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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
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Data corrente: |
03/01/1996 |
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Data da última atualização: |
03/01/1996 |
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Autoria: |
LIU, Z.-W.; JARRET, R. L.; DUNCAN, R. R.; SRESOVICH, S. |
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Afiliação: |
University of Georgia. |
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Título: |
Genetic relationships and variation among ecotypes of seashore paspalum (Paspalum vaginatum) determined by random amplified polymorphic DNA markers. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Genome, v.37, p.1011-1017, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Random amplified polymorphic DNA (RAPD) markers were used to assess genetic relationships and variation among ecotypes of the turfgrass seashore paspalum (Paspalum vaginatum Swartz). Vegetative tissues or seeds of 46 seashore paspalum ecotypes were obtained from various locations in the United States, Argentina, and South Africa. Leaf DNA extracts were screened for RAPD markers using 34 10-mer random primers. A total of 195 reproducible RAPD fragments were observed, with an average of six fragments per primer. One hundred and sixty-nine fragments (87% of the total observed) were polymorphic, among which 27 fragments (16%) were present in three or less ecotypes, indicating the occurrence of a high level of genetic variation among the examined accessions of this species. Cluster analysis (UPGMA) and principal coordinates analysis were performed on the RAPD data set. The results illustrate genetic relationships among the 46 ecotypes, and between ecotypes and their geographical origins. Ecotypes from southern Africa could be differentiated from the U.S. and most of the Argentinean ecotypes. With a few exceptions, ecotypes collected from Argentina, Hawaii, Florida, and Texas were separated into distinct clusters. |
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Palavras-Chave: |
Genetic diversity; Phenetic analysis; RAPDs. |
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Thesagro: |
DNA; Genética. |
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Thesaurus Nal: |
Paspalum; Paspalum vaginatum; polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01956naa a2200253 a 4500
001 1075578
005 1996-01-03
008 1994 bl --- 0-- u #d
100 1 $aLIU, Z.-W.
245 $aGenetic relationships and variation among ecotypes of seashore paspalum (Paspalum vaginatum) determined by random amplified polymorphic DNA markers.
260 $c1994
520 $aRandom amplified polymorphic DNA (RAPD) markers were used to assess genetic relationships and variation among ecotypes of the turfgrass seashore paspalum (Paspalum vaginatum Swartz). Vegetative tissues or seeds of 46 seashore paspalum ecotypes were obtained from various locations in the United States, Argentina, and South Africa. Leaf DNA extracts were screened for RAPD markers using 34 10-mer random primers. A total of 195 reproducible RAPD fragments were observed, with an average of six fragments per primer. One hundred and sixty-nine fragments (87% of the total observed) were polymorphic, among which 27 fragments (16%) were present in three or less ecotypes, indicating the occurrence of a high level of genetic variation among the examined accessions of this species. Cluster analysis (UPGMA) and principal coordinates analysis were performed on the RAPD data set. The results illustrate genetic relationships among the 46 ecotypes, and between ecotypes and their geographical origins. Ecotypes from southern Africa could be differentiated from the U.S. and most of the Argentinean ecotypes. With a few exceptions, ecotypes collected from Argentina, Hawaii, Florida, and Texas were separated into distinct clusters.
650 $aPaspalum
650 $aPaspalum vaginatum
650 $apolymerase chain reaction
650 $aDNA
650 $aGenética
653 $aGenetic diversity
653 $aPhenetic analysis
653 $aRAPDs
700 1 $aJARRET, R. L.
700 1 $aDUNCAN, R. R.
700 1 $aSRESOVICH, S.
773 $tGenome$gv.37, p.1011-1017, 1994.
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Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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