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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/09/2021 |
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Data da última atualização: |
23/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, M. dos S.; SOARES, T. L.; COSTA, E. M. R.; SILVA, R. L. da; JESUS, O. N. de; JUNGHANS, T. G.; SOUZA, F. V. D. |
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Afiliação: |
MICHELE DOS SANTOS FERREIRA, UFRB; TALIANE LEILA SOARES, UEFS; EVA MARIA RODRIGUES COSTA, FAMAM; RONILZE LEITE DA SILVA; ONILDO NUNES DE JESUS, CNPMF; TATIANA GOES JUNGHANS, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Optimization of culture medium for the in vitro germination and histochemical analysis of Passiflora spp. pollen grains. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Scientia Horticulturae, v. 288, 2021. |
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ISSN: |
0304-4238 |
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Idioma: |
Inglês |
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Conteúdo: |
Pollen viability studies are essential for high production of fruits and seeds, as well as for genetic improvement and conservation strategies. The objective of this work was to investigate the most suitable culture medium for in vitro germination of pollen grains and pollen tube growth of 11 wild Passiflora species and to examine the viability by means of histochemical testing with 2% Alexander?s solution. This is the first study to provide data on the pollen tube growth of passion fruit. For in vitro germination, two culture media were used, BK and SM, both supplemented with different concentrations of sucrose (5, 10, 15, 20, 25, 30 and 35%). The experimental design was completely randomized in an 11×7 × 2 factorial scheme (species x sucrose concentrations x culture media). Sucrose influenced the germination of pollen grains and the pollen tube growth. P. edmundoi had the highest germination rate (89%) and pollen tube length (2.6 mm) in SM culture medium supplemented with 30% and 15% sucrose, respectively. Regarding the histochemical analysis, all the Passiflora species presented high percentage of viable pollen grains (above 79%). The information obtained in this study allowed identifying the most suitable culture medium for each Passiflora species, helping in the choice of male parents with high potential for fertility, to maximize the potential success of controlled crosses in genetic improvement programs. The results can also support studies of the conservation of pollen grains. MenosPollen viability studies are essential for high production of fruits and seeds, as well as for genetic improvement and conservation strategies. The objective of this work was to investigate the most suitable culture medium for in vitro germination of pollen grains and pollen tube growth of 11 wild Passiflora species and to examine the viability by means of histochemical testing with 2% Alexander?s solution. This is the first study to provide data on the pollen tube growth of passion fruit. For in vitro germination, two culture media were used, BK and SM, both supplemented with different concentrations of sucrose (5, 10, 15, 20, 25, 30 and 35%). The experimental design was completely randomized in an 11×7 × 2 factorial scheme (species x sucrose concentrations x culture media). Sucrose influenced the germination of pollen grains and the pollen tube growth. P. edmundoi had the highest germination rate (89%) and pollen tube length (2.6 mm) in SM culture medium supplemented with 30% and 15% sucrose, respectively. Regarding the histochemical analysis, all the Passiflora species presented high percentage of viable pollen grains (above 79%). The information obtained in this study allowed identifying the most suitable culture medium for each Passiflora species, helping in the choice of male parents with high potential for fertility, to maximize the potential success of controlled crosses in genetic improvement programs. The results can also support studies of the conservation of poll... Mostrar Tudo |
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Thesagro: |
Maracujá. |
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Categoria do assunto: |
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Marc: |
LEADER 02163naa a2200217 a 4500
001 2134731
005 2021-09-23
008 2021 bl uuuu u00u1 u #d
022 $a0304-4238
100 1 $aFERREIRA, M. dos S.
245 $aOptimization of culture medium for the in vitro germination and histochemical analysis of Passiflora spp. pollen grains.$h[electronic resource]
260 $c2021
520 $aPollen viability studies are essential for high production of fruits and seeds, as well as for genetic improvement and conservation strategies. The objective of this work was to investigate the most suitable culture medium for in vitro germination of pollen grains and pollen tube growth of 11 wild Passiflora species and to examine the viability by means of histochemical testing with 2% Alexander?s solution. This is the first study to provide data on the pollen tube growth of passion fruit. For in vitro germination, two culture media were used, BK and SM, both supplemented with different concentrations of sucrose (5, 10, 15, 20, 25, 30 and 35%). The experimental design was completely randomized in an 11×7 × 2 factorial scheme (species x sucrose concentrations x culture media). Sucrose influenced the germination of pollen grains and the pollen tube growth. P. edmundoi had the highest germination rate (89%) and pollen tube length (2.6 mm) in SM culture medium supplemented with 30% and 15% sucrose, respectively. Regarding the histochemical analysis, all the Passiflora species presented high percentage of viable pollen grains (above 79%). The information obtained in this study allowed identifying the most suitable culture medium for each Passiflora species, helping in the choice of male parents with high potential for fertility, to maximize the potential success of controlled crosses in genetic improvement programs. The results can also support studies of the conservation of pollen grains.
650 $aMaracujá
700 1 $aSOARES, T. L.
700 1 $aCOSTA, E. M. R.
700 1 $aSILVA, R. L. da
700 1 $aJESUS, O. N. de
700 1 $aJUNGHANS, T. G.
700 1 $aSOUZA, F. V. D.
773 $tScientia Horticulturae$gv. 288, 2021.
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