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| Registros recuperados : 34 | |
| 3. |  | ROCHA, N. A. S.; LEÃO, B. C. S.; NOGUEIRA, E.; MINGOTI, G. Z. Bovine embryos cultured in vitro in the presence of antioxidants: implications for blastocysts development, quality and cryotolerance. In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 4. Animal Reproduction, v.9, n.4, p.829, 2012. (ISABR 2012).| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | ROCHA, N. A. S.; LEÃO, B. C. S.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Levels of reactive oxygen species (Ros), apoptosis and cryoresistance of bovine embryos culture in the presence of antioxidants. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012.| Biblioteca(s): Embrapa Pantanal. |
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| 7. | | ROCHA, N. A. S.; LEÃO, B. C. S.; NOGUEIRA, E.; MINGOTI, G. Z. Supplementation with antioxidants during maturation increases resistance to oxidative stress in bovine embryos pruduced in vitro but does not improve cryotolerance. Animal Reproduction, v. 9. n. 3. p. 681, jul/set, 2012. Publicado no: Proceedings of the 26th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August 30th to September 2nd, 2012, Foz do Iguaçu, PR, Brazil.| Biblioteca(s): Embrapa Pantanal. |
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| 8. | | ROCHA, N. A. de S.; LEÃO, B. C. da S.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Bovine embryo culture in the presence of antioxidants: implications for blastocysts development, cryotolerance and levels of intracellular reactive oxygen species (ROS). Reproduction in Domestic Animals, v. 47, n. 4, p. 531, 2012. Publicado no: Proceedings of the 17th International Congress on Animal Reproduction (ICAR) Vancouver, Canada; 29 July - 2 August 2012.| Biblioteca(s): Embrapa Pantanal. |
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| 9. | | ROCHA, N. A. de S.; LEÃO, B. C. da S.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Cultivo in vitro de embriões bovinos na presença de antioxidantes: implicações sobre o desenvolvimento embrionário, criotolerância e níveis intracelulares de espécies reativas de oxigênio. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012.| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | MENEGHEL, M.; DALL'ACQUA, P. C.; AMBROGI, M.; LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; MINGOTI, G. Z. Lipid content and cryotolerance of in vitro-produced bovine embryos treated with forskolin before vitrification. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 395-400, abril 2017. Título em português: Acúmulo de lipídios intracitoplasmáticos, desenvolvimento e criotolerância de embriões bovinos produzidos in vitro e tratados com diferentes concentrações de forskolin antes da vitrificação.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | NOGUEIRA, E.; SILVA, A. S. da; AMARAL, T. B.; ÍTAVO, L. C. V.; DIAS, A. M.; MINGOTI, G. Z. Follicular dynamics and production of oocytes in young Nellore heifers with energetic supplementation. Revista Brasileira de Zootecnia, v. 41, n. 9, p. 2012-2017, 2012.| Biblioteca(s): Embrapa Pantanal. |
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| 19. | | MINGOTI, G. Z.; JIMENEZ FILHO, D. L.; FERRAUDO, A.; LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; NOGUEIRA, E.; DUTRA, I. S. Analysis of perception of risk factors for public health in the use of hormones in assisted reproduction programs in cattle. Animal Reproduction, v. 13, n. 3, p. 444, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Biblioteca(s): Embrapa Pantanal. |
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| 20. | | MENEGHEL, M.; DALL ACQUA, P. C.; AMBROGI, M.; GOUVÊA, L. M.; ACHILLES, M. A.; LEÃO, B. C. S.; NOGUEIRA, E.; ROCHA-FRIGONI, N. A. S.; MINGOTI, G. Z. Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. Animal Reproduction, v.13, n.3, p.701, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 34 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
23/02/2010 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MINGOTI, G. Z.; CAIADO CASTRO, V. S. D.; NICIURA, S. C. M.; BARRETTO, L. S. S.; GARCIA, J. M. |
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Afiliação: |
G. Z. MINGOTI, UNESP/ARAÇATUBA; V. S. D. CAIADO CASTRO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; L. S. S. BARRETTO, UNESP/JABOTICABAL; J. M. GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos cultured in vitro. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Zygote, v. 17, n. 4, p. 321-328, may 2009. |
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DOI: |
10.1017/S0967199409005450 |
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Idioma: |
Inglês |
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Conteúdo: |
SummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% CO2, 5% O2 and 90% N2 (5% O2)] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O2 and defined media (PVA, PVP and Ficoll) at 5% O2. Independent of macromolecule supplement, IVM at 20% O2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O2 (52.5, 21.8, 18.2 and 15.6%, respectively). This study indicates that BSA, PVA, PVP and Ficoll can replace serum during IVM and that the optimal atmospheric condition for in vitro production of bovine embryos is 5% CO2 and 20% O2. MenosSummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% CO2, 5% O2 and 90% N2 (5% O2)] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O2 and defined media (PVA, PVP and Ficoll) at 5% O2. Independent of macromolecule supplement, IVM at 20% O2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O2 (52.5,... Mostrar Tudo |
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Palavras-Chave: |
Macromolecule. |
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Thesagro: |
Embrião; Gado de corte. |
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Thesaurus NAL: |
Animal breeding; Beef cattle; Oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02494naa a2200253 a 4500
001 1658493
005 2023-03-15
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1017/S0967199409005450$2DOI
100 1 $aMINGOTI, G. Z.
245 $aThe effect of interaction between macromolecule supplement and oxygen tension on bovine oocytes and embryos cultured in vitro.$h[electronic resource]
260 $c2009
520 $aSummaryAiming to improve in vitro production of bovine embryos and to obtain supplements to replace serum for in vitro maturation (IVM), this study evaluated the effects of macromolecular supplementation of IMV medium (bovine serum albumin - BSA, polyvinyl alcohol - PVA, polyvinyl pyrrolidone - PVP, Ficoll, KnockoutSR, or fetal calf serum - FCS) and oxygen tension [5% CO2 in air (20% O2) or 5% CO2, 5% O2 and 90% N2 (5% O2)] on oocyte maturation and embryo development. Nuclear progression to germinal vesicle breakdown, metaphase I and metaphase II stages were evaluated and overall results revealed that undefined (FCS) and semi-defined (BSA) media gave better results at 20% O2 and defined media (PVA, PVP and Ficoll) at 5% O2. Independent of macromolecule supplement, IVM at 20% O2 was considered optimal for nuclear maturation. To evaluate embryo development, oocytes matured in the previously described conditions were fertilized and cultured at the same oxygen tension used for IVM and assessed for cleavage (43.0 to 74.8%) and development to morulae (16.4 to 33.8%), blastocyst (7.7 to 52.9%) and hatched blastocyst (9.6 to 48.1%). Apart from oxygen tension, all treatments, except Knockout (22.7%), gave similar results for blastocyst development (26.5 to 38.7%). Independently of macromolecule supplement, higher development rates were obtained in an oxygen tension of 20% O2 (67.4% cleavage, 29.2% morulae, 40.8% blastocyst and 34.0% hatched blastocyst) when compared with 5% O2 (52.5, 21.8, 18.2 and 15.6%, respectively). This study indicates that BSA, PVA, PVP and Ficoll can replace serum during IVM and that the optimal atmospheric condition for in vitro production of bovine embryos is 5% CO2 and 20% O2.
650 $aAnimal breeding
650 $aBeef cattle
650 $aOocytes
650 $aEmbrião
650 $aGado de corte
653 $aMacromolecule
700 1 $aCAIADO CASTRO, V. S. D.
700 1 $aNICIURA, S. C. M.
700 1 $aBARRETTO, L. S. S.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 17, n. 4, p. 321-328, may 2009.
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