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Registro Completo |
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Biblioteca(s): |
Embrapa Soja; Embrapa Trigo. |
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Data corrente: |
26/02/2021 |
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Data da última atualização: |
06/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARPENTIERI-PIPOLO, V.; BARRETO, T. P.; SILVA, D. A. da; ABDELNOOR, R. V.; MARIN, S. R. R.; DEGRASSI, G. |
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Afiliação: |
VALERIA CARPENTIERI PIPOLO, CNPT; THALES PEREIRA BARRETO, (2) Syngenta Proteção de Cultivos LTDA. São Paulo, SP. E-mail: thalepbarreto@yahoo.com.br; DAIANA ALVES DA SILVA, (3) Agronomic Institute (IAC) - Center for Grain and Fiber - Zip Code 13075-630 – Campinas, SP –Brazil, E-mail: daiagrouel2002@hotmail.com; RICARDO VILELA ABDELNOOR, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; GIULIANO DEGRASSI, (6) International Centre for Genetic Engineering and Biotechnology (ICGEB), 1Industrial Biotechnology Group, Parque Tecnolo?gico Miguelete, San Marti?n, Buenos Aires, Repu?blica Argentina. E-mail: degrassi@icgeb.org. |
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Título: |
Soybean improvement for lipoxygenase-free by simple sequence repeat (SSR) markers selection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Botanical Research, v. 3, n.1, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Beany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified. The SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in the F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality. Index terms: Glycine max, lipoxygenase isozymes, molecular markers, MAS |
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Palavras-Chave: |
Lipoxigenase; Lipoxygenase isozymes; Market-assisted selection (MAS); MAS; Molecular markers; Simple sequence repeat (SSR). |
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Thesagro: |
Glycine Max; Isoenzima; Marcador Molecular; Soja. |
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Thesaurus Nal: |
Genetic markers; Isozymes; Lipoxygenase; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228513/1/Soybean-Improvement-for-lipoxygenase-free-2818-12720-1-PB-2021.pdf
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Marc: |
LEADER 02378naa a2200349 a 4500
001 2130306
005 2021-12-06
008 2021 bl uuuu u00u1 u #d
100 1 $aCARPENTIERI-PIPOLO, V.
245 $aSoybean improvement for lipoxygenase-free by simple sequence repeat (SSR) markers selection.$h[electronic resource]
260 $c2021
520 $aBeany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified. The SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in the F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality. Index terms: Glycine max, lipoxygenase isozymes, molecular markers, MAS
650 $aGenetic markers
650 $aIsozymes
650 $aLipoxygenase
650 $aSoybeans
650 $aGlycine Max
650 $aIsoenzima
650 $aMarcador Molecular
650 $aSoja
653 $aLipoxigenase
653 $aLipoxygenase isozymes
653 $aMarket-assisted selection (MAS)
653 $aMAS
653 $aMolecular markers
653 $aSimple sequence repeat (SSR)
700 1 $aBARRETO, T. P.
700 1 $aSILVA, D. A. da
700 1 $aABDELNOOR, R. V.
700 1 $aMARIN, S. R. R.
700 1 $aDEGRASSI, G.
773 $tJournal of Botanical Research$gv. 3, n.1, 2021.
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Registro original: |
Embrapa Trigo (CNPT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Clima Temperado. Para informações adicionais entre em contato com cpact.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
21/02/2014 |
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Data da última atualização: |
06/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BUSTAMANTE, F. O.; ROCHA, L. C.; TORRES, G. A.; DAVIDE, L. C.; MITTELMANN, A. |
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Afiliação: |
Fernanda O. Bustamante; Laiane C. Rocha; Giovana A. Torres; Lisete C. Davide; ANDREA MITTELMANN, CNPGL. |
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Título: |
Distribution of rDNA in Diploid and Polyploid Lolium multiflorum Lam. and Fragile Sites in 45S rDNA Regions. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Crop Science, v. 54, p. 1-9, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The distribution, number, and location of ribosome DNA (rDNA) regions on chromosomes were evaluated, and transcriptional activity was described in genotypes of Italian ryegrass (Lolium multiflorum Lam.), as well as in an offspring resulting from interbreeding. Nucleoli and nucleolar organizer regions were labeled with silver nitrate and fluorescent in situ hybridization (FISH) was performed with 5S and 45S rDNA as probes. Hybridization procedures were performed on slides previously stained with the Ag-NOR method. The 5S rDNA sites were highly conserved, while 45S rDNA sites had wide variability, even showing more than one site on the same chromosome. One of the genotypes had more than expected 45S rDNA sites. Approximately 95% of the cells at metaphases had at least one chromosome break/gap in the 45S rDNA site, resulting in chromosome fragmentation. Thus, 45S rDNA correspond to fragile sites in L. multiflorum. These events can affect genome organization and cause new chromosomal rearrangements which, along with some other factors, might be responsible for microevolutionary changes involved in differentiation and speciation. Not all 45S rDNA sites are transcriptionally active. Variation in both the number and size of nucleoli and mechanisms of nucleolar fusion were observed in L. multiflorum. |
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Thesagro: |
DNA. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01846naa a2200181 a 4500
001 1980839
005 2015-02-06
008 2014 bl uuuu u00u1 u #d
100 1 $aBUSTAMANTE, F. O.
245 $aDistribution of rDNA in Diploid and Polyploid Lolium multiflorum Lam. and Fragile Sites in 45S rDNA Regions.$h[electronic resource]
260 $c2014
520 $aThe distribution, number, and location of ribosome DNA (rDNA) regions on chromosomes were evaluated, and transcriptional activity was described in genotypes of Italian ryegrass (Lolium multiflorum Lam.), as well as in an offspring resulting from interbreeding. Nucleoli and nucleolar organizer regions were labeled with silver nitrate and fluorescent in situ hybridization (FISH) was performed with 5S and 45S rDNA as probes. Hybridization procedures were performed on slides previously stained with the Ag-NOR method. The 5S rDNA sites were highly conserved, while 45S rDNA sites had wide variability, even showing more than one site on the same chromosome. One of the genotypes had more than expected 45S rDNA sites. Approximately 95% of the cells at metaphases had at least one chromosome break/gap in the 45S rDNA site, resulting in chromosome fragmentation. Thus, 45S rDNA correspond to fragile sites in L. multiflorum. These events can affect genome organization and cause new chromosomal rearrangements which, along with some other factors, might be responsible for microevolutionary changes involved in differentiation and speciation. Not all 45S rDNA sites are transcriptionally active. Variation in both the number and size of nucleoli and mechanisms of nucleolar fusion were observed in L. multiflorum.
650 $aDNA
700 1 $aROCHA, L. C.
700 1 $aTORRES, G. A.
700 1 $aDAVIDE, L. C.
700 1 $aMITTELMANN, A.
773 $tCrop Science$gv. 54, p. 1-9, 2014.
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Embrapa Clima Temperado (CPACT) |
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