| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
06/07/2017 |
|
Data da última atualização: |
21/11/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ROSSI JUNIOR, O. D.; AVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
|
Afiliação: |
Lucas José Luduverio Pizauro, UNESP; Camila Chioda de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Oswaldo Durival Rossi Junior, UNESP; Fernando Antonio de Avila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
|
Título: |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Veterinary Microbiology, v. 204, p. 8-14, 2017. |
|
ISSN: |
0378-1135 |
|
DOI: |
https://doi.org/10.1016/j.vetmic.2017.03.036 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization?time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species. |
|
Palavras-Chave: |
CydB qPCR; Staphylococcus agnetis. |
|
Thesaurus Nal: |
milk; Staphylococcus hyicus. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02359naa a2200277 a 4500
001 2072032
005 2017-11-21
008 2017 bl uuuu u00u1 u #d
022 $a0378-1135
024 7 $ahttps://doi.org/10.1016/j.vetmic.2017.03.036$2DOI
100 1 $aPIZAURO, L. J. L.
245 $aSpecies level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR.$h[electronic resource]
260 $c2017
520 $aIncorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization?time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species.
650 $amilk
650 $aStaphylococcus hyicus
653 $aCydB qPCR
653 $aStaphylococcus agnetis
700 1 $aALMEIDA, C. C. de
700 1 $aSOLTES, G. A.
700 1 $aSLAVIC, D.
700 1 $aROSSI JUNIOR, O. D.
700 1 $aAVILA, F. A. de
700 1 $aZAFALON, L. F.
700 1 $aMACINNES, J. I.
773 $tVeterinary Microbiology$gv. 204, p. 8-14, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 3 | |
| 2. |  | MORAES, P. V. D. de; REIS, J. C. L.; MONKS, P. L.; AZAMBUJA, I. H. V.; SIEWERDT, L. margem bruta de sucessão de culturas usadas para controle do capim-annoni-2 (Eragrostis plana Nees) Pesquisa agropecuária gaúcha, Porto Alegre, v. 14, n. 2, p. 135-141, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Clima Temperado. |
|    |
| 3. | | ROSSI, P.; RUTZ, F.; LIMA, G. J. M. M. de; NUNES, J. K.; ANCIUTI, M. A.; MORAES, P. V. D. de; SILVA, A. G. C. da; SILVEIRA, M. H. D. da; MAIER, J. C. Efeito do adsorvente a base de glucomamano esterificado no desempenho e caracterização visceral de frangos de corte. Revista Brasileira de Agrociência, Pelotas, v. 16, n. 1-4, p. 91-100, 2010. Projeto: 11.11.11.111.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Suínos e Aves. |
| |
| Registros recuperados : 3 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
