíduo pós-pastejo do capim-massai. After grazing canopy structure and density of massai grass fertilized with nitrogen and grazed by sheep. Abstract: In addition to the nutrient supply, especially nitrogen, it is imperative to monitor carefully the residual condition of grass. To evaluate after grazing canopy structure and density of massai grass fertilized with nitrogen (0, 400, 800 and 1200 kg N?ha-1?year-1) and grazed by sheep in a completely randomized design with repeated measurements over time, this research was conducted. The nitrogen levels influenced the variables: residual total herbage densit... Mostrar Tudo |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/78182/1/aac-Densidade-e-estruturas.pdf
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Marc: |
LEADER 02663nam a2200193 a 4500
001 1952354
005 2013-03-06
008 2012 bl uuuu u00u1 u #d
100 1 $aCÂNDIDO, M. J. D.
245 $aDensidade e estrutura do resíduo pós-pastejo em capim-massai adubado com nitrogênio e pastejado por ovinos.
260 $aIn: CONGRESSO BRASILEIRO DE ZOOTECNIA, 22., 2012, Cuiabá. A importância da zootecnia para a segurança alimentar: [anais]. Cuiabá: Universidade Federal de Mato Grosso, 2012. 3 f. 1 CD-ROM.$c2012
520 $aResumo: Somando-se ao suprimento nutricional, em especial a adubação nitrogenada, é imperativo o monitoramento rigoroso da condição residual do pasto. Objetivou-se avaliar a densidade e a estrutura do resíduo pós-pastejo em capim-massai adubado com N (0; 400; 800 e 1200 kg de N?ha-1?ano-1) e pastejado por ovinos num delineamento inteiramente casualizado com medidas repetidas no tempo. A elevação das doses de N influenciou as variáveis: densidade total de forragem residual, de forragem verde residual, de colmo verde residual, a relação lâmina foliar/colmo e o índice de qualidade da biomassa residual. Os ciclos de pastejo não exerceram alterações sobre a relação material vivo/material morto residual e densidade de forragem verde residual, sendo as demais variáveis modificadas pelos ciclos. A adubação nitrogenada proporciona respostas positivas sobre a densidade e estrutura do resíduo pós-pastejo do capim-massai. After grazing canopy structure and density of massai grass fertilized with nitrogen and grazed by sheep. Abstract: In addition to the nutrient supply, especially nitrogen, it is imperative to monitor carefully the residual condition of grass. To evaluate after grazing canopy structure and density of massai grass fertilized with nitrogen (0, 400, 800 and 1200 kg N?ha-1?year-1) and grazed by sheep in a completely randomized design with repeated measurements over time, this research was conducted. The nitrogen levels influenced the variables: residual total herbage density, residual green herbage density, residual green stem density, leaf/culm ratio and residual biomass quality index. The grazing cycles did not exert changes on the residual alive/senescent relation and residual green herbage density, being the others variables modified by the cycles. The nitrogen provides positive responses on after grazing canopy structure and density of massai grass.
700 1 $aLOPES, M. N.
700 1 $aPOMPEU, R. C. F. F.
700 1 $aSILVA, R. G. da
700 1 $aCARVALHO, T. C. F. de
700 1 $aMORAIS NETO, L. B. de
700 1 $aMARANHÃO, T. D.
700 1 $aSILVEIRA, W. M
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