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| Registros recuperados : 13 | |
| 7. |  | ELAD, Y.; FREEMAN, S.; MONTE, E. (ed.). Biocontrol Agents: Mode of Action and Interaction with other Means of Control. Working Group Biological Control of Fungal and Bacterial Plant Pathogens: Proceedings of the meeting at Sevilla (Spain), 30 November - 3 December, 2000. A Dijon: International Organization for Biological and Integrated Control of Noxious Animals and Plants, West Palaearctic Regional Section (IOBC/WPRS), 2001. 376 p. (IOBC/WPRS Bulletin, v. 24, n. 3, 2001).| Biblioteca(s): Embrapa Meio Ambiente. |
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| 10. | | PÉREZ, E.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; BETTIOL, W.; MONTE, E.; HERMOSA, R. The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei. Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 1181. 14 p.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | RUBIO, M. B.; VICENTE, R.; BETTIOL, W.; MORCUENDE, R.; MONTE, E.; HERMOSA, R. Tomato plant responses to salt stress after combined application of Trichoderma harzianum and chemical fertilization. In: INTERNATIONAL TRICHODERMA & GLIOCADIUM WORKSHOP, 15., 2018, Salamanca. Opportunism and conversations in the environment: proceedings. Salamanca: University of Salamanca, 2018. p. 67.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | RUBIO, M. B.; HERMOSA, R.; VICENTE, R.; GOMÉZ-ACOSTA, F. E.; MORCUENDE, R.; MONTE, E.; BETTIOL, W. The combination of trichoderma harzianum and chemical fertilization leads to the deregulation of phytohormone networking, preventing the adaptive responses of tomato plants to salt stress. Frontiers in Plant Science, v. 8, p. 1-14, 2017. Article 294.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | DOMINGUEZ, S.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; NICOLÁS, C.; BETTIOL, W.; HERMOSA, R.; MONTE, E. Nitrogen metabolism and growth enhancement in tomato plants challenged with Trichoderma harzianum expressing the Aspergillus nidulans acetamidase amdS gene. Frontiers in Microbiology, v. 7, article 1182, 2016.| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/01/2016 |
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Data da última atualização: |
25/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PÉREZ, E.; RUBIO, M. B.; CARDOZA, R. E.; GUTIÉRREZ, S.; BETTIOL, W.; MONTE, E.; HERMOSA, R. |
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Afiliação: |
ESCLAUDYS PEREZ, Universidad de Salamanca; MARIA BELEN RUBIO, Universidad de Salamanca; ROSA ELENA CARDOSA, Universidad de León; SANTIAGO GUTIERREZ, Universidad de León; WAGNER BETTIOL, CNPMA; ENRIQUE MONTE, Universidad de Salamanca; ROSA HERMOSA, Universidad de Salamanca. |
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Título: |
The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 1181. |
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Páginas: |
14 p. |
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ISBN: |
http://dx.doi.org/10.3389/fmicb.2015.01181 |
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Idioma: |
Inglês |
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Conteúdo: |
Species of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato plant responses was examined in greenhouse assays. The growth of plants colonized by the silenced transformants was reduced and the plants exhibited an increased susceptibility to B. cinerea in comparison with the responses observed for control plants. In addition, the plants turned yellowish and were defective in jasmonic acid- and ethylene-regulated signaling pathways which was seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes. MenosSpecies of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato p... Mostrar Tudo |
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Palavras-Chave: |
2-phenylethanol; Tparo7 gene; Tyrosol. |
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Thesagro: |
Controle biológico; Trichoderma. |
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Thesaurus NAL: |
Antifungal agents; Gene silencing; Phenylethyl alcohol; Salicylic acid; Shikimate pathway. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136478/1/2015AP28.pdf
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|
Marc: |
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100 1 $aPÉREZ, E.
245 $aThe importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei.$h[electronic resource]
260 $c2015
300 $a14 p.
520 $aSpecies of Trichoderma exert direct biocontrol activity against soil-borne plant pathogens due to their ability to compete for nutrients and to inhibit or kill their targets through the production of antibiotics and/or hydrolytic enzymes. In addition to these abilities, Trichoderma spp. have beneficial effects for plants, including the stimulation of defenses and the promotion of growth. Here we study the role in biocontrol of the T. parareesei Tparo7 gene, encoding a chorismate mutase (CM), a shikimate pathway branch point leading to the production of aromatic amino acids, which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites. We isolated T. parareesei transformants with the Tparo7 gene silenced. Compared with the wild-type, decreased levels of Tparo7 expression in the silenced transformants were accompanied by reduced CM activity, lower growth rates on different culture media, and reduced mycoparasitic behavior against the phytopathogenic fungi Rhizoctonia solani, Fusarium oxysporum and Botrytis cinerea in dual cultures. By contrast, higher amounts of the aromatic metabolites tyrosol, 2-phenylethanol and salicylic acid were detected in supernatants from the silenced transformants, which were able to inhibit the growth of F. oxysporum and B. cinerea. In in vitro plant assays, Tparo7-silenced transformants also showed a reduced capacity to colonize tomato roots. The effect of Tparo7-silencing on tomato plant responses was examined in greenhouse assays. The growth of plants colonized by the silenced transformants was reduced and the plants exhibited an increased susceptibility to B. cinerea in comparison with the responses observed for control plants. In addition, the plants turned yellowish and were defective in jasmonic acid- and ethylene-regulated signaling pathways which was seen by expression analysis of lipoxygenase 1 (LOX1), ethylene-insensitive protein 2 (EIN2) and pathogenesis-related protein 1 (PR-1) genes.
650 $aAntifungal agents
650 $aGene silencing
650 $aPhenylethyl alcohol
650 $aSalicylic acid
650 $aShikimate pathway
650 $aControle biológico
650 $aTrichoderma
653 $a2-phenylethanol
653 $aTparo7 gene
653 $aTyrosol
700 1 $aRUBIO, M. B.
700 1 $aCARDOZA, R. E.
700 1 $aGUTIÉRREZ, S.
700 1 $aBETTIOL, W.
700 1 $aMONTE, E.
700 1 $aHERMOSA, R.
773 $tFrontiers in Microbiology, Lausanne$gv. 6, 2015. Article 1181.
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