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| Registros recuperados : 55 | |
| 41. |  | JOAQUIM, P. I. L.; MOLINARI, M. D. C.; MARIN, S. R. R.; BARBOSA, D. A.; VIANA, A. J. C.; RECH FILHO, E. L.; HENNING, F. A.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Nitrogen compounds transporters: candidates to increase the protein content in soybean seeds. Journal of Plant Interactions, v. 17, n. 1, p. 309-318, 2022. Na publicação: Elibio L. Rech.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 42. | | JOAQUIM, P. I. L.; MOLINARI, M. D. C.; MARIN, S. R. R.; BARBOSA, D. A.; VIANA, A. J. C.; RECH, E. L.; HENNING, F. A.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Nitrogen compounds transporters: candidates to increase the protein content in soybean seeds. Journal of Plant Interactions, v. 17, n. 1, p. 309-318, 2022.| Biblioteca(s): Embrapa Soja. |
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| 43. | | MOLINARI, M. D. C.; KANAMORI, N.; FUGANTI-PAGLIARINI, R.; MARIN, S. R.; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; NEUMAIER, N.; URANO, K.; SHINOZAKI, K.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; NEPOMUCENO, A. L. Obtenção e análise molecular da cultivar de soja BRS 184 geneticamente modificada com a construção SAT6 visando tolerância a seca. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 44. | | MOLINARI, M. D. C.; BARBOSA, D. A.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; CARNEIRO, E. A.; QUEIROZ, A. A.; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Número de cópias e padrão de segregação em soja geneticamente modificada via Agrobacterium. In: REUNIÃO DE PESQUISA DE SOJA, 36., 2017, Londrina, PR. Resumos expandidos... Londrina: Embrapa Soja, 2017. p. 179-181. (Embrapa Soja. Documentos, 388). Editado por Alvadi Antonio Balbinot Junior, Fernando Augusto Henning, Regina Maria Villas Bôas de Campos Leite.| Biblioteca(s): Embrapa Soja. |
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| 45. | | COSTA, E. A.; ANONI, C. O.; MANCINI, M. C.; SANTOS, F. R. C.; MARCONI, T. G.; GAZAFFI, R.; PASTINA, M. M.; PERECIN, D.; MOLINARI, M.; XAVIER, M. A.; PINTO, L. R.; SOUZA, A. P.; GARCIA, A. A. F. QTL mapping including codominant SNP markers with ploidy level information in a sugarcane progeny. Euphytica, Dordrecht, v. 211, n. 1, p. 1-16, 2016.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 46. | | MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; BARBOSA, D. de A.; KAFER, J. M.; MARIN, D. C.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L.; RECH FILHO, E. L. Screening de proteínas bioativas com potencial antioxidante em proteoma de soja cv. BRS 537. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 154. p. 174.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 47. | | MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; BARBOSA, D. de A.; KAFER, J. M.; MARIN, D. C.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L.; RECH FILHO, E. L. Screening de proteínas bioativas com potencial antioxidante em proteoma de soja cv. BRS 537. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 154. p. 174.| Biblioteca(s): Embrapa Soja. |
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| 48. | | CARANHATO, A. L. H.; BRAGA, P.; BARBOSA, D. A.; MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; OLIVEIRA, M. F.; MERTZ-HENNING, L. M.; MELO, C. L. P.; NEPOMUCENO, A. L. Respostas fisiológicas para a tolerância a seca em diferentes genótipos de soja obtidos a partir da introgressão da construção 35S:AtAREB1FL. In: CONGRESSO BRASILEIRO DE SOJA, 8., 2018, Goiânia. Inovação, tecnologias digitais e sustentabilidade da soja: anais. Brasília, DF: Embrapa, 2018. P. 700-702.| Biblioteca(s): Embrapa Soja. |
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| 49. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. Plos One, v. 10, n. 9, e0139051, 2015. 16 p.| Biblioteca(s): Embrapa Agroenergia; Embrapa Soja. |
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| 50. | | FUGANTI-PAGLIARINI, R; FERREIRA, L. C.; RODRIGUES, F. A.; MOLINARI, H. B. C.; MARIN, S. R. R.; MOLINARI, M. D. C.; MARCOLINO-GOMES, J.; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; NEUMAIER, N.; KANAMORI, N.; FUJITA, Y.; MIZOI, J.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Characterization of soybean genetically modified for drought tolerance in field conditions. Frontiers in Plant Science, v. 8, artigo 448, 2017.| Biblioteca(s): Embrapa Agroenergia; Embrapa Soja. |
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| 51. | | FUGANTI-PAGLIARINI, R.; CARVALHO, J. F. C.; ROLLA-SANTOS, A. A. P.; ENGELS, C.; MOLINARI, M. D. C.; VASQUEZ, G.; MARIN, S. R. R.; KANAMORI, N.; FARIAS, J. R. B.; NEUMAIER, N.; OLIVEIRA, M. C. N.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Análise fisiológica e agronômica de plantas de soja geneticamente modificadas com os genes AtDREB1A e AtDREB2A sob déficit hídrico em condições experimentais de campo. In: CONGRESO DE LA SOJA DEL MERCOSUR, 5.; FORO DE LA SOJA ASIA, 1., 2011, Rosário. Un grano: un universo. [Rosário: Asociación de la Cadena de la Soja Argentina], 2011. 7 p. 1 CD-ROM. MERCOSOJA 2011.| Biblioteca(s): Embrapa Soja. |
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| 52. | | FUGANTI, R.; ENGELS, C.; RODRIGUES, F. A.; MARINHO, J. P.; LEITE, J. P.; GIROTTO, L.; MOLINARI, M. D. C.; KANAMORI, N.; MARIN, S. R. R.; FARIAS, J. R. B.; NEUMAIER, N.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; MIZOI, J.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Molecular characterization by qPCR of Soybean (Glycine max L. Merril) genetically modified lines expressing AtDREB2A gene, submitted to water stress treatments. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 188-189.| Biblioteca(s): Embrapa Soja. |
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| 53. | | HONNA, P. T.; FUGANTI-PAGLIARINI, R.; FERREIRA, L. C.; MOLINARI, M. D. C.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEUMAIER, N.; MERTZ-HENNING, L. M.; KANAMORI, N.; NAKASHIMA, K.; TAKASAKI, H.; URANO, K.; SHINOZAKI, K.; YAMAGUCHI-SHINOZAKI, K.; DESIDÉRIO, J. A.; NEPOMUCENO, A. L. Molecular, physiological, and agronomical characterization, in greenhouse and in field conditions, of soybean plants genetically modified with AtGolS2 gene for drought tolerance. Molecular Breeding, v. 36, n. 11, nov. 2016.| Biblioteca(s): Embrapa Soja. |
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| 54. | | MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; FERREIRA, L. C.; BARBOSA, D. de A.; MARCOLINO-GOMES, J.; OLIVEIRA, M. C. N. de; MERTZ-HENNING, L. M.; KANAMORI, N.; TAKASAKI, H.; URANO, K.; SHINOZAKI, K.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. Overexpression of AtNCED3 gene improved drought tolerance in soybean in greenhouse and field conditions Genetics and Molecular Biology, v. 43, n. 3, e20190292, 2020. 12 p.| Biblioteca(s): Embrapa Soja. |
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| 55. | | MARINHO, J. P.; PAGLIARINI, R. F.; MOLINARI, M. D. C.; MARCOLINO-GOMES, J.; CARANHOTO, A. L. H.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; FOLONI, J. S. S.; MELO, C. L. P. de; KIDOKORO, S.; MIZOI, J.; KANAMORI, N.; YAMAGUCHI-SHINOZAKI, K.; NAKASHIMA, K.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Overexpression of full-length and partial DREB2A enhances soybean drought tolerance. AGRONOMY SCIENCE AND BIOTECHNOLOGY, v. 8, 2022. 21 p.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 55 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
02/10/2015 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
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Páginas: |
16 p. |
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DOI: |
10.1371/journal.pone.0139051 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
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Palavras-Chave: |
Drought stress; Soybean. |
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Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
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Thesaurus NAL: |
Drought tolerance; Soybeans; Transcriptome. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
LEADER 02592naa a2200361 a 4500
001 2079599
005 2017-11-13
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0139051$2DOI
100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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