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| Registros recuperados : 15 | |
| 5. |  | AMARAL JUNIOR, J. M. do; MORAIS, E. de; SILVA, B. A. da; NAHUM, B. de S.; BEZERRA, A. S.; MARTORANO, L. G.; SILVA, A. G. M. e. Comportamento ingestivo de fêmeas bubalinas suplementadas com torta de palmiste sob condições térmico-hídricas na Amazônia Oriental. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., 2015, Belo Horizonte. Zootecnia: otimizando recursos e potencialidades: anais. Brasília, DF: Sociedade Brasileira de Zootecnia, 2015.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | ROGERIO, M. C. P.; ARAÚJO, A. R.; POMPEU, R. C. F. F.; SILVA, A. G. M. e; MORAIS, E. de; MEMÓRIA, H. de Q.; OLIVEIRA, D. de S. Manejo alimentar de caprinos e ovinos nos trópicos. Veterinária e Zootecnia, v. 23, n. 3, p. 326-346, set. 2016.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | AMARAL JÚNIOR, J. M. DO; MORAIS, E. DE; CARMO, E. S. N. DO; SOUSA, M. A. P. DE; SILVA, A. G. M. E; SILVA, B. A. DA; MARTORANO, L. G.; BERNDT, A. Emissão de metano entérico em búfalas suplementadas com torta de palmiste no Bioma Amazônia. In: SOTTA, E. D.; SAMPAIO, F. G.; COSTA, M. de S. N. (org.). Coletânea de fatores de emissão e remoção de gases de efeito estufa da pecuária brasileira. Brasília, DF: MAPA: SENAR, 2020. p.92-93.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 8. | | AMARAL JÚNIOR, J. M. do; MORAIS, E. de; CARMO, E. S. N. do; SOUSA, M. A. P. de; SILVA, B. A. da; MARTORANO, L. G.; BERNDT, A.; SILVA, A. G. M. e. Enteric methane emission of female buffaloes supplemented with palm kernel cake in the Amazon biome. In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. p. 53-57. (Embrapa Gado de Corte. Documentos, 216). Coordenador Roberto Giolo de Almeida. II SIGEE.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | MONTEIRO, S. do N.; AMARAL JÚNIOR, J. M. do; SOUSA, M. A. P. de; CASTRO, V. C. G. de; CARMO, E. S. N. do; MORAIS, E. de; NAHUM, B. de S.; MARTORANO, L. G. Infrared thermography in the assessment of thermal comfort of confined water Buffaloes in the Amazon Biome. In: INTERNATIONAL SYMPOSIUM ON GREENHOUSE GASES IN AGRICULTURE, 2., 2016, Campo Grande, MS. Proceedings... Brasília, DF: Embrapa, 2016. p. 290-294.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | MORAIS, M. dos S.; DOMICIANO, T. A. de O.; MORAIS, E. de; MIRANDA, A. S.; PASSOS, L. T.; CORRÊA, R. P.; PANTOJA, W. L. da C.; NAHUM, B. S.; SILVA, A. G. M. e; LOURENÇO JUNIOR, J. de B. Avaliação do comportamento ingestivo em búfalas lactantes suplementadas com ração à base de resíduos industriais: cupuaçu e murumuru. In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém, PA. Anais... Belém, PA: Associação Brasileira de Buiatria, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | AMARAL JUNIOR, J. M. do; BRCKO, C. C.; MORAIS, E. de; MARTORANO, L. G.; NAHUM, B. de S.; LOURENÇO JUNIOR, J. de B.; RÊGO, A. C. do; SILVA, A. G. M. e. Desempenho de fêmeas bubalinas suplementadas com torta de palmiste mantidas em sistema de lotação intermitente. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 24., 2014, Vitória. A zootecnia fazendo o Brasil crescer: anais... Vitória: Universidade Federal do Espírito Santo, 2014.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | MORAIS, E. de; CARVALHO, I. C. de; PANTOJA, W. L. da C.; LIMA, A. C. S.; OLIVEIRA, M. E. C.; GUIMARÃES, C. M. C.; LOURENÇO JUNIOR, J. de B.; SILVA, A. G. M. e. Óleo de palma (Elaeis Guineenses Jacq.) na alimentação de ovinos, desaparecimento ruminal da matéria seca, matéria orgânica e proteína bruta. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 24., 2014, Vitória. A zootecnia fazendo o Brasil crescer: anais... Vitória: Universidade Federal do Espírito Santo, 2014.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | MORAIS, M. dos S.; DOMICIANO, T. A. de O.; MIRANDA, A. S.; MORAIS, E. de; PASSOS, L. T.; NERES, L. de S.; LIMA, S. C. G. de; NAHUM, B. S.; SILVA, A. G. M. e; LOURENÇO JUNIOR, J. de B. Produção de leite e consumo de concentrado em búfalas lactantes suplementadas com concentrado à base de torta de cupuaçu (Theobroma grandiflorum) e torta de murumuru (Astrocaryum murumuru). In: CONGRESSO BRASILEIRO DE BUIATRIA, 10.; SEMANA DO MÉDICO VETERINÁRIO DO PARÁ, 37.; SIMPÓSIO PARAENSE DE MEDICINA VETERINÁRIA, 5., 2013, Belém, PA. Anais... Belém, PA: Associação Brasileira de Buiatria, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | AMARAL-JÚNIOR, J. M. do; MORAIS, E. de; LIMA, A. C. S.; MARTORANO, L. G.; NAHUM, B. de S.; SOUSA, L. F.; LOURENÇO-JUNIOR, J. de B.; RODRIGUES, T. C. G. de C.; SILVA, J. A. R. da; SILVA, A. L. da C.; SILVA, A. G. N. e. Effect of palm kernel cake supplementation on voluntary feed intake, in situ rumen degradability and performance in Buffaloes in the Eastern Amazon. Animals, v. 13, n. 5, Article 934, 2023.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 15 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
21/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
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Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
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Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
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DOI: |
10.5433/1679-0359.2017v38n6p3613 |
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Idioma: |
Inglês |
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Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
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Palavras-Chave: |
Powdered Coconut Water. |
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Thesaurus NAL: |
cryopreservation; Lippia. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
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Marc: |
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245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource]
260 $c2017
520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil.
650 $acryopreservation
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653 $aPowdered Coconut Water
700 1 $aMIRANDA, M. dos S.
700 1 $aSILVA, L. K. X.
700 1 $aSILVA, A. O. A. da
700 1 $aSOUSA, J. S. de
700 1 $aMORAIS, E. de
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773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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