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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/01/2017 |
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Data da última atualização: |
20/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, L. F. dos; SILVA, R. J. S.; AMARAL, D. O. J. do; PAULA, M. F. B. de; FALCÃO, L. L.; LEGAVRE, T.; ALVES, R. M.; MARCELLINO, L. H.; MICHELI, F. |
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Afiliação: |
Lucas Ferraz dos Santos, Universidade Estadual de Santa Cruz; Raner José Santana Silva, Universidade Estadual de Santa Cruz; Daniel Oliveira Jordão do Amaral, Universidade Estadual de Santa Cruz; Márcia Fabiana Barbosa de Paula, Universidade Estadual de Santa Cruz; LOENI LUDKE FALCAO, Cenargen; Thierry Legavre, CIRAD; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; Fabienne Micheli, Universidade Estadual de Santa Cruz / CIRAD. |
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Título: |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 8, e0160646, 2016. |
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DOI: |
http://dx.doi.org/10.1371/journal.pone.0160646 |
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Idioma: |
Inglês |
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Conteúdo: |
Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality. MenosCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results wi... Mostrar Tudo |
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Palavras-Chave: |
Genotipos. |
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Thesagro: |
Cupuaçu. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153764/1/document-4.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500
001 2060964
005 2022-05-20
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1371/journal.pone.0160646$2DOI
100 1 $aSANTOS, L. F. dos
245 $aSelection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum.$h[electronic resource]
260 $c2016
520 $aCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality.
650 $aCupuaçu
653 $aGenotipos
700 1 $aSILVA, R. J. S.
700 1 $aAMARAL, D. O. J. do
700 1 $aPAULA, M. F. B. de
700 1 $aFALCÃO, L. L.
700 1 $aLEGAVRE, T.
700 1 $aALVES, R. M.
700 1 $aMARCELLINO, L. H.
700 1 $aMICHELI, F.
773 $tPlos One$gv. 11, n. 8, e0160646, 2016.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
06/08/2019 |
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Data da última atualização: |
07/08/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MORAES, E. de; PIENIZ, L. C.; ITALIANO, E. C.; TEIXEIRA, L. B. |
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Afiliação: |
ERCI DE MORAES, UEPAE de Manaus; LUIZ CARLOS PIENIZ, UEPAE de Manaus; EDSON CÂMARA ITALIANO, UEPAE de Manaus; LEOPOLDO BRITO TEIXEIRA, UEPAE de Manaus. |
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Título: |
Comportamento de mestiços Holando/Zebu em terra firme no Estado do Amazonas. |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 1.; REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 17., 1980, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Zootecnia, 1980. |
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Páginas: |
p. 236. |
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Idioma: |
Português |
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Thesagro: |
Rebanho. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200406/1/Id-4139-pg-236.pdf
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Marc: |
LEADER 00582nam a2200157 a 4500
001 2111155
005 2019-08-07
008 1980 bl uuuu u00u1 u #d
100 1 $aMORAES, E. de
245 $aComportamento de mestiços Holando/Zebu em terra firme no Estado do Amazonas.
260 $aIn: CONGRESSO BRASILEIRO DE ZOOTECNIA, 1.; REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 17., 1980, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Zootecnia$c1980
300 $ap. 236.
650 $aRebanho
700 1 $aPIENIZ, L. C.
700 1 $aITALIANO, E. C.
700 1 $aTEIXEIRA, L. B.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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