Data/hora: 10/06/2024 03:33:23

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 21/12/2023
Data da última atualização: | 21/12/2023
Autoria: | CAVALCANTE, K. N.; FEITOR, J. F.; MORAIS, S. T. B.; NASSU, R. T.; AHRNÉ, L. M.; CARDOSO, D. R.
Afiliação: | KEILA N. CAVALCANTE, Universidade de São Paulo; JESSICA F. FEITOR, Universidade de São Paulo; SINARA T. B. MORAIS, Universidade de São Paulo; RENATA TIEKO NASSU, CPPSE; LILIA M. AHRNÉ, University of Copenhagen; DANIEL R. CARDOSO, Universidade de São Paulo.
Título: | Impact of UV-C pretreatment on B-lactoglobulin hydrolysis by trypsin: production and bioavailability of bioactive peptides.
Ano de publicação: | 2023
Fonte/Imprenta: | International Dairy Journal, v. 142, july 2023, 105650.
Páginas: | 12 p.
DOI: | 10.1016/j.idairyj.2023.105650
Idioma: | Inglês
Conteúdo: | The effects of UV-C light irradiation and low-temperature long-time (LTLT) pasteurization on protein structural changes, degree of hydrolysis (DH) by trypsin, peptide profile of tryptic hydrolysates by MALDI-TOF/TOF-MS, and bioavailability of β-lactoglobulin were compared. Compared with native or LTLT pasteurised samples, the hydrolysis rate constant of β-lactoglobulin treated with UV-C increased significantly, implying that the protein backbone cleavage sites became more accessible, whereas thermal treatment produced aggregates that impede trypsin activity. Tryptic hydrolyses of UV-C light treated β-lactoglobulin yielded more peptides and a more diverse peptide mass profile. Six bioactive peptides were revealed in β-LG tryptic hydrolysates of UV-C-treated protein; transepithelial transport in Caco-2 cell monolayers showed intermediate in vivo transport and absorption for three (β-LG f87–91, β-LG f91–99, and β-LG f158–164). The moderate allergen peptide LSFNPTQLEEQCHI β-LG was absent after tryptic hydrolysis of UV-C-treated protein, indicating that UV-C photolysis may be a useful tool for allergenicity reduction.
Palavras-Chave: | Bioactive peptides; LTLT; Luz UV-C; MALDI TOF TOF-MS; Peptídeos bioativos; Redução de alergenicidade.
Thesagro: | Tripsina.
Categoria do assunto: | Q Alimentos e Nutrição Humana
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (26268-1) - UPC - AP - DD - PROCI-2023.00126 - CAV - 2023.00299 - DIS