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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
26/10/2011 |
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Data da última atualização: |
26/10/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVARENGA, S. M.; CAIXETA, E. T.; HUFNAGEL, B.; THIEBAUT, F.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; SAKIYAMA, N. S. |
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Afiliação: |
SAMUEL MAZZINGHY ALVARENGA, UFV; EVELINE TEIXEIRA CAIXETA, SAPC; BÁRBARA HUFNAGEL, UFV; FLÁVIA THIEBAUT, UFV; EUNIZE MACIEL ZAMBOLIM, UFV; LAÉRCIO ZAMBOLIM, UFV; NEY SUSSUMU SAKIYAMA, UFV. |
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Título: |
Marcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugem. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira. Brasília, v. 46, n.8, p. 890-898, ago. 2011. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi identificar marcadores moleculares relacionados à resistência do cafeeiro (Coffea arabica) à ferrugem (Hemileia vastatrix). Foram identificadas sequências de DNA potencialmente envolvidas na resistência do cafeeiro a doenças, por meio de análise ?in silico?, a partir das informações geradas pelo Projeto Brasileiro do Genoma Café. A partir das sequências mineradas, foram desenhados 59 pares de iniciadores para amplificá‑las. Os 59 iniciadores foram testados em 12 cafeeiros resistentes e 12 susceptíveis a H. vastatrix. Vinte e sete iniciadores resultaram em bandas únicas e bem definidas, enquanto um deles amplificou fragmento de DNA em todos os cafeeiros resistentes, mas não nos suscetíveis. Esse marcador molecular polimórfico amplificou uma região do DNA que corresponde a uma janela aberta de leitura parcial do genoma de C. arabica que codifica uma proteína de resistência a doenças. O marcador CARF 005 é capaz de diferenciar os cafeeiros analisados em resistentes e susceptíveis a H. vastatrix. |
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Palavras-Chave: |
Bioinformática; EST-PCR; Genes de resistência; Mineração de ados. |
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Thesagro: |
Coffea Arábica; Hemileia Vastatrix. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/44576/1/Marcadores-moleculares-derivados.pdf
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Marc: |
LEADER 01903naa a2200265 a 4500
001 1904175
005 2011-10-26
008 2011 bl uuuu u00u1 u #d
100 1 $aALVARENGA, S. M.
245 $aMarcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugem.$h[electronic resource]
260 $c2011
520 $aO objetivo deste trabalho foi identificar marcadores moleculares relacionados à resistência do cafeeiro (Coffea arabica) à ferrugem (Hemileia vastatrix). Foram identificadas sequências de DNA potencialmente envolvidas na resistência do cafeeiro a doenças, por meio de análise ?in silico?, a partir das informações geradas pelo Projeto Brasileiro do Genoma Café. A partir das sequências mineradas, foram desenhados 59 pares de iniciadores para amplificá‑las. Os 59 iniciadores foram testados em 12 cafeeiros resistentes e 12 susceptíveis a H. vastatrix. Vinte e sete iniciadores resultaram em bandas únicas e bem definidas, enquanto um deles amplificou fragmento de DNA em todos os cafeeiros resistentes, mas não nos suscetíveis. Esse marcador molecular polimórfico amplificou uma região do DNA que corresponde a uma janela aberta de leitura parcial do genoma de C. arabica que codifica uma proteína de resistência a doenças. O marcador CARF 005 é capaz de diferenciar os cafeeiros analisados em resistentes e susceptíveis a H. vastatrix.
650 $aCoffea Arábica
650 $aHemileia Vastatrix
653 $aBioinformática
653 $aEST-PCR
653 $aGenes de resistência
653 $aMineração de ados
700 1 $aCAIXETA, E. T.
700 1 $aHUFNAGEL, B.
700 1 $aTHIEBAUT, F.
700 1 $aZAMBOLIM, E. M.
700 1 $aZAMBOLIM, L.
700 1 $aSAKIYAMA, N. S.
773 $tPesquisa Agropecuária Brasileira. Brasília$gv. 46, n.8, p. 890-898, ago. 2011.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/11/2022 |
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Data da última atualização: |
30/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NOVATO, T. P.; MILHOMEM, M. N.; MARCHESINI, P. B. C.; COUTINHO, A. L.; SILVA, I. S.; PERINOTTO, W. M. de S.; PRATA, M. C. de A.; FERREIRA, L. L.; LOPES, W. D. Z.; COSTA-JÚNIOR, L. M.; MONTEIRO, C. M. de O. |
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Afiliação: |
TATIANE PINHEIRO NOVATO, Universidade Federal Rural do Rio de Janeiro; MARLISE NEVES MILHOMEM, Universidade Federal do Maranhão; PAULA BARROSO CRUZ MARCHESINI, Universidade Federal de Goiás; ANA LÚCIA COUTINHO, Universidade Federal de Goiás; ISABELA SANTOS SILVA, Universidade Federal de Goiás; WENDELL MARCELO DE SOUZA PERINOTTO, Universidade Federal do Recôncavo da Bahia; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; LORENA LOPES FERREIRA, Universidade Federal de Minas Gerais; WELBER DANIEL ZANETTI LOPES, Universidade Federal de Goiás; LÍVIO MARTINS COSTA-JÚNIOR, Universidade Federal do Maranhão; CAIO MARCIO DE OLIVEIRA MONTEIRO, Universidade Federal de Goiás. |
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Título: |
Acaricidal activity of carvacrol and thymol on acaricide-resistant Rhipicephalus microplus (Acari: Ixodidae) populations and combination with cypermethrin: is there cross-resistance and synergism? |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 310, 109787, 2022. |
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DOI: |
https://doi.org/10.1016/j.vetpar.2022.109787 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the acaricidal activity of thymol and carvacrol on Rhipicephalus microplus populations with different resistance profiles and investigated the synergistic effect of combinations of these monoterpenes with cypermethrin. The adult immersion test (AIT) was used to characterize the susceptibility of tick populations (45 field populations) to synthetic acaricides: deltamethrin, amitraz and chlorfenvinphos. The larval packet test (LPT) was used to determine the LC50 values for thymol (25 tick populations) and carvacrol (20 tick populations). The susceptible strain Porto Alegre (POA) was used as a reference for calculating the resistance ratio (RR). Subsequently, larval immersion tests (LIT) were performed with combinations of cypermethrin with thymol or carvacrol to assess a synergistic effect. In the AIT, deltamethrin showed efficacy > 90% in one (2.2%) population tested (mean: 12.1 and 11.1 for populations 1?25 and 26?40, respectively), whereas amitraz and chlorfenvinphos showed efficacy > 90% for two (4.4%) populations (mean: 61.3 and 47.3 for populations 1-25 and 26-40, respectively) and eight (17.7%) populations (mean: 69.7 and 59.7 for populations 1?25 and 26?40, respectively). In the LPT, the LC50 values for thymol and carvacrol varied from 0.67 to 2.12 mg/mL and 0.55?3.21 mg/mL, with an average LC50 for populations of 1.49 and 1.75 mg/mL, respectively. For thymol, no resistance was observed in any of the populations, values of RR50 > 1.5. There was no correlation between the LC50 values for thymol and the efficacy of the chemical acaricides tested. Regarding carvacrol, for only one tick population had the value of RR50 > 1.5, indicating an incipient resistance. No correlation was observed between the LC50 values for carvacrol and the efficacy of tested acaricides. The combination of thymol and carvacrol with cypermethrin showed a synergistic effect in the resistant population (Jaguar- thymol 4.19 and carvacrol 3.67), and no synergistic interaction were showed in the susceptible population. Answering the questions we conclude that: 1 - The comparison between the LC50 values for thymol and carvacrol in field populations and the susceptible strain POA suggests the absence of cross-resistance (ticks and terpenes), and the differences between the LC50 values for thymol and carvacrol in the different R. microplus populations are inherent to the characteristics of each population tested; 2 - the combination of thymol or carvacrol with cypermethrin showed a synergistic effect with different activity according to the population of ticks. MenosThis study evaluated the acaricidal activity of thymol and carvacrol on Rhipicephalus microplus populations with different resistance profiles and investigated the synergistic effect of combinations of these monoterpenes with cypermethrin. The adult immersion test (AIT) was used to characterize the susceptibility of tick populations (45 field populations) to synthetic acaricides: deltamethrin, amitraz and chlorfenvinphos. The larval packet test (LPT) was used to determine the LC50 values for thymol (25 tick populations) and carvacrol (20 tick populations). The susceptible strain Porto Alegre (POA) was used as a reference for calculating the resistance ratio (RR). Subsequently, larval immersion tests (LIT) were performed with combinations of cypermethrin with thymol or carvacrol to assess a synergistic effect. In the AIT, deltamethrin showed efficacy > 90% in one (2.2%) population tested (mean: 12.1 and 11.1 for populations 1?25 and 26?40, respectively), whereas amitraz and chlorfenvinphos showed efficacy > 90% for two (4.4%) populations (mean: 61.3 and 47.3 for populations 1-25 and 26-40, respectively) and eight (17.7%) populations (mean: 69.7 and 59.7 for populations 1?25 and 26?40, respectively). In the LPT, the LC50 values for thymol and carvacrol varied from 0.67 to 2.12 mg/mL and 0.55?3.21 mg/mL, with an average LC50 for populations of 1.49 and 1.75 mg/mL, respectively. For thymol, no resistance was observed in any of the populations, values of RR50 > 1.5. There was no ... Mostrar Tudo |
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Palavras-Chave: |
Cipermetrina; Clorfenvinfos. |
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Thesagro: |
Acaricida; Bovino; Carrapato. |
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Thesaurus NAL: |
Amitraz. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03661naa a2200325 a 4500
001 2148100
005 2022-11-30
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetpar.2022.109787$2DOI
100 1 $aNOVATO, T. P.
245 $aAcaricidal activity of carvacrol and thymol on acaricide-resistant Rhipicephalus microplus (Acari$bIxodidae) populations and combination with cypermethrin: is there cross-resistance and synergism?$h[electronic resource]
260 $c2022
520 $aThis study evaluated the acaricidal activity of thymol and carvacrol on Rhipicephalus microplus populations with different resistance profiles and investigated the synergistic effect of combinations of these monoterpenes with cypermethrin. The adult immersion test (AIT) was used to characterize the susceptibility of tick populations (45 field populations) to synthetic acaricides: deltamethrin, amitraz and chlorfenvinphos. The larval packet test (LPT) was used to determine the LC50 values for thymol (25 tick populations) and carvacrol (20 tick populations). The susceptible strain Porto Alegre (POA) was used as a reference for calculating the resistance ratio (RR). Subsequently, larval immersion tests (LIT) were performed with combinations of cypermethrin with thymol or carvacrol to assess a synergistic effect. In the AIT, deltamethrin showed efficacy > 90% in one (2.2%) population tested (mean: 12.1 and 11.1 for populations 1?25 and 26?40, respectively), whereas amitraz and chlorfenvinphos showed efficacy > 90% for two (4.4%) populations (mean: 61.3 and 47.3 for populations 1-25 and 26-40, respectively) and eight (17.7%) populations (mean: 69.7 and 59.7 for populations 1?25 and 26?40, respectively). In the LPT, the LC50 values for thymol and carvacrol varied from 0.67 to 2.12 mg/mL and 0.55?3.21 mg/mL, with an average LC50 for populations of 1.49 and 1.75 mg/mL, respectively. For thymol, no resistance was observed in any of the populations, values of RR50 > 1.5. There was no correlation between the LC50 values for thymol and the efficacy of the chemical acaricides tested. Regarding carvacrol, for only one tick population had the value of RR50 > 1.5, indicating an incipient resistance. No correlation was observed between the LC50 values for carvacrol and the efficacy of tested acaricides. The combination of thymol and carvacrol with cypermethrin showed a synergistic effect in the resistant population (Jaguar- thymol 4.19 and carvacrol 3.67), and no synergistic interaction were showed in the susceptible population. Answering the questions we conclude that: 1 - The comparison between the LC50 values for thymol and carvacrol in field populations and the susceptible strain POA suggests the absence of cross-resistance (ticks and terpenes), and the differences between the LC50 values for thymol and carvacrol in the different R. microplus populations are inherent to the characteristics of each population tested; 2 - the combination of thymol or carvacrol with cypermethrin showed a synergistic effect with different activity according to the population of ticks.
650 $aAmitraz
650 $aAcaricida
650 $aBovino
650 $aCarrapato
653 $aCipermetrina
653 $aClorfenvinfos
700 1 $aMILHOMEM, M. N.
700 1 $aMARCHESINI, P. B. C.
700 1 $aCOUTINHO, A. L.
700 1 $aSILVA, I. S.
700 1 $aPERINOTTO, W. M. de S.
700 1 $aPRATA, M. C. de A.
700 1 $aFERREIRA, L. L.
700 1 $aLOPES, W. D. Z.
700 1 $aCOSTA-JÚNIOR, L. M.
700 1 $aMONTEIRO, C. M. de O.
773 $tVeterinary Parasitology$gv. 310, 109787, 2022.
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